CDC2L1 (CDK11B) (NM_001291345) Human Tagged ORF Clone

CAT#: RC239535

CDK11B (myc-DDK-tagged) - Human cyclin-dependent kinase 11B (CDK11B), transcript variant 10

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDK11B
• Synonyms: CDC2L1; CDK11; CDK11-p46; CDK11-p58; CDK11-p110; CLK-1; p58; p58CDC2L1; p58CLK-1; PITSLREA; PK58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239535 representing NM_001291345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGA
  AGGAACAAGAGGAGAAAGCAGAGATAAAACGCTTAAAAAATTCTGATGACCGGGATTCCAAGCGGGATTC
  CCTTGAGGAGGGGGAGCTGAGAGATCACCGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAGAGAA
  GACTCTATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGA
  AAGAAAAAGCTCATCACAGAAAAGATGAAAAGAGAAAAGAGAAACGGAAGCATGCTAGAGTGAAAGAAAA
  AGAAAGAGAGCACGAACGTCGGAAACGGCATCGAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGA
  CAGAAGAGAAGGGAGATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTTAGAAAGGA
  AGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCG
  GGCAGAGGAGCGGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAG
  GACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGCCGCCGCGGGAGCGGTTCG
  AGTTGGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACA
  GGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCT
  GAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGG
  AGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGA
  AGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCA
  GAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGC
  AGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTCGCCCATCGAGCTCAAGCA
  GGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGG
  ATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAA
  AGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCGCTGAGGGAGATCAACACCATCCT
  CAAGGCCCAGCATCCCAACATCGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTAC
  ATCGTGATGAACTATGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAG
  GGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGTGGGGTGAAACACCTGCACGACAACTGGATCCTGCA
  CCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGACTTCGGGCTG
  GCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCTGTGGTACCGCGCCCCAG
  AGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGTTGCATCTTCGGGGA
  GCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAAGTCAGAAATCGATCAGATCAACAAGGTGTTCAAGGAT
  CTGGGGACCCCTAGTGAGAAAATCTGGCCCGGCTACAGCGAGCTCCCAGCAGTCAAGAAGATGACCTTCA
  GCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCAT
  GAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTC
  CGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTG
  TGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCT
  GAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTC
  AAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239535 representing NM_001291345
  Red=Cloning site Green=Tags(s)
  
  MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHRMEITIRNSPYRRE
  DSMEDRGEEDDSLAIKPPQQMSRKEKAHHRKDEKRKEKRKHARVKEKEREHERRKRHREEQDKARREWER
  QKRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMRE
  DYSDKVKASHWSRSPPRPPRERFELGDGRKPVKEEKMEERDLLSDLQDISDSERKTSSAESSSAESGSGS
  EEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEERENENHLLVVP
  ESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALSPIELKQELPKYLPALQGCRSVEEFQCLNR
  IEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIY
  IVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGL
  AREYGSPLKAYTPVVVTLWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEIDQINKVFKD
  LGTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYF
  RETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSL
  KF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291345
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291345.1, NP_001278274.1
• RefSeq Size: 3072 bp
• RefSeq ORF: 2319 bp
• Locus ID: 984
• Cytogenetics: 1p36.33
• Protein Families: Druggable Genome, Transcription Factors
• MW: 90.7 kDa
• Gene Summary: 'This gene encodes a member of the serine/threonine protein kinase family. Members of this kinase family are known to be essential for eukaryotic cell cycle control. Due to a segmental duplication, this gene shares very high sequence identity with a neighboring gene. These two genes are frequently deleted or altered in neuroblastoma. The protein kinase encoded by this gene can be cleaved by caspases and may play a role in cell apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]'