HSPC302 (TBCK) (NM_001290768) Human Tagged ORF Clone

CAT#: RC239411

TBCK (myc-DDK-tagged) - Human TBC1 domain containing kinase (TBCK), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBCK
• Synonyms: HSPC302; IHPRF3; TBCKL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239411 representing NM_001290768
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGTAAAAAACCATTGCCTTCTGGCCCCAAATCAGATGTATGGTCTCTTGGAATCATTTTATTTG
  AGCTTTGTGTGGGAAGAAAATTATTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACTTT
  GGATTGTGTAGATGACACTTTAATAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAGCTT
  CCTGAAACTGTGATAGATCTTTTGAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCAGATG
  AATTAATGAAGGACAAAGTATTCAGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCCAGTCT
  GTTTTCATCTTCTCTGAGATGTGCTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAAGATATA
  AATAATGATTACCTGGCAGAAAGATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGAGGTGACT
  TGGAGAAAGAGCTTGTCAACAAGGAAATCATTCGATCCAAACCACCTATCTGCACACTCCCCAATTTTCT
  CTTTGAGGATGGTGAAAGCTTTGGACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACCACTGTGACA
  TTGTCGTTATGCCAGCTAAGAAATAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCATTACTTGAAG
  ATGACCAGTCTAATTTACCTCATTCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACGCTCCCTTTAAT
  CATCAGAGAGAAGGATACAGAGTACCAACTAAATAGAATTATTCTCTTCGACAGGCTGCTAAAGGCTTAT
  CCATATAAAAAAAACCAAATCTGGAAAGAAGCAAGAGTTGACATTCCTCCTCTTATGAGAGGTTTAACCT
  GGGCTGCTCTTCTGGGAGTTGAGGGAGCTATTCATGCCAAGTACGATGCAATTGATAAAGACACTCCAAT
  TCCTACAGATAGACAAATTGAAGTGGATATTCCTCGCTGTCATCAGTACGATGAACTGTTATCATCACCA
  GAAGGTCATGCAAAATTTAGGCGTGTATTAAAAGCCTGGGTAGTGTCTCATCCTGATCTTGTGTATTGGC
  AAGGTCTTGACTCACTTTGTGCTCCATTCCTATATCTAAACTTCAATAATGAAGCCTTGGCTTATGCATG
  TATGTCTGCTTTTATTCCCAAATACCTGTATAACTTCTTCTTAAAAGACAACTCACATGTAATACAAGAG
  TATCTGACTGTCTTCTCTCAGATGATTGCATTTCATGATCCAGAGCTGAGTAATCATCTCAATGAGATTG
  GTTTCATTCCAGATCTCTATGCCATCCCTTGGTTTCTTACCATGTTTACTCATGTATTTCCACTACACAA
  AATTTTCCACCTCTGGGATACCTTACTACTTGGGAATTCCTCTTTCCCATTCTGTATTGGAGTAGCAATT
  CTTCAGCAGCTGCGGGACCGGCTTTTGGCTAATGGCTTTAATGAGTGTATTCTTCTCTTCTCCGATTTAC
  CAGAAATTGACATTGAACGCTGTGTGAGAGAATCTATCAACCTGTTTTGTTGGACTCCTAAAAGTGCTAC
  TTACAGACAGCATGCTCAACCTCCAAAGCCATCTTCTGACAGCAGTGGAGGCAGAAGTTCGGCACCTTAT
  TTCTCTGCTGAGTGTCCAGATCCTCCAAAGACAGATCTGTCAAGAGAATCCATCCCATTAAATGACCTGA
  AGTCAGAAGTATCACCACGGATTTCAGCAGAGGACCTGATTGACTTGTGTGAGCTCACAGTGACAGGCCA
  CTTCAAAACACCCAGCAAGAAAACAAAGTCCAGTAAACCAAAGCTCCTGGTGGTTGACATCCGGAATAGT
  GAAGACTTTATTCGTGGTCACATTTCAGGAAGCATCAACATTCCATTCAGTGCTGCCTTCACTGCAGAAG
  GGGAGCTTACCCAGGGCCCTTACACTGCTATGCTCCAGAACTTCAAAGGGAAGGTCATTGTCATCGTGGG
  GCATGTGGCAAAACACACAGCTGAGTTTGCAGCTCACCTTGTGAAGATGAAATATCCAAGAATCTGTATT
  CTAGATGGTGGCATTAATAAAATAAAGCCAACAGGCCTCCTCACCATCCCATCTCCTCAAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239411 representing NM_001290768
  Red=Cloning site Green=Tags(s)
  
  MPSKKPLPSGPKSDVWSLGIILFELCVGRKLFQSLDISERLKFLLTLDCVDDTLIVLAEEHGCLDIIKEL
  PETVIDLLNKCLTFHPSKRPTPDELMKDKVFSEVSPLYTPFTKPASLFSSSLRCADLTLPEDISQLCKDI
  NNDYLAERSIEEVYYLWCLAGGDLEKELVNKEIIRSKPPICTLPNFLFEDGESFGQGRDRSSLLDDTTVT
  LSLCQLRNRLKDVGGEAFYPLLEDDQSNLPHSNSNNELSAAATLPLIIREKDTEYQLNRIILFDRLLKAY
  PYKKNQIWKEARVDIPPLMRGLTWAALLGVEGAIHAKYDAIDKDTPIPTDRQIEVDIPRCHQYDELLSSP
  EGHAKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQE
  YLTVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAI
  LQQLRDRLLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPPKPSSDSSGGRSSAPY
  FSAECPDPPKTDLSRESIPLNDLKSEVSPRISAEDLIDLCELTVTGHFKTPSKKTKSSKPKLLVVDIRNS
  EDFIRGHISGSINIPFSAAFTAEGELTQGPYTAMLQNFKGKVIVIVGHVAKHTAEFAAHLVKMKYPRICI
  LDGGINKIKPTGLLTIPSPQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290768
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001290768.1, NP_001277697.1
• RefSeq Size: 5410
• RefSeq ORF: 2166
• Locus ID: 93627
• Protein Families: Druggable Genome, Protein Kinase
• MW: 81.8 kDa
• Gene Summary: This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]