XPB (ERCC3) (NM_001303416) Human Tagged ORF Clone

CAT#: RC239397

ERCC3 (myc-DDK-tagged) - Human excision repair cross-complementation group 3 (ERCC3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ERCC3
• Synonyms: BTF2; GTF2H; RAD25; TFIIH; TTD2; XPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239397 representing NM_001303416
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGAAGGACGACCACACCTCCAGGCCCCTCTGGGTGGCTCCCGATGGCCATATCTTCTTGGAAG
  CCTTCTCTCCAGTTTACAAATATGCCCAAGACTTCTTGGTGGCTATTGCAGAGCCAGTGTGCCGACCAAC
  CCATGTGCATGAGTACAAACTAACTGCCTACTCCTTGTATGCAGCTGTCAGCGTTGGGCTGCAAACCAGT
  GACATCACCGAGTACCTCAGGAAGCTCAGCAAGACTGGAGTCCCTGATGGAATTATGCAGTTTATTAAGT
  TGTGTACTGTCAGCTATGGAAAAGTCAAGCTGGTCTTGAAGCACAACAGATACTTCGTTGAAAGTTGCCA
  CCCTGATGTAATCCAGCATCTTCTCCAGGACCCCGTGATCCGAGAATGCCGCTTAAGAAACTCTGAAGGG
  GAGGCCACTGAGCTCATCACAGAGACTTTCACAAGCAAATCTGCCATTTCTAAGACTGCTGAAAGCAGTG
  GTGGGCCCTCCACTTCCCGAGTGACAGATCCACAGGGTAAATCTGACATCCCCATGGACCTGTTTGACTT
  CTATGAGCAAATGGACAAGGATGAAGAAGAAGAAGAAGAGACACAGACAGTGTCTTTTGAAGTCAAGCAG
  GAAATGATTGAGGAACTCCAGAAACGTTGCATCCACCTGGAGTACCCTCTGTTGGCAGAATATGACTTCC
  GGAATGATTCTGTCAACCCTGATATCAACATTGACCTAAAGCCCACAGCTGTCCTCAGACCCTATCAGGA
  GAAGAGCTTGCGAAAGATGTTTGGAAACGGGCGTGCACGTTCGGGGGTCATTGTTCTTCCCTGCGGTGCT
  GGAAAGTCCCTGGTTGGTGTGACTGCTGCATGCACTGTCAGAAAACGCTGTCTGGTGCTGGGCAACTCAG
  CTGTTTCTGTGGAGCAGTGGAAAGCCCAGTTCAAGATGTGGTCCACCATTGACGACAGCCAGATCTGCCG
  GTTCACCTCCGATGCCAAGGACAAGCCCATCGGCTGCTCCGTTGCCATTAGCACCTACTCCATGCTGGGC
  CACACCACCAAAAGGTCCTGGGAGGCCGAGCGAGTCATGGAGTGGCTCAAGACCCAGGAGTGGGGCCTCA
  TGATCCTGGATGAAGTGCACACCATACCAGCCAAGATGTTCCGAAGGGTGCTCACCATCGTGCAGGCCCA
  CTGTAAGCTGGGTTTGACTGCGACCCTCGTCCGCGAAGATGACAAAATTGTGGATTTAAATTTTCTGATT
  GGGCCTAAGCTCTACGAAGCCAACTGGATGGAGCTGCAGAATAATGGCTACATCGCCAAAGTCCAGTGTG
  CTGAGGTCTGGTGCCCTATGTCTCCTGAATTTTACCGGGAATATGTGGCAATCAAAACCAAGAAACGAAT
  CTTGCTGTACACCATGAACCCCAACAAATTTAGAGCTTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGG
  AATGACAAGATTATTGTCTTTGCTGACAATGTGTTTGCCCTAAAGGAATATGCCATTCGACTGAACAAAC
  CCTATATCTACGGACCTACGTCTCAGGGGGAAAGGATGCAAATTCTCCAGAATTTCAAGCACAACCCCAA
  AATTAACACCATCTTCATATCCAAGGTAGGTGACACTTCGTTTGATCTGCCGGAAGCAAATGTCCTCATT
  CAGATCTCATCCCATGGTGGCTCCAGGCGTCAGGAAGCCCAAAGGCTAGGGCGGGTGCTTCGAGCTAAAA
  AAGGGATGGTTGCAGAAGAGTACAATGCCTTTTTCTACTCACTGGTATCCCAGGACACACAGGAAATGGC
  TTACTCAACCAAGCGGCAGAGATTCTTGGTAGATCAAGGTTATAGCTTCAAGGTGATCACGAAACTCGCT
  GGCATGGAGGAGGAAGACTTGGCGTTTTCGACAAAAGAAGAGCAACAGCAGCTCTTACAGAAAGTCCTGG
  CAGCCACTGACCTGGATGCCGAGGAGGAGGTGGTGGCTGGGGAATTTGGCTCCAGATCCAGCCAGGCATC
  TCGGCGCTTTGGCACCATGAGTTCTATGTCTGGGGCCGACGACACTGTGTACATGGAGTACCACTCATCG
  CGGAGCAAGGCGCCCAGCAAACATGTACACCCGCTCTTCAAGCGCTTTAGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239397 representing NM_001303416
  Red=Cloning site Green=Tags(s)
  
  MPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTS
  DITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEG
  EATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQ
  EMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGA
  GKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLG
  HTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLI
  GPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERR
  NDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLI
  QISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLA
  GMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSS
  RSKAPSKHVHPLFKRFRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303416
• ORF Size: 2154 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303416.1, NP_001290345.1
• RefSeq Size: 2865 bp
• RefSeq ORF: 2157 bp
• Locus ID: 2071
• Cytogenetics: 2q14.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair
• MW: 82.4 kDa
• Gene Summary: 'This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]'