PDE3A (NM_001244683) Human Tagged ORF Clone

CAT#: RC239628

PDE3A (myc-DDK-tagged) - Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE3A
• Synonyms: CGI-PDE; CGI-PDE-A; CGI-PDE A; HTNB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239628 representing NM_001244683
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACGATATTTTCCAAATCGTGGAGCTTCTACTGGGAAAAGTCTTCAGGAACCAGTATTACTGTGG
  ACATCGCCGTCATGGGCGAGGCCCACGGCCTCATTACCGACCTCCTGGCAGACCCTTCTCTTCCACCAAA
  CGTGTGCACATCCTTGAGAGCCGTGAGCAACTTGCTCAGCACACAGCTCACCTTCCAGGCCATTCACAAG
  CCCAGAGTGAATCCCGTCACTTCGCTCAGTGAAAACTATACCTGTTCTGACTCTGAAGAGAGCTCTGAAA
  AAGACAAGCTTGCTATTCCAAAGCGCCTGAGAAGGAGTTTGCCTCCTGGCTTGTTGAGACGAGTTTCTTC
  CACTTGGACCACCACCACCTCGGCCACAGGTCTACCCACCTTGGAGCCTGCACCAGTACGGAGAGACCGC
  AGCACCAGCATCAAACTGCAGGAAGCACCTTCATCCAGTCCTGATTCTTGGAATAATCCAGTGATGATGA
  CCCTCACCAAAAGCAGATCCTTTACTTCATCCTATGCTATTTCTGCAGCTAACCATGTAAAGGCTAAAAA
  GCAAAGTCGACCAGGTGCCCTCGCTAAAATTTCACCTCTTTCATCGCCCTGCTCCTCACCTCTCCAAGGG
  ACTCCTGCCAGCAGCCTGGTCAGCAAAATTTCTGCAGTGCAGTTTCCAGAATCTGCTGACACAACTGCCA
  AACAAAGCCTAGGTTCTCACAGGGCCTTAACTTACACTCAGAGTGCCCCAGACCTATCCCCTCAAATCCT
  GACTCCACCTGTTATATGTAGCAGCTGTGGCAGACCATATTCCCAAGGGAATCCTGCTGATGAGCCCCTG
  GAGAGAAGTGGGGTAGCCACTCGGACACCAAGTAGAACAGATGACACTGCTCAAGTTACCTCTGATTATG
  AAACCAATAACAACAGTGACAGCAGTGACATTGTACAGAATGAAGATGAAACAGAGTGCCTGAGAGAGCC
  TCTGAGGAAAGCATCGGCTTGCAGCACCTATGCTCCTGAGACCATGATGTTTCTGGACAAACCAATTCTT
  GCTCCCGAACCTCTTGTCATGGATAACCTGGACTCAATTATGGAGCAGCTAAATACTTGGAATTTTCCAA
  TTTTTGATTTAGTGGAAAATATAGGAAGAAAATGTGGCCGTATTCTTAGTCAGGTATCTTACAGACTTTT
  TGAAGACATGGGCCTCTTTGAAGCTTTTAAAATTCCAATTAGGGAATTTATGAATTATTTTCATGCTTTG
  GAGATTGGATATAGGGATATTCCTTATCATAACAGAATCCATGCCACTGATGTTTTACATGCTGTTTGGT
  ATCTTACTACACAGCCTATTCCAGGCCTCTCAACTGTGATTAATGATCATGGTTCAACCAGTGATTCAGA
  TTCTGACAGTGGATTTACACATGGACATATGGGATATGTATTCTCAAAAACGTATAATGTGACAGATGAT
  AAATACGGATGTCTGTCTGGGAATATCCCTGCCTTGGAGTTGATGGCGCTGTATGTGGCTGCAGCCATGC
  ACGATTATGATCATCCAGGAAGGACTAATGCTTTCCTGGTTGCAACTAGTGCTCCTCAGGCGGTGCTATA
  TAACGATCGTTCAGTTTTGGAGAATCATCACGCAGCTGCTGCATGGAATCTTTTCATGTCCCGGCCAGAG
  TATAACTTCTTAATTAACCTTGACCATGTGGAATTTAAGCATTTCCGTTTCCTTGTCATTGAAGCAATTT
  TGGCCACTGACCTGAAGAAACACTTTGACTTCGTAGCCAAATTTAATGGCAAGGTAAATGATGATGTTGG
  AATAGATTGGACCAATGAAAATGATCGTCTACTGGTTTGTCAAATGTGTATAAAGTTGGCTGATATCAAT
  GGTCCAGCTAAATGTAAAGAACTCCATCTTCAGTGGACAGATGGTATTGTCAATGAATTTTATGAACAGG
  GTGATGAAGAGGCCAGCCTTGGATTACCCATAAGCCCCTTCATGGATCGTTCTGCTCCTCAGCTGGCCAA
  CCTTCAGGAATCCTTCATCTCTCACATTGTGGGGCCTCTGTGCAACTCCTATGATTCAGCAGGACTAATG
  CCTGGAAAATGGGTGGAAGACAGCGATGAGTCAGGAGATACTGATGACCCAGAAGAAGAGGAGGAAGAAG
  CACCAGCACCAAATGAAGAGGAAACCTGTGAAAATAATGAATCTCCAAAAAAGAAGACTTTCAAAAGGAG
  AAAAATCTACTGCCAAATAACTCAGCACCTCTTACAGAACCACAAGATGTGGAAGAAAGTCATTGAAGAG
  GAGCAACGGTTGGCAGGCATAGAAAATCAATCCCTGGACCAGACCCCTCAGTCGCACTCTTCAGAACAGA
  TCCAGGCTATCAAGGAAGAAGAAGAAGAGAAAGGGAAACCAAGAGGCGAGGAGATACCAACCCAAAAGCC
  AGACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239628 representing NM_001244683
  Red=Cloning site Green=Tags(s)
  
  MVTIFSKSWSFYWEKSSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHK
  PRVNPVTSLSENYTCSDSEESSEKDKLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDR
  STSIKLQEAPSSSPDSWNNPVMMTLTKSRSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQG
  TPASSLVSKISAVQFPESADTTAKQSLGSHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPL
  ERSGVATRTPSRTDDTAQVTSDYETNNNSDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPIL
  APEPLVMDNLDSIMEQLNTWNFPIFDLVENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHAL
  EIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDD
  KYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPE
  YNFLINLDHVEFKHFRFLVIEAILATDLKKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADIN
  GPAKCKELHLQWTDGIVNEFYEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLM
  PGKWVEDSDESGDTDDPEEEEEEAPAPNEEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEE
  EQRLAGIENQSLDQTPQSHSSEQIQAIKEEEEEKGKPRGEEIPTQKPDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001244683
• ORF Size: 2457 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244683.1, NP_001231612.1
• RefSeq Size: 6576 bp
• RefSeq ORF: 2460 bp
• Locus ID: 5139
• Cytogenetics: 12p12.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Insulin signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 91.7 kDa
• Gene Summary: 'This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]'