EYA3 (NM_001282562) Human Tagged ORF Clone

CAT#: RC238699

EYA3 (myc-DDK-tagged) - Human EYA transcriptional coactivator and phosphatase 3 (EYA3), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EYA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238699 representing NM_001282562
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATGCACCGATTACATCCCTCGCTCATCCAATGATTATACCTCACAAATGTATTCTGCAAAACCTT
  ATGCACATATTCTCTCAGTTCCTGTTTCGGAAACTGCTTACCCTGGACAGACTCAATACCAGACACTACA
  GCAGACTCAACCCTATGCTGTCTACCCTCAGGCAACCCAAACGTATGGACTACCTCCTTTTGGTGCATTG
  TGGCCAGGTATGAAACCTGAAAGTGGTTTAATTCAGACTCCATCTCCAAGTCAACACAGTGTTCTTACCT
  GCACTACAGGGTTAACCACAAGCCAGCCAAGCCCAGCACATTATTCTTATCCCATTCAAGCTTCAAGCAC
  AAATGCCAGCCTGATATCTACTTCTTCTACAATTGCCAATATTCCAGCAGCAGCAGTAGCCAGCATCTCA
  AACCAGGATTATCCCACCTATACTATTCTTGGTCAGAATCAGTACCAGGCCTGCTACCCCAGCTCCAGCT
  TTGGAGTCACAGGTCAGACTAACAGTGATGCAGAGAGCACCACATTAGCAGCAACCACATACCAGTCGGA
  GAAGCCTAGTGTCATGGCGCCTGCACCTGCAGCACAGAGACTTTCCTCTGGAGACCCTTCTACAAGTCCA
  TCTTTGTCCCAGACTACACCAAGTAAAGATACTGATGATCAGTCCAGGAAAAACATGACTAGCAAGAACC
  GGGGCAAGAGGAAAGCTGATGCCACTTCTTCCCAAGACAGTGAATTAGAACGGGTATTTCTGTGGGACTT
  GGATGAAACCATCATCATCTTCCACTCACTTCTTACTGGATCCTATGCCCAGAAATATGGAAAGGACCCA
  ACAGTAGTGATTGGCTCAGGTTTAACAATGGAAGAAATGATTTTTGAAGTGGCTGATACTCATCTATTTT
  TCAATGACTTAGAGGAGTGTGACCAGGTACATGTGGAAGATGTGGCTTCTGATGACAATGGCCAAGACTT
  GAGCAACTACAGTTTCTCAACAGATGGTTTCAGTGGCTCAGGAGGTAGTGGCAGCCATGGTTCATCTGTG
  GGTGTTCAGGGAGGTGTGGACTGGATGAGGAAACTAGCTTTCCGCTACCGGAAAGTGAGAGAAATCTATG
  ATAAGCATAAAAGCAACGTGGGTGGTCTCCTCAGTCCCCAGAGGAAGGAAGCACTGCAGAGATTAAGAGC
  AGAAATTGAAGTTTTAACAGATTCCTGGTTAGGAACTGCATTAAAGTCCTTACTTCTCATCCAGTCCAGA
  AAGAATTGTGTGAATGTTCTGATCACTACCACCCAGCTGGTTCCAGCCCTGGCCAAGGTTCTCCTATATG
  GACTAGGAGAAATATTTCCTATTGAGAACATCTATAGTGCTACCAAAATTGGTAAGGAGAGCTGCTTTGA
  GAGAATTGTGTCAAGGTTTGGAAAGAAAGTCACATATGTAGTGATTGGAGATGGACGAGATGAAGAAATT
  GCAGCCAAACAGCACAACATGCCTTTCTGGAGGATCACAAACCATGGAGACCTAGTATCCCTTCACCAGG
  CTTTAGAGCTTGATTTTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238699 representing NM_001282562
  Red=Cloning site Green=Tags(s)
  
  MTCTDYIPRSSNDYTSQMYSAKPYAHILSVPVSETAYPGQTQYQTLQQTQPYAVYPQATQTYGLPPFGAL
  WPGMKPESGLIQTPSPSQHSVLTCTTGLTTSQPSPAHYSYPIQASSTNASLISTSSTIANIPAAAVASIS
  NQDYPTYTILGQNQYQACYPSSSFGVTGQTNSDAESTTLAATTYQSEKPSVMAPAPAAQRLSSGDPSTSP
  SLSQTTPSKDTDDQSRKNMTSKNRGKRKADATSSQDSELERVFLWDLDETIIIFHSLLTGSYAQKYGKDP
  TVVIGSGLTMEEMIFEVADTHLFFNDLEECDQVHVEDVASDDNGQDLSNYSFSTDGFSGSGGSGSHGSSV
  GVQGGVDWMRKLAFRYRKVREIYDKHKSNVGGLLSPQRKEALQRLRAEIEVLTDSWLGTALKSLLLIQSR
  KNCVNVLITTTQLVPALAKVLLYGLGEIFPIENIYSATKIGKESCFERIVSRFGKKVTYVVIGDGRDEEI
  AAKQHNMPFWRITNHGDLVSLHQALELDFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282562
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282562.1, NP_001269491.1
• RefSeq Size: 5961 bp
• RefSeq ORF: 1563 bp
• Locus ID: 2140
• Cytogenetics: 1p35.3
• Protein Families: Phosphatase, Transcription Factors
• MW: 57.3 kDa
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]'