FRS2 (NM_001278354) Human Tagged ORF Clone

CAT#: RC238621

FRS2 (myc-DDK-tagged) - Human fibroblast growth factor receptor substrate 2 (FRS2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FRS2
• Synonyms: FRS1A; FRS2A; FRS2alpha; SNT; SNT-1; SNT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238621 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTAGCTGTTGTAGCTGTCCAGATAAAGACACTGTCCCAGATAACCATCGGAACAAGTTTAAGGTCA
  TTAATGTGGATGATGATGGGAATGAGTTAGGTTCTGGCATAATGGAACTTACAGACACAGAACTGATTTT
  ATACACCCGCAAACGTGACTCAGTAAAATGGCACTACCTCTGCCTGCGACGCTATGGCTATGACTCGAAT
  CTCTTTTCTTTTGAAAGTGGTCGAAGGTGTCAAACTGGACAAGGAATCTTTGCCTTTAAGTGTGCCCGTG
  CAGAAGAATTATTTAACATGTTGCAAGAGATTATGCAAAATAATAGTATAAATGTGGTGGAAGAGCCAGT
  TGTAGAAAGAAATAATCATCAGACAGAATTGGAAGTCCCTAGAACACCTCGAACACCTACAACTCCAGGA
  TTTGCTGCTCAGAACTTACCTAATGGATATCCCCGATATCCCTCATTTGGAGATGCTTCATCCCATCCGT
  CAAGCAGACATCCTTCTGTGGGAAGTGCTCGCCTGCCTTCAGTAGGGGAAGAATCTACACATCCTTTGCT
  TGTGGCTGAGGAACAAGTACATACCTATGTCAACACTACAGGTGTGCAAGAAGAGCGGAAAAACCGCACA
  AGTGTGCATGTTCCATTGGAGGCAAGGGTTTCTAACGCTGAAAGCAGCACACCAAAAGAAGAACCAAGTA
  GTATTGAGGACAGGGATCCTCAGATTCTTCTTGAACCTGAAGGAGTCAAATTTGTTTTAGGGCCAACCCC
  TGTTCAAAAGCAGTTAATGGAAAAAGAGAAACTGGAGCAACTTGGAAGAGATCAAGTTAGTGGAAGTGGA
  GCAAATAACACAGAATGGGACACTGGCTATGACAGTGATGAACGAAGAGATGCACCCTCTGTTAACAAAC
  TGGTGTATGAAAATATAAATGGGCTATCTATCCCTAGTGCCTCAGGGGTCAGGAGAGGTCGTCTGACATC
  CACCAGTACCTCAGATACCCAGAATATCAACAACTCAGCTCAGAGAAGAACTGCATTATTAAACTATGAA
  AATCTACCATCTTTGCCTCCTGTTTGGGAAGCCCGCAAGCTAAGTAGGGATGAAGATGACAATTTAGGAC
  CAAAGACCCCATCTCTAAATGGCTACCATAATAATCTAGATCCAATGCATAACTATGTAAATACAGAGAA
  TGTAACAGTGCCAGCAAGTGCTCACAAAATAGAATATTCAAGGCGTCGGGACTGTACACCAACAGTCTTT
  AACTTTGATATCAGACGCCCAAGTTTAGAACACAGGCAGCTTAATTACATACAGGTTGACTTGGAAGGTG
  GCAGTGACTCTGACAACCCTCAGACTCCAAAAACGCCTACAACTCCCCTTCCACAAACCCCTACCAGGCG
  CACAGAGCTGTATGCCGTGATAGACATCGAGAGAACTGCTGCTATGTCAAATTTGCAGAAAGCACTGCCA
  CGAGATGATGGTACATCTAGGAAAACTAGACACAATAGTACTGATCTGCCCATGTTAGCCTGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238621 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTRKRDSVKWHYLCLRRYGYDSN
  LFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVERNNHQTELEVPRTPRTPTTPG
  FAAQNLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTTGVQEERKNRT
  SVHVPLEARVSNAESSTPKEEPSSIEDRDPQILLEPEGVKFVLGPTPVQKQLMEKEKLEQLGRDQVSGSG
  ANNTEWDTGYDSDERRDAPSVNKLVYENINGLSIPSASGVRRGRLTSTSTSDTQNINNSAQRRTALLNYE
  NLPSLPPVWEARKLSRDEDDNLGPKTPSLNGYHNNLDPMHNYVNTENVTVPASAHKIEYSRRRDCTPTVF
  NFDIRRPSLEHRQLNYIQVDLEGGSDSDNPQTPKTPTTPLPQTPTRRTELYAVIDIERTAAMSNLQKALP
  RDDGTSRKTRHNSTDLPMLAWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278354
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278354.1, NP_001265283.1
• RefSeq Size: 6929 bp
• RefSeq ORF: 1527 bp
• Locus ID: 10818
• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway
• MW: 57.5 kDa