Complement factor 8 beta (C8B) (NM_001278543) Human Tagged ORF Clone

CAT#: RC238796

C8B (myc-DDK-tagged) - Human complement component 8, beta polypeptide (C8B), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C8B
• Synonyms: C82
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238796 representing NM_001278543
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAGTGTGGATGTTACCCTGATGCCCATTGATTGTGAGCTGTCTAGTTGGTCCTCTTGGACCACAT
  GTGACCCCTGTCAGAAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAACC
  GTGCAACTTCTCTGACAAGGAAGTCGAAGACTGTGTTACCAACAGACCATGCAGAAGTCAAGTGCGATGT
  GAAGGCTTTGTGTGTGCACAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGCAATGGGGACAATGACT
  GTGGAGACCAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAAAAATGTCAGCATGAAATGGACCAATA
  CTGGGGAATTGGCAGTCTGGCCAGTGGGATAAATTTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGAT
  CACAGGTATTATGCAGGTGGATGCTCCCCGCATTACATCCTGAACACGAGGTTTAGGAAGCCCTACAATG
  TGGAAAGCTACACGCCACAGACCCAAGGCAAATACGAATTCATATTAAAAGAGTATGAATCATACTCAGA
  TTTTGAACGCAATGTCACAGAGAAAATGGCAAGCAAGTCTGGTTTCAGTTTTGGTTTTAAAATACCTGGA
  ATATTTGAACTTGGCATCAGTAGTCAAAGTGATCGAGGCAAACACTATATTAGGAGAACCAAACGATTCT
  CTCATACTAAAAGCGTATTTCTGCATGCACGCTCTGACCTTGAAGTAGCACATTACAAGCTGAAACCCAG
  AAGCCTCATGCTCCATTACGAGTTCCTTCAGAGAGTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGAA
  TACAGAGATCTCTTCCGTGATTTTGGGACCCACTACATCACAGAGGCTGTGCTTGGGGGCATTTATGAAT
  ACACCCTCGTTATGAACAAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACAACGTCCATGCCTGTGC
  CAAAAATGATTTTAAAATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGGTGTGTCTGTAGGCAAA
  TGCAGAGGTATTCTGAATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTGGAGGACTTGGTGGTCC
  TGGTACGAGGAGGGGCAAGTGAGCACATCACCACCCTGGCATACCAGGAGCTGCCGACGGCGGACCTGAT
  GCAGGAGTGGGGAGACGCTGTGCAGTACAACCCAGCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGAA
  CTAGTGACAGCCACAGATTTTGCCTATTCCAGCACAGTGAGGCAGAACATGAAGCAGGCACTGGAGGAGT
  TCCAGAAGGAAGTTAGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCCCTGTCCTGAAAGGATC
  ACGCTGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTCCTATCGGAAGAATACC
  CCCATTGATGGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGACGTAAGACAAGACAAA
  GGCAGTGTAACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGTTCAGGCCCTGCTTCAGAAACACTTGA
  CTGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238796 representing NM_001278543
  Red=Cloning site Green=Tags(s)
  
  MRSVDVTLMPIDCELSSWSSWTTCDPCQKKRYRYAYLLQPSQFHGEPCNFSDKEVEDCVTNRPCRSQVRC
  EGFVCAQTGRCVNRRLLCNGDNDCGDQSDEANCRRIYKKCQHEMDQYWGIGSLASGINLFTNSFEGPVLD
  HRYYAGGCSPHYILNTRFRKPYNVESYTPQTQGKYEFILKEYESYSDFERNVTEKMASKSGFSFGFKIPG
  IFELGISSQSDRGKHYIRRTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGE
  YRDLFRDFGTHYITEAVLGGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGK
  CRGILNEIKDRNKRDTMVEDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYE
  LVTATDFAYSSTVRQNMKQALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNT
  PIDGKWNCWSNWSSCSGRRKTRQRQCNNPPPQNGGSPCSGPASETLDCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278543
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278543.1, NP_001265472.1
• RefSeq Size: 2400 bp
• RefSeq ORF: 1620 bp
• Locus ID: 732
• Cytogenetics: 1p32.2
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• MW: 61.8 kDa
• Gene Summary: 'This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]'