ZNF43 (NM_001256653) Human Tagged ORF Clone

CAT#: RC239625

ZNF43 (myc-DDK-tagged) - Human zinc finger protein 43 (ZNF43), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF43
• Synonyms: HTF6; KOX27; ZNF39L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239625 representing NM_001256653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGACATCCTGGAAGCTGGGAAATGGGACCATTGACATTTATGGATGTGGCCATAGAATTCTGTC
  TGGAGGAGTGGCAATGCCTGGACATTGCACAGCAGAATTTATATAGGAATGTGATGTTAGAGAACTACAG
  AAACCTGGTCTTCCTGGGTATTGCTGTCTCTAAGCCAGACCTGATCACCTGTCTGGAGCAAGAAAAAGAG
  CCTTGGGAGCCTATGAGGAGACATGAAATGGTAGCCAAACCCCCAGTTATGTGTTCTCATTTTACCCAAG
  ACTTTTGGCCAGAGCAGCATATAAAAGATCCTTTCCAAAAAGCGACACTGAGAAGATATAAAAACTGTGA
  ACATAAAAATGTACATTTAAAAAAAGACCATAAAAGTGTGGATGAGTGTAAGGTGCACAGAGGAGGTTAT
  AATGGATTTAACCAATGTTTGCCAGCTACCCAGAGCAAAATATTTCTATTTGATAAATGTGTGAAAGCCT
  TTCATAAATTTTCAAATTCAAACAGACATAAGATAAGCCATACTGAAAAAAAACTTTTCAAATGCAAAGA
  ATGTGGCAAATCATTTTGCATGCTTCCACATCTAGCTCAACATAAAATAATTCATACCAGAGTGAATTTC
  TGCAAATGTGAAAAATGTGGAAAAGCTTTTAACTGCCCTTCAATCATCACTAAACATAAGAGAATTAATA
  CTGGAGAGAAACCCTACACATGTGAAGAATGTGGCAAAGTCTTTAATTGGTCCTCACGCCTTACTACACA
  TAAAAAAAATTATACTAGATACAAACTCTACAAATGTGAAGAATGTGGCAAAGCTTTTAACAAGTCCTCA
  ATCCTTACTACCCATAAGATAATTCGCACTGGAGAGAAATTCTACAAATGTAAAGAATGTGCCAAAGCTT
  TTAACCAATCCTCAAACCTTACTGAACATAAGAAAATTCATCCTGGAGAGAAACCTTACAAATGTGAAGA
  ATGTGGCAAAGCCTTTAACTGGCCCTCAACTCTTACTAAACATAAGAGAATTCATACTGGAGAGAAACCC
  TACACATGTGAAGAATGTGGCAAAGCCTTTAACCAGTTCTCAAACCTTACTACACATAAGAGAATCCATA
  CTGCAGAGAAATTCTATAAATGTACAGAATGTGGTGAAGCTTTTAGCCGGTCCTCAAACCTTACTAAACA
  TAAGAAAATTCATACTGAAAAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAGTGGTCCTCA
  AAGCTTACTGAACATAAGTTAACTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCT
  TTAACTGGCCCTCAACCCTTACTAAACATAACAGAATTCATACTGGAGAGAAACCCTACAAATGTGAAGT
  ATGTGGCAAAGCCTTTAACCAGTTCTCAAACCTTACTACACATAAGAGAATTCATACTGCAGAAAAACCG
  TACAAATGTGAAGAATGTGGCAAAGCTTTTAGCCGGTCCTCAAACCTTACTAAACATAAGAAAATTCACA
  TTGAAAAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAGTGGTCCTCAAAGCTTACTGAACA
  TAAGATAACTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAACCATTTCTCA
  ATCCTTACCAAACATAAGAGGATTCATACTGGAGAGAAACCCTACAAGTGTGAAGAATGTGGCAAAGCTT
  TTACCCAATCCTCAAACCTTACTACACATAAGAAAATTCATACTGGAGAGAAATTCTACAAATGTGAAGA
  ATGTGGCAAAGCTTTTACCCAATCTTCAAACCTTACTACACATAAAAAAATTCATACTGGAGGAAAACCC
  TACAAATGTGAAGAATGTGGCAAAGCTTTTAACCAGTTCTCAACTCTTACTAAACATAAGATAATTCACA
  CTGAGGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCTTTAAGTGGTCCTCAACCCTTACTAAACA
  TAAGATAATTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAACTGTCCTCA
  ACCCTTTCTACACATAAGATTATTCATACTGGAGAGAAACCCTACAAATGTGAAAAATGTGGCAAAGCTT
  TTAACCGATCCTCAAACCTTATTGAACATAAGAAAATTCATACTGGAGAGCAACCCTACAAATGTGAAGA
  ATGTGGCAAAGCATTTAACTATTCCTCACACCTTAATACACATAAGAGAATTCATACTAAAGAGCAACCC
  TACAAATGTAAAGAATGTGGCAAAGCTTTCAACCAATATTCAAACCTTACTACACATAACAAAATTCATA
  CTGGAGAGAAACTCTACAAACCTGAAGATGTGACAGTGATTTTGACAACACCTCAAACTTTTTCAAACAT
  AAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239625 representing NM_001256653
  Red=Cloning site Green=Tags(s)
  
