HERC4 (NM_001278186) Human Tagged ORF Clone

CAT#: RC239582

HERC4 (myc-DDK-tagged) - Human HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HERC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239582 representing NM_001278186
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAAATGGAAGGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTA
  CCAGTCATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATTGCTTGTGG
  ACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGGCTTGGTGGTAATGGG
  CAGCTGGGAACCGGTTCAACAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGGTACCCCTATA
  ATGGGCAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAAAAGAATTTTCTCAGGGGGAGA
  TCAAAGCTTTTCACATTACTCTAGTCCCCAGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCG
  ACAAAGCAGATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTCTGGAAGGTTTC
  CTGTGGAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAATGGAAGTTTTTTAGC
  TGTTAGCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAATGCTGCTAGGCTT
  TTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGA
  ATCTTATTCCTAAACTGACTAGCTCCTTACCTGATGTTGAAGCATTGAGGTTTTATCTTACTCTACCAGA
  ATGTCCCCTGATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTTGGTACAGCTCTTGTGAAC
  CTAGAAAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGA
  TAGTAGAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCTTCTGA
  AAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAAATACTACATAGGGTAAATGAG
  AAAATGGGACAGATTATACAGTATGATAAATTTTATATACATGAAGTACAAGAATTGATAGACATAAGAA
  ATGATTATATCAACTGGGTCCAACAGCAGGCCTATGGAATGTTGGCAGATATCCCTGTTACAATCTGTAC
  ATATCCATTTGTATTTGATGCCCAAGCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGATGCAG
  ATGGCTATTGATCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAATC
  CCTGCTTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAAACAAA
  GAACATAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTGGATGCAGGAGGGGTG
  CGCAAAGAATTTTTCTTGCTCATCATGAGGGAATTATTGGATCCTAAATACGGCATGTTTAGGTATTATG
  AAGATTCCAGGCTCATTTGGTTTTCTGATAAGACATTTGAAGACAGTGATTTGTTCCATTTGATTGGTGT
  TATCTGTGGCTTAGCAATTTATAATTGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAAA
  CTACTGAAAAAGAAGCCATCCTTGGATGATTTGAAAGAACTAATGCCTGATGTTGGGAGAAGCATGCAAC
  AGTTACTGGATTATCCAGAAGATGACATAGAGGAAACATTTTGTCTTAATTTTACGATCACAGTTGAAAA
  CTTTGGTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTTAACAAACAAAATCGG
  CAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAATAAATCAGTGGCTTCCTTATTTGATGCTTTTC
  ATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTCCTTCTGCTCTTTCAGCCTAATGAACTACAAGCAAT
  GGTCATTGGAAATACAAATTATGATTGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGG
  GCAGAACATCCTACGATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGT
  TTCTGTTATTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCA
  GTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTTCTGGATCTTCCAAAA
  TATACAGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAA
  TA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239582 representing NM_001278186
  Red=Cloning site Green=Tags(s)
  
  MMKMEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNG
  QLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNP
  TKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARL
  LFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVN
  LEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNE
  KMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQ
  MAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGV
  RKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKK
  LLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNR
  QEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYW
  AEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPK
  YTEKETLRSKLIQAIDHNEGFSLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278186
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001278186.1, NP_001265115.1
• RefSeq Size: 4255
• RefSeq ORF: 2385
• Locus ID: 26091
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 90.6 kDa
• Gene Summary: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]). [supplied by OMIM, Mar 2008]