ZNF133 (NM_001283006) Human Tagged ORF Clone

CAT#: RC239246

ZNF133 (myc-DDK-tagged) - Human zinc finger protein 133 (ZNF133), transcript variant 14

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF133
• Synonyms: pHZ-13; pHZ-66; ZNF150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239246 representing NM_001283006
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCTGCCGGCCTTCAGGGCGCGCCTGGTGGCGCCGAGCATCCTGGGAGGTGTGGTCCGGGCGCTG
  TGCCGAGGATTCGGGGTAGTGTAGTCCTGGCGCCCCGCTGGAGGAGCTCCCCAGCTGGTGGCTGGAGCGA
  CCCCTTGTTCTTTGGTGGCGCTGAACTCATCACCCAGCTGGAGCAAGGGAAAGAGACCTGGAGAGAGGAA
  AAAAAATGTTCACCGGCAACCTGTCCAGCAGATCCAGAGCCAGAGCTCTACCTCGATCCTTTCTGCCCTC
  CGGGTTTCTCCAGTCAGAAATTCCCCATGCAGCATGTGCTGTGTAATCATCCCCCCTGGATCTTCACATG
  CTTGTGTGCAGAAGGTAACATCCAGCCTGGGGATCCGGGCCCAGGGGACCAGGAGAAGCAGCAACAAGCC
  TCTGAGGGGAGACCCTGGAGTGATCAAGCAGAAGGTCCTGAGGGAGAAGGTGCCATGCCTTTGTTTGGAA
  GAACCAAGAAAAGGACTCTGGGAGCGTTCTCCAGGCCACCCCAGAGGCAGCCAGTCAGCTCTCGGAACGG
  CCTCAGAGGGGTGGAGTTAGAAGCCAGCCCAGCTCAGACAGGGAACCCTGAGGAAACAGACAAATTGTTG
  AAGAGGATAGAAGTCTTAGGATTTGGAACAGTCAACTGTGGAGAGTGTGGACTGAGCTTCAGCAAGATGA
  CAAACCTGCTCAGTCACCAGCGGATACACTCAGGGGAGAAGCCCTACGTGTGTGGGGTATGTGAGAAGGG
  CTTCAGCCTAAAGAAGAGCCTCGCCAGACACCAGAAGGCACACTCGGGGGAGAAGCCAATTGTGTGCAGG
  GAGTGTGGACGAGGCTTTAACCGGAAGTCAACGCTAATCATACACGAACGGACACACTCCGGTGAGAAAC
  CTTACATGTGCAGTGAGTGTGGGCGAGGCTTCAGCCAGAAGTCAAACCTCATCATACACCAGAGGACACA
  CTCAGGGGAAAAGCCTTACGTGTGCCGGGAATGTGGCAAAGGCTTCAGCCAGAAGTCAGCTGTCGTGAGA
  CACCAGAGGACACACTTGGAGGAGAAGACCATCGTGTGCAGTGACTGTGGCCTGGGCTTCAGCGACAGGT
  CAAACCTCATCTCCCACCAGAGGACGCACTCTGGGGAGAAGCCCTACGCCTGCAAGGAGTGTGGGCGATG
  CTTCAGGCAGAGGACCACCCTTGTCAACCACCAGAGGACACACTCAAAGGAGAAGCCCTATGTGTGCGGG
  GTGTGTGGGCACAGCTTCAGCCAGAATTCAACCCTCATCTCTCACAGGCGGACACACACTGGGGAGAAGC
  CGTATGTTTGTGGGGTGTGTGGGCGAGGCTTTAGTCTCAAGTCACACCTCAACAGACACCAGAACATACA
  CTCAGGAGAGAAGCCCATTGTGTGCAAGGACTGTGGCCGGGGCTTCAGCCAGCAATCCAACCTCATCAGA
  CACCAGAGGACGCACTCAGGCGAGAAGCCCATGGTGTGTGGGGAGTGCGGGCGAGGCTTCAGCCAGAAGT
  CAAACCTTGTTGCACACCAGAGGACGCACTCAGGGGAGAGGCCGTATGTGTGCCGAGAGTGCGGGCGAGG
  CTTTAGCCACCAGGCCGGTCTCATCAGGCACAAGCGGAAGCACTCGAGGGAGAAGCCCTACATGTGCAGG
  CAGTGTGGACTGGGCTTTGGCAATAAGTCAGCTCTAATTACACACAAGCGGGCTCACTCGGAAGAGAAGC
  CTTGTGTGTGCAGAGAGTGTGGCCAAGGCTTTCTCCAAAAGTCACACCTCACCTTACATCAAATGACACA
  TACGGGGGAGAAGCCATATGTGTGCAAGACGTGTGGGCGGGGCTTCAGCCTCAAGTCTCACCTCAGCAGA
  CACAGGAAGACCACGTCTGTCCACCACAGACTGCCAGTGCAGCCCGACCCTGAGCCGTGTGCAGGGCAAC
  CTTCGGATTCCTTATACTCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239246 representing NM_001283006
  Red=Cloning site Green=Tags(s)
  
  MGAAGLQGAPGGAEHPGRCGPGAVPRIRGSVVLAPRWRSSPAGGWSDPLFFGGAELITQLEQGKETWREE
  KKCSPATCPADPEPELYLDPFCPPGFSSQKFPMQHVLCNHPPWIFTCLCAEGNIQPGDPGPGDQEKQQQA
  SEGRPWSDQAEGPEGEGAMPLFGRTKKRTLGAFSRPPQRQPVSSRNGLRGVELEASPAQTGNPEETDKLL
  KRIEVLGFGTVNCGECGLSFSKMTNLLSHQRIHSGEKPYVCGVCEKGFSLKKSLARHQKAHSGEKPIVCR
  ECGRGFNRKSTLIIHERTHSGEKPYMCSECGRGFSQKSNLIIHQRTHSGEKPYVCRECGKGFSQKSAVVR
  HQRTHLEEKTIVCSDCGLGFSDRSNLISHQRTHSGEKPYACKECGRCFRQRTTLVNHQRTHSKEKPYVCG
  VCGHSFSQNSTLISHRRTHTGEKPYVCGVCGRGFSLKSHLNRHQNIHSGEKPIVCKDCGRGFSQQSNLIR
  HQRTHSGEKPMVCGECGRGFSQKSNLVAHQRTHSGERPYVCRECGRGFSHQAGLIRHKRKHSREKPYMCR
  QCGLGFGNKSALITHKRAHSEEKPCVCRECGQGFLQKSHLTLHQMTHTGEKPYVCKTCGRGFSLKSHLSR
  HRKTTSVHHRLPVQPDPEPCAGQPSDSLYSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283006
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001283006.1, NP_001269935.1
• RefSeq Size: 2342 bp
• RefSeq ORF: 1986 bp
• Locus ID: 7692
• Cytogenetics: 20p11.23
• Protein Families: Transcription Factors
• MW: 73.7 kDa
• Gene Summary: ''