  MPGHPGSWEMGPLTFMDVAIEFCLEEWQCLDIAQQNLYRNVMLENYRNLVFLGIAVSKPDLITCLEQEKE
  PWEPMRRHEMVAKPPVMCSHFTQDFWPEQHIKDPFQKATLRRYKNCEHKNVHLKKDHKSVDECKVHRGGY
  NGFNQCLPATQSKIFLFDKCVKAFHKFSNSNRHKISHTEKKLFKCKECGKSFCMLPHLAQHKIIHTRVNF
  CKCEKCGKAFNCPSIITKHKRINTGEKPYTCEECGKVFNWSSRLTTHKKNYTRYKLYKCEECGKAFNKSS
  ILTTHKIIRTGEKFYKCKECAKAFNQSSNLTEHKKIHPGEKPYKCEECGKAFNWPSTLTKHKRIHTGEKP
  YTCEECGKAFNQFSNLTTHKRIHTAEKFYKCTECGEAFSRSSNLTKHKKIHTEKKPYKCEECGKAFKWSS
  KLTEHKLTHTGEKPYKCEECGKAFNWPSTLTKHNRIHTGEKPYKCEVCGKAFNQFSNLTTHKRIHTAEKP
  YKCEECGKAFSRSSNLTKHKKIHIEKKPYKCEECGKAFKWSSKLTEHKITHTGEKPYKCEECGKAFNHFS
  ILTKHKRIHTGEKPYKCEECGKAFTQSSNLTTHKKIHTGEKFYKCEECGKAFTQSSNLTTHKKIHTGGKP
  YKCEECGKAFNQFSTLTKHKIIHTEEKPYKCEECGKAFKWSSTLTKHKIIHTGEKPYKCEECGKAFKLSS
  TLSTHKIIHTGEKPYKCEKCGKAFNRSSNLIEHKKIHTGEQPYKCEECGKAFNYSSHLNTHKRIHTKEQP
  YKCKECGKAFNQYSNLTTHNKIHTGEKLYKPEDVTVILTTPQTFSNIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256653
• ORF Size: 2454 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256653.1, NP_001243582.1
• RefSeq Size: 5249 bp
• RefSeq ORF: 2457 bp
• Locus ID: 7594
• Cytogenetics: 19p12
• Protein Families: Transcription Factors
• MW: 95.6 kDa
• Gene Summary: 'This gene belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene regulation and development, and are quite conserved throughout evolution. Like this gene product, a third of the zinc finger proteins containing C2H2 fingers also contain the KRAB domain, which has been found to be involved in protein-protein interactions. [provided by RefSeq, Jul 2008]'