NFATC4 (NM_001288802) Human Tagged ORF Clone

CAT#: RC239742

NFATC4 (myc-DDK-tagged) - Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (NFATC4), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NFATC4
• Synonyms: NF-AT3; NF-ATC4; NFAT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239742 representing NM_001288802
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCTTCAATCTCCTCCATCTTCCCAGGTCCAACTCTGCTTTTGTCTTGTGGCTCAGAAGAACTGG
  ACTCAGAGGATGCCCCGCCATGCTGCCGTCTGGCCTTGGGAGAGCCCCCTCCCTATGGCGCTGCACCTAT
  CGGTATTCCCCGACCTCCACCCCCTCGGCCTGGCATGCATTCGCCACCGCCGCGACCAGCCCCCTCACCT
  GGCACCTGGGAGAGCCAGCCCGCCAGGTCGGTGAGGCTGGGAGGACCAGGAGGGGGTGCTGGGGGTGCTG
  GGGGTGGCCGTGTTCTCGAGTGTCCCAGCATCCGCATCACCTCCATCTCTCCCACGCCGGAGCCGCCAGC
  AGCGCTGGAGGACAACCCTGATGCCTGGGGGGACGGCTCTCCTAGAGATTACCCCCCACCAGAAGGCTTT
  GGGGGCTACAGAGAAGCAGGGGGCCAGGGTGGGGGGGCCTTCTTCAGCCCAAGCCCTGGCAGCAGCAGCC
  TGTCCTCGTGGAGCTTCTTCTCCGATGCCTCTGACGAGGCAGCCCTGTATGCAGCCTGCGACGAGGTGGA
  GTCTGAGCTAAATGAGGCGGCCTCCCGCTTTGGCCTGGGCTCCCCGCTGCCCTCGCCCCGGGCCTCCCCT
  CGGCCATGGACCCCCGAAGATCCCTGGAGCCTGTATGGTCCAAGCCCCGGAGGCCGAGGGCCAGAGGATA
  GCTGGCTACTCCTCAGTGCTCCTGGGCCCACCCCAGCCTCCCCGCGGCCTGCCTCTCCATGTGGCAAGCG
  GCGCTATTCCAGCTCGGGAACCCCATCTTCAGCCTCCCCAGCTCTGTCCCGCCGTGGCAGCCTGGGGGAA
  GAGGGGTCTGAGCCACCTCCACCACCCCCATTGCCTCTGGCCCGGGACCCGGGCTCCCCTGGTCCCTTTG
  ACTATGTGGGGGCCCCACCAGCTGAGAGCATCCCTCAGAAGACACGGCGGACTTCCAGCGAGCAGGCAGT
  GGCTCTGCCTCGGTCTGAGGAGCCTGCCTCATGCAATGGGAAGCTGCCCTTGGGAGCAGAGGAGTCTGTG
  GCTCCTCCAGGAGGTTCCCGGAAGGAGGTGGCTGGCATGGACTACCTGGCAGTGCCCTCCCCACTCGCTT
  GGTCCAAGGCCCGGATTGGGGGACACAGCCCTATCTTCAGGACCTCTGCCCTACCCCCACTGGACTGGCC
  TCTGCCCAGCCAATATGAGCAGCTGGAGCTGAGGATCGAGGTACAGCCTAGAGCCCACCACCGGGCCCAC
  TATGAGACAGAAGGCAGCCGTGGAGCTGTCAAAGCTGCCCCTGGCGGTCACCCCGTAGTCAAGCTCCTAG
  GCTACAGTGAGAAGCCACTGACCCTACAGATGTTCATCGGCACTGCAGATGAAAGGAACCTGCGGCCTCA
  TGCCTTCTATCAGGTGCACCGTATCACAGGCAAGATGGTGGCCACGGCCAGCTATGAAGCCGTAGTCAGT
  GGCACCAAGGTGTTGGAGATGACTCTGCTGCCTGAGAACAACATGGCGGCCAACATTGACTGCGCGGGAA
  TCCTGAAGCTTCGGAATTCAGACATTGAGCTTCGGAAGGGTGAGACGGACATCGGGCGCAAAAACACACG
  TGTACGGCTGGTGTTCCGGGTACACGTGCCCCAGGGCGGCGGGAAGGTCGTCTCAGTACAGGCAGCATCG
  GTGCCCATCGAGTGCTCCCAGCGCTCAGCCCAGGAGCTGCCCCAGGTGGAGGCCTACAGCCCCAGTGCCT
  GCTCTGTGAGAGGAGGCGAGGAACTGGTACTGACTGGCTCCAACTTCCTGCCAGACTCCAAGGTGGTGTT
  CATTGAGAGGGGTCCTGATGGGAAGCTGCAATGGGAGGAGGAGGCCACAGTGAACCGACTGCAGAGCAAC
  GAGGTGACGCTGACCCTGACTGTCCCCGAGTACAGCAACAAGAGGGTTTCCCGGCCAGTCCAGGTCTACT
  TTTATGTCTCCAATGGGCGGAGGAAACGCAGTCCTACCCAGAGTTTCAGGTTTCTGCCTGTGATCTGCAA
  AGAGGAGCCCCTACCGGACTCATCTCTGCGGGGTTTCCCTTCAGCATCGGCAACCCCCTTTGGCACTGAC
  ATGGACTTCTCACCACCCAGGCCCCCCTACCCCTCCTATCCCCATGAAGACCCTGCTTGCGAAACTCCTT
  ACCTATCAGAAGGCTTCGGCTATGGCATGCCCCCTCTGTACCCCCAGACGGGGCCCCCACCATCCTACAG
  ACCGGGCCTGCGGATGTTCCCTGAGACTAGGGGTACCACAGGTTGTGCCCAACCACCTGCAGTTTCCTTC
  CTTCCCCGCCCCTTCCCTAGTGACCCGTATGGAGGGCGGGGCTCCTCTTTCTCCCTGGGGCTGCCATTCT
  CTCCGCCAGCCCCCTTTCGGCCGCCTCCTCTTCCTGCATCCCCACCGCTTGAAGGCCCCTTCCCTTCCCA
  GAGTGATGTGCATCCCCTACCTGCTGAGGGATACAATAAGGTAGGGCCAGGCTATGGCCCTGGGGAGGGG
  GCTCCGGAGCAGGAGAAATCCAGGGGTGGCTACAGCAGCGGCTTCCGAGACAGTGTCCCTATCCAGGGTA
  TCACGCTGGAGGAAGGTGGGTGTGGGACTGGGGGCTGTGAGTGTGAGTGTGTGCAAGAGATTGCTCTGCA
  TGTTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239742 representing NM_001288802
  Red=Cloning site Green=Tags(s)
  
