ZNF133 (NM_001283002) Human Tagged ORF Clone

CAT#: RC239231

ZNF133 (myc-DDK-tagged) - Human zinc finger protein 133 (ZNF133), transcript variant 10

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF133
• Synonyms: pHZ-13; pHZ-66; ZNF150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239231 representing NM_001283002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACACATGGCATTCAGGGATGTGGCTGTGGATTTCACCCAGGATGAGTGGAGGCTGCTGAGCCCTG
  CTCAAAGGACTCTGTACAGAGAGGTGATGCTGGAGAACTACAGCAACCTGGTCTCACTGGGAATTTCATT
  TTCTAAACCAGAACTCATCACCCAGCTGGAGCAAGGGAAAGAGACCTGGAGAGAGGAAAAAAAATGTTCA
  CCGGCAACCTGTCCAGCAGATCCAGAGCCAGAGCTCTACCTCGATCCTTTCTGCCCTCCGGGTTTCTCCA
  GTCAGAAATTCCCCATGCAGCATGTGCTGTGTAATCATCCCCCCTGGATCTTCACATGCTTGTGTGCAGA
  AGGTAACATCCAGCCTGGGGATCCGGGCCCAGGGGACCAGGAGAAGCAGCAACAAGCCTCTGAGGGGAGA
  CCCTGGAGTGATCAAGCAGAAGGTCCTGAGGGAGAAGGTGCCATGCCTTTGTTTGGAAGAACCAAGAAAA
  GGACTCTGGGAGCGTTCTCCAGGCCACCCCAGAGGCAGCCAGTCAGCTCTCGGAACGGCCTCAGAGGGGT
  GGAGTTAGAAGCCAGCCCAGCTCAGACAGGGAACCCTGAGGAAACAGACAAATTGTTGAAGAGGATAGAA
  GTCTTAGGATTTGGAACAGTCAACTGTGGAGAGTGTGGACTGAGCTTCAGCAAGATGACAAACCTGCTCA
  GTCACCAGCGGATACACTCAGGGGAGAAGCCCTACGTGTGTGGGGTATGTGAGAAGGGCTTCAGCCTAAA
  GAAGAGCCTCGCCAGACACCAGAAGGCACACTCGGGGGAGAAGCCAATTGTGTGCAGGGAGTGTGGACGA
  GGCTTTAACCGGAAGTCAACGCTAATCATACACGAACGGACACACTCCGGTGAGAAACCTTACATGTGCA
  GTGAGTGTGGGCGAGGCTTCAGCCAGAAGTCAAACCTCATCATACACCAGAGGACACACTCAGGGGAAAA
  GCCTTACGTGTGCCGGGAATGTGGCAAAGGCTTCAGCCAGAAGTCAGCTGTCGTGAGACACCAGAGGACA
  CACTTGGAGGAGAAGACCATCGTGTGCAGTGACTGTGGCCTGGGCTTCAGCGACAGGTCAAACCTCATCT
  CCCACCAGAGGACGCACTCTGGGGAGAAGCCCTACGCCTGCAAGGAGTGTGGGCGATGCTTCAGGCAGAG
  GACCACCCTTGTCAACCACCAGAGGACACACTCAAAGGAGAAGCCCTATGTGTGCGGGGTGTGTGGGCAC
  AGCTTCAGCCAGAATTCAACCCTCATCTCTCACAGGCGGACACACACTGGGGAGAAGCCGTATGTTTGTG
  GGGTGTGTGGGCGAGGCTTTAGTCTCAAGTCACACCTCAACAGACACCAGAACATACACTCAGGAGAGAA
  GCCCATTGTGTGCAAGGACTGTGGCCGGGGCTTCAGCCAGCAATCCAACCTCATCAGACACCAGAGGACG
  CACTCAGGCGAGAAGCCCATGGTGTGTGGGGAGTGCGGGCGAGGCTTCAGCCAGAAGTCAAACCTTGTTG
  CACACCAGAGGACGCACTCAGGGGAGAGGCCGTATGTGTGCCGAGAGTGCGGGCGAGGCTTTAGCCACCA
  GGCCGGTCTCATCAGGCACAAGCGGAAGCACTCGAGGGAGAAGCCCTACATGTGCAGGCAGTGTGGACTG
  GGCTTTGGCAATAAGTCAGCTCTAATTACACACAAGCGGGCTCACTCGGAAGAGAAGCCTTGTGTGTGCA
  GAGAGTGTGGCCAAGGCTTTCTCCAAAAGTCACACCTCACCTTACATCAAATGACACATACGGGGGAGAA
  GCCATATGTGTGCAAGACGTGTGGGCGGGGCTTCAGCCTCAAGTCTCACCTCAGCAGACACAGGAAGACC
  ACGTCTGTCCACCACAGACTGCCAGTGCAGCCCGACCCTGAGCCGTGTGCAGGGCAACCTTCGGATTCCT
  TATACTCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239231 representing NM_001283002
  Red=Cloning site Green=Tags(s)
  
  MAHMAFRDVAVDFTQDEWRLLSPAQRTLYREVMLENYSNLVSLGISFSKPELITQLEQGKETWREEKKCS
  PATCPADPEPELYLDPFCPPGFSSQKFPMQHVLCNHPPWIFTCLCAEGNIQPGDPGPGDQEKQQQASEGR
  PWSDQAEGPEGEGAMPLFGRTKKRTLGAFSRPPQRQPVSSRNGLRGVELEASPAQTGNPEETDKLLKRIE
  VLGFGTVNCGECGLSFSKMTNLLSHQRIHSGEKPYVCGVCEKGFSLKKSLARHQKAHSGEKPIVCRECGR
  GFNRKSTLIIHERTHSGEKPYMCSECGRGFSQKSNLIIHQRTHSGEKPYVCRECGKGFSQKSAVVRHQRT
  HLEEKTIVCSDCGLGFSDRSNLISHQRTHSGEKPYACKECGRCFRQRTTLVNHQRTHSKEKPYVCGVCGH
  SFSQNSTLISHRRTHTGEKPYVCGVCGRGFSLKSHLNRHQNIHSGEKPIVCKDCGRGFSQQSNLIRHQRT
  HSGEKPMVCGECGRGFSQKSNLVAHQRTHSGERPYVCRECGRGFSHQAGLIRHKRKHSREKPYMCRQCGL
  GFGNKSALITHKRAHSEEKPCVCRECGQGFLQKSHLTLHQMTHTGEKPYVCKTCGRGFSLKSHLSRHRKT
  TSVHHRLPVQPDPEPCAGQPSDSLYSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283002
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001283002.1, NP_001269931.1
• RefSeq Size: 2568 bp
• RefSeq ORF: 1974 bp
• Locus ID: 7692
• Cytogenetics: 20p11.23
• Protein Families: Transcription Factors
• MW: 74.2 kDa
• Gene Summary: ''