STAT5A (NM_001288720) Human Tagged ORF Clone

CAT#: RC239510

STAT5A (myc-DDK-tagged) - Human signal transducer and activator of transcription 5A (STAT5A), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STAT5A
• Synonyms: MGF; STAT5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239510 representing NM_001288720
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCTGGATCCAGGCCCAGCAGCTGCAGGGAGACGCGCTGCGCCAGATGCAGGTGCTGTACGGCC
  AGCACTTCCCCATCGAGGTCCGGCACTACTTGGCCCAGTGGATTGAGAGCCAGCCATGGGATGCCATTGA
  CTTGGACAATCCCCAGGACAGAGCCCAAGCCACCCAGCTCCTGGAGGGCCTGGTGCAGGAGCTGCAGAAG
  AAGGCGGAGCACCAGGTGGGGGAAGATGGGTTTTTACTGAAGATCAAGCTGGGGCACTACGCCACGCAGC
  TCCAGAAAACATATGACCGCTGCCCCCTGGAGCTGGTCCGCTGCATCCGGCACATTCTGTACAATGAACA
  GAGGCTGGTCCGAGAAGCCAACAATTGCAGCTCTCCGGCTGGGATCCTGGTTGACGCCATGTCCCAGAAG
  CACCTTCAGATCAACCAGACATTTGAGGAGCTGCGACTGGTCACGCAGGACACAGAGAATGAGCTGAAGA
  AACTGCAGCAGACTCAGGAGTACTTCATCATCCAGTACCAGGAGAGCCTGAGGATCCAAGCTCAGTTTGC
  CCAGCTGGCCCAGCTGAGCCCCCAGGAGCGTCTGAGCCGGGAGACGGCCCTCCAGCAGAAGCAGGTGTCT
  CTGGAGGCCTGGTTGCAGCGTGAGGCACAGACACTGCAGCAGTACCGCGTGGAGCTGGCCGAGAAGCACC
  AGAAGACCCTGCAGCTGCTGCGGAAGCAGCAGACCATCATCCTGGATGACGAGCTGATCCAGTGGAAGCG
  GCGGCAGCAGCTGGCCGGGAACGGCGGGCCCCCCGAGGGCAGCCTGGACGTGCTACAGTCCTGGTGTGAG
  AAGTTGGCCGAGATCATCTGGCAGAACCGGCAGCAGATCCGCAGGGCTGAGCACCTCTGCCAGCAGCTGC
  CCATCCCCGGCCCAGTGGAGGAGATGCTGGCCGAGGTCAACGCCACCATCACGGACATTATCTCAGCCCT
  GGTGACCAGCACATTCATCATTGAGAAGCAGCCTCCTCAGGTCCTGAAGACCCAGACCAAGTTTGCAGCC
  ACCGTACGCCTGCTGGTGGGCGGGAAGCTGAACGTGCACATGAATCCCCCCCAGGTGAAGGCCACCATCA
  TCAGTGAGCAGCAGGCCAAGTCTCTGCTTAAAAATGAGAACACCCGCAACGAGTGCAGTGGTGAGATCCT
  GAACAACTGCTGCGTGATGGAGTACCACCAAGCCACGGGCACCCTCAGTGCCCACTTCAGGAACATGTCA
  CTGAAGAGGATCAAGCGTGCTGACCGGCGGGGTGCAGAGTCCGTGACAGAGGAGAAGTTCACAGTCCTGT
  TTGAGTCTCAGTTCAGTGTTGGCAGCAATGAGCTTGTGTTCCAGGTGAAGGGCAGGGTGCCATTTGCCGT
  GCCTGACAAAGTGCTGTGGCCGCAGCTGTGTGAGGCGCTCAACATGAAATTCAAGGCCGAAGTGCAGAGC
  AACCGGGGCCTGACCAAGGAGAACCTCGTGTTCCTGGCGCAGAAACTGTTCAACAACAGCAGCAGCCACC
  TGGAGGACTACAGTGGCCTGTCCGTGTCCTGGTCCCAGTTCAACAGGGAGAACTTGCCGGGCTGGAACTA
  CACCTTCTGGCAGTGGTTTGACGGGGTGATGGAGGTGTTGAAGAAGCACCACAAGCCCCACTGGAATGAT
  GGGGCCATCCTAGGTTTTGTGAATAAGCAACAGGCCCACGACCTGCTCATCAACAAGCCCGACGGGACCT
  TCTTGTTGCGCTTTAGTGACTCAGAAATCGGGGGCATCACCATCGCCTGGAAGTTTGACTCCCCGGAACG
  CAACCTGTGGAACCTGAAACCATTCACCACGCGGGATTTCTCCATCAGGTCCCTGGCTGACCGGCTGGGG
  GACCTGAGCTATCTCATCTATGTGTTTCCTGACCGCCCCAAGGATGAGGTCTTCTCCAAGTACTACACTC
  CTGTGCTGGCTAAAGCTGTTGATGGATATGTGAAACCACAGATCAAGCAAGTGGTCCCTGAGTTTGTGAA
  TGCATCTGCAGATGCTGGGGGCAGCAGCGCCACGTACATGGACCAGGCCCCCTCCCCAGCTGTGTGCCCC
  CAGGCTCCCTATAACATGTACCCACAGAACCCTGACCATGTACTCGATCAGGATGGAGAATTCGACCTGG
  ATGAGACCATGGATGTGGCCAGGCACGTGGAGGAACTCTTACGCCGACCAATGGACAGTCTTGACTCCCG
  CCTCTCGCCCCCTGCCGGTCTTTTCACCTCTGCCAGAGGCTCCCTCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239510 representing NM_001288720
  Red=Cloning site Green=Tags(s)
  
  MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQK
  KAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLELVRCIRHILYNEQRLVREANNCSSPAGILVDAMSQK
  HLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLSPQERLSRETALQQKQVS
  LEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCE
  KLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAA
  TVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSAHFRNMS
  LKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKGRVPFAVPDKVLWPQLCEALNMKFKAEVQS
  NRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGWNYTFWQWFDGVMEVLKKHHKPHWND
  GAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFSIRSLADRLG
  DLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCP
  QAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFTSARGSLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288720
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001288720.1, NP_001275649.1
• RefSeq Size: 4132
• RefSeq ORF: 2292
• Locus ID: 6776
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Chronic myeloid leukemia, ErbB signaling pathway, Jak-STAT signaling pathway, Pathways in cancer
• MW: 87.8 kDa
• Gene Summary: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]