GGA3 (NM_001291641) Human Tagged ORF Clone

CAT#: RC239205

GGA3 (myc-DDK-tagged) - Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239205 representing NM_001291641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAACTGTGGGAGGAGATTTCATAACGAAGTGGGGAAGTTCCGCTTTTTGAATGAGTTAATCAAAG
  TCGTCTCTCCAAAGTACCTGGGGGACAGGGTGTCTGAGAAAGTGAAGACCAAGGTTATTGAGCTGCTGTA
  CAGCTGGACCATGGCCCTGCCAGAAGAAGCAAAGATCAAAGACGCCTACCACATGCTGAAGAGACAGGGC
  ATAGTGCAGTCTGACCCACCAATTCCTGTGGATAGGACGCTGATCCCCTCTCCACCACCTCGTCCCAAAA
  ACCCTGTTTTTGATGATGAGGAGAAGTCCAAGCTTTTAGCCAAGCTGCTGAAAAGCAAAAACCCAGATGA
  CCTGCAGGAGGCCAACAAGCTCATCAAGTCCATGGTGAAGGAAGACGAGGCACGGATCCAGAAGGTGACC
  AAGCGTCTGCACACGTTAGAGGAAGTTAACAACAACGTGAGACTGCTCAGTGAGATGCTGCTTCATTACA
  GCCAGGAGGACTCTTCGGACGGGGACAGAGAGCTGATGAAGGAGCTGTTTGATCAGTGTGAGAACAAGAG
  GCGGACTTTATTTAAACTCGCCAGTGAGACTGAGGACAATGATAACAGTTTGGGGGACATCCTGCAAGCC
  AGTGACAACCTCTCCCGGGTCATCAACTCTTACAAAACAATTATTGAAGGGCAGGTCATCAATGGCGAGG
  TGGCTACCTTAACCCTGCCTGACTCGGAAGGAAACAGTCAGTGCAGTAACCAAGGCACGCTCATCGACCT
  TGCGGAGCTGGACACGACCAACAGTTTGTCCTCCGTGTTGGCCCCAGCACCTACTCCACCCTCCTCAGGC
  ATCCCAATCCTCCCTCCACCACCCCAGGCCTCAGGACCTCCACGGAGCCGCTCCTCTAGCCAGGCCGAGG
  CCACCCTGGGGCCCAGCAGCACAAGCAACGCCCTCTCCTGGCTGGACGAGGAGCTACTCTGCTTGGGCCT
  CGCCGACCCAGCCCCTAATGTTCCTCCCAAAGAGTCAGCTGGGAACAGCCAGTGGCACCTGCTCCAGAGG
  GAACAGTCCGACCTGGACTTCTTCAGCCCCAGGCCGGGGACCGCTGCCTGTGGCGCCTCCGATGCTCCTC
  TGCTCCAGCCCTCAGCCCCCTCCTCAAGCAGCTCCCAAGCTCCACTGCCGCCTCCCTTCCCAGCTCCTGT
  GGTCCCAGCCAGTGTTCCTGCCCCCAGTGCGGGCTCCTCCTTGTTTTCTACTGGAGTGGCCCCAGCCTTG
  GCCCCAAAAGTTGAGCCCGCAGTCCCTGGGCACCATGGCTTGGCGTTGGGCAACAGCGCGCTGCACCACC
  TGGATGCCCTCGATCAGCTTCTAGAAGAGGCCAAAGTGACCTCGGGCTTGGTGAAACCCACTACCTCCCC
  TCTCATCCCCACCACCACCCCAGCCAGGCCCCTCCTGCCCTTCTCCACGGGGCCCGGCAGCCCGCTCTTC
  CAGCCACTGAGTTTCCAGTCCCAGGGCAGCCCCCCGAAGGGGCCTGAGCTCTCCCTGGCCAGCATCCACG
  TGCCCCTGGAATCGATCAAGCCTAGCAGTGCCCTTCCTGTGACAGCCTACGATAAAAACGGCTTCCGCAT
  CCTCTTCCACTTTGCCAAGGAGTGTCCCCCAGGACGACCTGACGTGCTGGTGGTGGTGGTGTCCATGCTG
  AACACGGCTCCCTTACCTGTCAAGAGCATCGTGCTGCAGGCTGCAGTGCCCAAGTCAATGAAAGTGAAGT
  TGCAGCCACCTTCTGGGACAGAACTCTCTCCATTTAGCCCCATCCAGCCACCTGCAGCCATCACCCAGGT
  CATGTTGCTGGCCAATCCACTGAAGGAGAAGGTGCGGCTTCGGTATAAGCTGACCTTCGCCCTGGGGGAG
  CAGCTGAGCACAGAGGTGGGCGAGGTGGACCAGTTCCCTCCTGTGGAACAGTGGGGGAACCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239205 representing NM_001291641
  Red=Cloning site Green=Tags(s)
  
  MKNCGRRFHNEVGKFRFLNELIKVVSPKYLGDRVSEKVKTKVIELLYSWTMALPEEAKIKDAYHMLKRQG
  IVQSDPPIPVDRTLIPSPPPRPKNPVFDDEEKSKLLAKLLKSKNPDDLQEANKLIKSMVKEDEARIQKVT
  KRLHTLEEVNNNVRLLSEMLLHYSQEDSSDGDRELMKELFDQCENKRRTLFKLASETEDNDNSLGDILQA
  SDNLSRVINSYKTIIEGQVINGEVATLTLPDSEGNSQCSNQGTLIDLAELDTTNSLSSVLAPAPTPPSSG
  IPILPPPPQASGPPRSRSSSQAEATLGPSSTSNALSWLDEELLCLGLADPAPNVPPKESAGNSQWHLLQR
  EQSDLDFFSPRPGTAACGASDAPLLQPSAPSSSSSQAPLPPPFPAPVVPASVPAPSAGSSLFSTGVAPAL
  APKVEPAVPGHHGLALGNSALHHLDALDQLLEEAKVTSGLVKPTTSPLIPTTTPARPLLPFSTGPGSPLF
  QPLSFQSQGSPPKGPELSLASIHVPLESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVLVVVVSML
  NTAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLANPLKEKVRLRYKLTFALGE
  QLSTEVGEVDQFPPVEQWGNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291641
• ORF Size: 1953 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001291641.1, NP_001278570.1
• RefSeq Size: 4118
• RefSeq ORF: 1956
• Locus ID: 23163
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 70.6 kDa
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]