EYA3 (NM_001282561) Human Tagged ORF Clone

CAT#: RC238730

EYA3 (myc-DDK-tagged) - Human EYA transcriptional coactivator and phosphatase 3 (EYA3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EYA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238730 representing NM_001282561
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAGAGCAAGATTTACCAGAGCAACCAGTGAAAAAAGCCAAGATGCAGGAATCAGGAGAGCAAA
  CTATAAGTCAAGTAAGCAATCCAGATGTCAGTGATCAGAAGCCTGAAACATCAAGCCTTGCTTCAAACCT
  TCCCATGTCAGAGGAAATTATGACATGCACCGATTACATCCCTCGCTCATCCAATGATTATACCTCACAA
  ATGTATTCTGCAAAACCTTATGCACATATTCTCTCAGTTCCTGTTTCGGAAACTGCTTACCCTGGACAGA
  CTCAATACCAGACACTACAGCAGACTCAACCCTATGCTGTCTACCCTCAGGCAACCCAAACGTATGGACT
  ACCTCCTTTTGCTTCAAGCACAAATGCCAGCCTGATATCTACTTCTTCTACAATTGCCAATATTCCAGCA
  GCAGCAGTAGCCAGCATCTCAAACCAGGATTATCCCACCTATACTATTCTTGGTCAGAATCAGTACCAGG
  CCTGCTACCCCAGCTCCAGCTTTGGAGTCACAGGTCAGACTAACAGTGATGCAGAGAGCACCACATTAGC
  AGCAACCACATACCAGTCGGAGAAGCCTAGTGTCATGGCGCCTGCACCTGCAGCACAGAGACTTTCCTCT
  GGAGACCCTTCTACAAGTCCATCTTTGTCCCAGACTACACCAAGTAAAGATACTGATGATCAGTCCAGGA
  AAAACATGACTAGCAAGAACCGGGGCAAGAGGAAAGCTGATGCCACTTCTTCCCAAGACAGTGAATTAGA
  ACGGGTATTTCTGTGGGACTTGGATGAAACCATCATCATCTTCCACTCACTTCTTACTGGATCCTATGCC
  CAGAAATATGGAAAGGACCCAACAGTAGTGATTGGCTCAGGTTTAACAATGGAAGAAATGATTTTTGAAG
  TGGCTGATACTCATCTATTTTTCAATGACTTAGAGGAGTGTGACCAGGTACATGTGGAAGATGTGGCTTC
  TGATGACAATGGCCAAGACTTGAGCAACTACAGTTTCTCAACAGATGGTTTCAGTGGCTCAGGAGGTAGT
  GGCAGCCATGGTTCATCTGTGGGTGTTCAGGGAGGTGTGGACTGGATGAGGAAACTAGCTTTCCGCTACC
  GGAAAGTGAGAGAAATCTATGATAAGCATAAAAGCAACGTGGGTGGTCTCCTCAGTCCCCAGAGGAAGGA
  AGCACTGCAGAGATTAAGAGCAGAAATTGAAGTTTTAACAGATTCCTGGTTAGGAACTGCATTAAAGTCC
  TTACTTCTCATCCAGTCCAGAAAGAATTGTGTGAATGTTCTGATCACTACCACCCAGCTGGTTCCAGCCC
  TGGCCAAGGTTCTCCTATATGGACTAGGAGAAATATTTCCTATTGAGAACATCTATAGTGCTACCAAAAT
  TGGTAAGGAGAGCTGCTTTGAGAGAATTGTGTCAAGGTTTGGAAAGAAAGTCACATATGTAGTGATTGGA
  GATGGACGAGATGAAGAAATTGCAGCCAAACAGCACAACATGCCTTTCTGGAGGATCACAAACCATGGAG
  ACCTAGTATCCCTTCACCAGGCTTTAGAGCTTGATTTTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238730 representing NM_001282561
  Red=Cloning site Green=Tags(s)
  
  MEEEQDLPEQPVKKAKMQESGEQTISQVSNPDVSDQKPETSSLASNLPMSEEIMTCTDYIPRSSNDYTSQ
  MYSAKPYAHILSVPVSETAYPGQTQYQTLQQTQPYAVYPQATQTYGLPPFASSTNASLISTSSTIANIPA
  AAVASISNQDYPTYTILGQNQYQACYPSSSFGVTGQTNSDAESTTLAATTYQSEKPSVMAPAPAAQRLSS
  GDPSTSPSLSQTTPSKDTDDQSRKNMTSKNRGKRKADATSSQDSELERVFLWDLDETIIIFHSLLTGSYA
  QKYGKDPTVVIGSGLTMEEMIFEVADTHLFFNDLEECDQVHVEDVASDDNGQDLSNYSFSTDGFSGSGGS
  GSHGSSVGVQGGVDWMRKLAFRYRKVREIYDKHKSNVGGLLSPQRKEALQRLRAEIEVLTDSWLGTALKS
  LLLIQSRKNCVNVLITTTQLVPALAKVLLYGLGEIFPIENIYSATKIGKESCFERIVSRFGKKVTYVVIG
  DGRDEEIAAKQHNMPFWRITNHGDLVSLHQALELDFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282561
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282561.1, NP_001269490.1
• RefSeq Size: 5903 bp
• RefSeq ORF: 1584 bp
• Locus ID: 2140
• Cytogenetics: 1p35.3
• Protein Families: Phosphatase, Transcription Factors
• MW: 58.3 kDa
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]'