PDE3A (NM_001244683) Human Untagged Clone

CAT#: SC337522

PDE3A (untagged) - Human phosphodiesterase 3A, cGMP-inhibited (PDE3A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE3A
• Synonyms: CGI-PDE; CGI-PDE-A; CGI-PDE A; HTNB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001244683, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGACGATATTTTCCAAATCGTGGAGCTTCTACTGGGAAAAGTCTTCAGGAACCAGTATTACTGTGG
  ACATCGCCGTCATGGGCGAGGCCCACGGCCTCATTACCGACCTCCTGGCAGACCCTTCTCTTCCACCAAA
  CGTGTGCACATCCTTGAGAGCCGTGAGCAACTTGCTCAGCACACAGCTCACCTTCCAGGCCATTCACAAG
  CCCAGAGTGAATCCCGTCACTTCGCTCAGTGAAAACTATACCTGTTCTGACTCTGAAGAGAGCTCTGAAA
  AAGACAAGCTTGCTATTCCAAAGCGCCTGAGAAGGAGTTTGCCTCCTGGCTTGTTGAGACGAGTTTCTTC
  CACTTGGACCACCACCACCTCGGCCACAGGTCTACCCACCTTGGAGCCTGCACCAGTACGGAGAGACCGC
  AGCACCAGCATCAAACTGCAGGAAGCACCTTCATCCAGTCCTGATTCTTGGAATAATCCAGTGATGATGA
  CCCTCACCAAAAGCAGATCCTTTACTTCATCCTATGCTATTTCTGCAGCTAACCATGTAAAGGCTAAAAA
  GCAAAGTCGACCAGGTGCCCTCGCTAAAATTTCACCTCTTTCATCGCCCTGCTCCTCACCTCTCCAAGGG
  ACTCCTGCCAGCAGCCTGGTCAGCAAAATTTCTGCAGTGCAGTTTCCAGAATCTGCTGACACAACTGCCA
  AACAAAGCCTAGGTTCTCACAGGGCCTTAACTTACACTCAGAGTGCCCCAGACCTATCCCCTCAAATCCT
  GACTCCACCTGTTATATGTAGCAGCTGTGGCAGACCATATTCCCAAGGGAATCCTGCTGATGAGCCCCTG
  GAGAGAAGTGGGGTAGCCACTCGGACACCAAGTAGAACAGATGACACTGCTCAAGTTACCTCTGATTATG
  AAACCAATAACAACAGTGACAGCAGTGACATTGTACAGAATGAAGATGAAACAGAGTGCCTGAGAGAGCC
  TCTGAGGAAAGCATCGGCTTGCAGCACCTATGCTCCTGAGACCATGATGTTTCTGGACAAACCAATTCTT
  GCTCCCGAACCTCTTGTCATGGATAACCTGGACTCAATTATGGAGCAGCTAAATACTTGGAATTTTCCAA
  TTTTTGATTTAGTGGAAAATATAGGAAGAAAATGTGGCCGTATTCTTAGTCAGGTATCTTACAGACTTTT
  TGAAGACATGGGCCTCTTTGAAGCTTTTAAAATTCCAATTAGGGAATTTATGAATTATTTTCATGCTTTG
  GAGATTGGATATAGGGATATTCCTTATCATAACAGAATCCATGCCACTGATGTTTTACATGCTGTTTGGT
  ATCTTACTACACAGCCTATTCCAGGCCTCTCAACTGTGATTAATGATCATGGTTCAACCAGTGATTCAGA
  TTCTGACAGTGGATTTACACATGGACATATGGGATATGTATTCTCAAAAACGTATAATGTGACAGATGAT
  AAATACGGATGTCTGTCTGGGAATATCCCTGCCTTGGAGTTGATGGCGCTGTATGTGGCTGCAGCCATGC
  ACGATTATGATCATCCAGGAAGGACTAATGCTTTCCTGGTTGCAACTAGTGCTCCTCAGGCGGTGCTATA
  TAACGATCGTTCAGTTTTGGAGAATCATCACGCAGCTGCTGCATGGAATCTTTTCATGTCCCGGCCAGAG
  TATAACTTCTTAATTAACCTTGACCATGTGGAATTTAAGCATTTCCGTTTCCTTGTCATTGAAGCAATTT
  TGGCCACTGACCTGAAGAAACACTTTGACTTCGTAGCCAAATTTAATGGCAAGGTAAATGATGATGTTGG
  AATAGATTGGACCAATGAAAATGATCGTCTACTGGTTTGTCAAATGTGTATAAAGTTGGCTGATATCAAT
  GGTCCAGCTAAATGTAAAGAACTCCATCTTCAGTGGACAGATGGTATTGTCAATGAATTTTATGAACAGG
  GTGATGAAGAGGCCAGCCTTGGATTACCCATAAGCCCCTTCATGGATCGTTCTGCTCCTCAGCTGGCCAA
  CCTTCAGGAATCCTTCATCTCTCACATTGTGGGGCCTCTGTGCAACTCCTATGATTCAGCAGGACTAATG
  CCTGGAAAATGGGTGGAAGACAGCGATGAGTCAGGAGATACTGATGACCCAGAAGAAGAGGAGGAAGAAG
  CACCAGCACCAAATGAAGAGGAAACCTGTGAAAATAATGAATCTCCAAAAAAGAAGACTTTCAAAAGGAG
  AAAAATCTACTGCCAAATAACTCAGCACCTCTTACAGAACCACAAGATGTGGAAGAAAGTCATTGAAGAG
  GAGCAACGGTTGGCAGGCATAGAAAATCAATCCCTGGACCAGACCCCTCAGTCGCACTCTTCAGAACAGA
  TCCAGGCTATCAAGGAAGAAGAAGAAGAGAAAGGGAAACCAAGAGGCGAGGAGATACCAACCCAAAAGCC
  AGACCAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001244683
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244683.1, NP_001231612.1
• RefSeq Size: 6576 bp
• RefSeq ORF: 2460 bp
• Locus ID: 5139
• Cytogenetics: 12p12.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Insulin signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.