BRD8 (NM_001300961) Human Tagged ORF Clone

CAT#: RC239701

BRD8 (myc-DDK-tagged) - Human bromodomain containing 8 (BRD8), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRD8
• Synonyms: p120; SMAP; SMAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239701 representing NM_001300961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCATCCGAGAGAAGCTATGTTTAGCATCTTCTGTCATGAGAAGTGGCGATCAAAATTGGTAAGGG
  TATCAGTTAGCAGAGCAATCAAGCCCTTTGCAGAACCTGGCCGCCCTCCAGACTGGTTCTCTCAAAAACA
  TTGTGCTTCCCAGTACTCGGAGCTTTTAGAGACCACTGAGACACCAAAACGGAAACGAGGTGAAAAGGGA
  GAAGTGGTGGAAACTGTTGAAGATGTTATTGTTCGGAAATTGACTGCTGAGCGAGTTGAAGAACTAAAGA
  AAGTGATAAAGGAAACCCAGGAGAGATATAGACGGCTAAAGAGAGATGCAGAACTAATTCAAGCTGGACA
  CATGGACAGCAGACTGGATGAGCTTTGCAATGACATTGCAACGAAAAAGAAATTGGAAGAAGAGGAGGCT
  GAAGTAAAGAGGAAGGCTACAGATGCTGCATACCAGGCTCGTCAAGCAGTAAAAACACCCCCCCGGAGGT
  TACCCACTGTGATGGTTCGCTCTCCTATAGATTCTGCCTCCCCAGGAGGTGATTATCCACTTGGGGACTT
  GACTCCAACCACTATGGAAGAGGCTACCTCTGGGGTCAATGAGAGTGAAATGGCTGTGGCTTCTGGCCAC
  CTGAACAGTACAGGTGTCCTCCTGGAGGTAGGCGGGGTCCTTCCCATGATACATGGTGGGGAGATACAGC
  AAACACCCAATACTGTTGCAGCCTCCCCTGCTGCATCAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGC
  TGGTCCTACACAGTTCACCACACCTCTTGCTTCCTTCACTACTGTTGCCAGTGAGCCTCCAGTTAAACTT
  GTGCCACCCCCTGTAGAGTCTGTGTCCCAAGCTACCATTGTCATGATGCCTGCGCTGCCAGCACCATCCT
  CTGCTCCGGCTGTCTCCACTACTGAAAGTGTAGCTCCAGTGAGTCAACCCGACAACTGTGTTCCCATGGA
  GGCTGTGGGGGATCCACATACTGTGACTGTTTCCATGGACAGCAGTGAAATATCCATGATCATCAATTCT
  ATCAAAGAAGAGTGTTTTCGATCAGGGGTAGCAGAGGCTCCTGTTGGATCAAAGGCTCCCAGCATAGATG
  GGAAGGAAGAATTAGATCTGGCTGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGCTGGATTT
  TGAGACTGTTGGAGACATCATTGCCATCATTGAGGACAAGGTAGATGATCATCCTGAAGTGCTGGATGTG
  GCAGCAGTGGAAGCAGCACTGTCATTTTGTGAAGAAAATGATGATCCTCAGTCCCTGCCTGGCCCCTGGG
  AGCATCCTATCCAGCAGGAGCGGGACAAGCCAGTACCTCTCCCTGCACCAGAAATGACGGTCAAGCAAGA
  GAGACTGGACTTTGAGGAAACGGAAAACAAGGGAATACATGAACTGGTGGACATCAGGGAGCCCAGTGCA
  GAGATCAAGGTGGAACCTGCAGAACCAGAGCCAGTCATTTCAGGAGCCGAAATAGTAGCTGGAGTTGTTC
  CAGCCACAAGTATGGAGCCACCAGAACTCAGGAGTCAGGACTTAGATGAGGAACTGGGAAGTACTGCAGC
  TGGAGAGATTGTTGAAGCAGATGTTGCCATTGGGAAAGGCGATGAGACTCCACTTACAAATGTGAAGACA
  GAGGCATCCCCTGAAAGCATGTTGTCTCCATCACATGGCTCAAATCCCATTGAAGATCCTTTAGAGGCAG
  AGACTCAGCACAAGTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGAT
  AAAGGATGCCCCAGGTGAGGATGAGGAGGAAGATGGTGTCAGTGAAGCGGCCAGCCTAGAGGAGCCTAAG
  GAAGAGGATCAAGGAGAAGGCTACTTGTCAGAAATGGATAATGAACCTCCTGTGAGCGAGAGTGATGATG
  GCTTCAGCATACACAATGCTACACTGCAGTCACACACACTGGCAGACTCCATCCCCAGCAGCCCTGCTTC
  TTCACAGTTCTCTGTCTGTAGTGAGGATCAGGAAGCTATTCAGGCACAGAAAATTTGGAAGAAAGCCATC
  ATGCTTGTATGGAGAGCTGCAGCTAATCATAGGTATGCCAATGTCTTCCTGCAGCCTGTTACAGATGACA
  TAGCACCTGGCTACCACAGCATTGTGCAGAGGCCTATGGATTTGTCAACTATTAAGAAAAACATAGAAAA
  TGGACTGATCCGAAGCACAGCTGAATTTCAGCGTGACATTATGCTGATGTTTCAGAATGCTGTAATGTAC
  AATAGCTCAGACCATGATGTCTATCACATGGCAGTGGAGATGCAGCGAGATGTCTTGGAACAGATCCAGC
  AATTCTTGGCCACGCAGTTGATTATGCAAACATCCGAGTCTGGGATCAGTGCTAAAAGTCTTCGAGGGAG
  AGATTCTACCCGCAAACAGGATGCTTCAGAGAAGGACAGTGTCCCAATGGGCTCTCCTGCCTTCCTTCTC
  TCTCTCTTTGATGGAGGAACCAGGGGACGCCGCTGTGCCATTGAAGCAGATATGAAGATGAAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239701 representing NM_001300961
  Red=Cloning site Green=Tags(s)
  
  MVHPREAMFSIFCHEKWRSKLVRVSVSRAIKPFAEPGRPPDWFSQKHCASQYSELLETTETPKRKRGEKG
  EVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDELCNDIATKKKLEEEEA
  EVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLTPTTMEEATSGVNESEMAVASGH
  LNSTGVLLEVGGVLPMIHGGEIQQTPNTVAASPAASGAPTLSRLLEAGPTQFTTPLASFTTVASEPPVKL
  VPPPVESVSQATIVMMPALPAPSSAPAVSTTESVAPVSQPDNCVPMEAVGDPHTVTVSMDSSEISMIINS
  IKEECFRSGVAEAPVGSKAPSIDGKEELDLAEKMDIAVSYTGEELDFETVGDIIAIIEDKVDDHPEVLDV
  AAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEETENKGIHELVDIREPSA
  EIKVEPAEPEPVISGAEIVAGVVPATSMEPPELRSQDLDEELGSTAAGEIVEADVAIGKGDETPLTNVKT
  EASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGEDEEEDGVSEAASLEEPK
  EEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCSEDQEAIQAQKIWKKAI
  MLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTAEFQRDIMLMFQNAVMY
  NSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQDASEKDSVPMGSPAFLL
  SLFDGGTRGRRCAIEADMKMKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300961
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001300961.1, NP_001287890.1
• RefSeq Size: 3039
• RefSeq ORF: 2589
• Locus ID: 10902
• Protein Families: Druggable Genome, Transcription Factors
• MW: 94.4 kDa
• Gene Summary: The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014]