  MPASISSIFPGPTLLLSCGSEELDSEDAPPCCRLALGEPPPYGAAPIGIPRPPPPRPGMHSPPPRPAPSP
  GTWESQPARSVRLGGPGGGAGGAGGGRVLECPSIRITSISPTPEPPAALEDNPDAWGDGSPRDYPPPEGF
  GGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFGLGSPLPSPRASP
  RPWTPEDPWSLYGPSPGGRGPEDSWLLLSAPGPTPASPRPASPCGKRRYSSSGTPSSASPALSRRGSLGE
  EGSEPPPPPPLPLARDPGSPGPFDYVGAPPAESIPQKTRRTSSEQAVALPRSEEPASCNGKLPLGAEESV
  APPGGSRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELRIEVQPRAHHRAH
  YETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERNLRPHAFYQVHRITGKMVATASYEAVVS
  GTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQGGGKVVSVQAAS
  VPIECSQRSAQELPQVEAYSPSACSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQWEEEATVNRLQSN
  EVTLTLTVPEYSNKRVSRPVQVYFYVSNGRRKRSPTQSFRFLPVICKEEPLPDSSLRGFPSASATPFGTD
  MDFSPPRPPYPSYPHEDPACETPYLSEGFGYGMPPLYPQTGPPPSYRPGLRMFPETRGTTGCAQPPAVSF
  LPRPFPSDPYGGRGSSFSLGLPFSPPAPFRPPPLPASPPLEGPFPSQSDVHPLPAEGYNKVGPGYGPGEG
  APEQEKSRGGYSSGFRDSVPIQGITLEEGGCGTGGCECECVQEIALHVC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288802
• ORF Size: 2667 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288802.1, NP_001275731.1
• RefSeq Size: 5538 bp
• RefSeq ORF: 2670 bp
• Locus ID: 4776
• Cytogenetics: 14q12
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• MW: 94.4 kDa
• Gene Summary: 'This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]'