XPB (ERCC3) (NM_001303416) Human Untagged Clone

CAT#: SC337291

ERCC3 (untagged) - Human excision repair cross-complementation group 3 (ERCC3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERCC3
• Synonyms: BTF2; GTF2H; RAD25; TFIIH; TTD2; XPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001303416, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGCTGAAGGACGACCACACCTCCAGGCCCCTCTGGGTGGCTCCCGATGGCCATATCTTCTTGGAAG
  CCTTCTCTCCAGTTTACAAATATGCCCAAGACTTCTTGGTGGCTATTGCAGAGCCAGTGTGCCGACCAAC
  CCATGTGCATGAGTACAAACTAACTGCCTACTCCTTGTATGCAGCTGTCAGCGTTGGGCTGCAAACCAGT
  GACATCACCGAGTACCTCAGGAAGCTCAGCAAGACTGGAGTCCCTGATGGAATTATGCAGTTTATTAAGT
  TGTGTACTGTCAGCTATGGAAAAGTCAAGCTGGTCTTGAAGCACAACAGATACTTCGTTGAAAGTTGCCA
  CCCTGATGTAATCCAGCATCTTCTCCAGGACCCCGTGATCCGAGAATGCCGCTTAAGAAACTCTGAAGGG
  GAGGCCACTGAGCTCATCACAGAGACTTTCACAAGCAAATCTGCCATTTCTAAGACTGCTGAAAGCAGTG
  GTGGGCCCTCCACTTCCCGAGTGACAGATCCACAGGGTAAATCTGACATCCCCATGGACCTGTTTGACTT
  CTATGAGCAAATGGACAAGGATGAAGAAGAAGAAGAAGAGACACAGACAGTGTCTTTTGAAGTCAAGCAG
  GAAATGATTGAGGAACTCCAGAAACGTTGCATCCACCTGGAGTACCCTCTGTTGGCAGAATATGACTTCC
  GGAATGATTCTGTCAACCCTGATATCAACATTGACCTAAAGCCCACAGCTGTCCTCAGACCCTATCAGGA
  GAAGAGCTTGCGAAAGATGTTTGGAAACGGGCGTGCACGTTCGGGGGTCATTGTTCTTCCCTGCGGTGCT
  GGAAAGTCCCTGGTTGGTGTGACTGCTGCATGCACTGTCAGAAAACGCTGTCTGGTGCTGGGCAACTCAG
  CTGTTTCTGTGGAGCAGTGGAAAGCCCAGTTCAAGATGTGGTCCACCATTGACGACAGCCAGATCTGCCG
  GTTCACCTCCGATGCCAAGGACAAGCCCATCGGCTGCTCCGTTGCCATTAGCACCTACTCCATGCTGGGC
  CACACCACCAAAAGGTCCTGGGAGGCCGAGCGAGTCATGGAGTGGCTCAAGACCCAGGAGTGGGGCCTCA
  TGATCCTGGATGAAGTGCACACCATACCAGCCAAGATGTTCCGAAGGGTGCTCACCATCGTGCAGGCCCA
  CTGTAAGCTGGGTTTGACTGCGACCCTCGTCCGCGAAGATGACAAAATTGTGGATTTAAATTTTCTGATT
  GGGCCTAAGCTCTACGAAGCCAACTGGATGGAGCTGCAGAATAATGGCTACATCGCCAAAGTCCAGTGTG
  CTGAGGTCTGGTGCCCTATGTCTCCTGAATTTTACCGGGAATATGTGGCAATCAAAACCAAGAAACGAAT
  CTTGCTGTACACCATGAACCCCAACAAATTTAGAGCTTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGG
  AATGACAAGATTATTGTCTTTGCTGACAATGTGTTTGCCCTAAAGGAATATGCCATTCGACTGAACAAAC
  CCTATATCTACGGACCTACGTCTCAGGGGGAAAGGATGCAAATTCTCCAGAATTTCAAGCACAACCCCAA
  AATTAACACCATCTTCATATCCAAGGTAGGTGACACTTCGTTTGATCTGCCGGAAGCAAATGTCCTCATT
  CAGATCTCATCCCATGGTGGCTCCAGGCGTCAGGAAGCCCAAAGGCTAGGGCGGGTGCTTCGAGCTAAAA
  AAGGGATGGTTGCAGAAGAGTACAATGCCTTTTTCTACTCACTGGTATCCCAGGACACACAGGAAATGGC
  TTACTCAACCAAGCGGCAGAGATTCTTGGTAGATCAAGGTTATAGCTTCAAGGTGATCACGAAACTCGCT
  GGCATGGAGGAGGAAGACTTGGCGTTTTCGACAAAAGAAGAGCAACAGCAGCTCTTACAGAAAGTCCTGG
  CAGCCACTGACCTGGATGCCGAGGAGGAGGTGGTGGCTGGGGAATTTGGCTCCAGATCCAGCCAGGCATC
  TCGGCGCTTTGGCACCATGAGTTCTATGTCTGGGGCCGACGACACTGTGTACATGGAGTACCACTCATCG
  CGGAGCAAGGCGCCCAGCAAACATGTACACCCGCTCTTCAAGCGCTTTAGGAAATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001303416
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303416.1, NP_001290345.1
• RefSeq Size: 2865 bp
• RefSeq ORF: 2157 bp
• Locus ID: 2071
• Cytogenetics: 2q14.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair
• Gene Summary: 'This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2 and 3 encodes the same isoform.