Angiomotin like 1 (AMOTL1) (NM_001301007) Human Tagged ORF Clone

CAT#: RC239759

AMOTL1 (myc-DDK-tagged) - Human angiomotin like 1 (AMOTL1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AMOTL1
• Synonyms: JEAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239759 representing NM_001301007
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGGGCAAAGTTGCGCCGGGGAACTTGTGAGCCTGCGGTGAAAGTTGAAGATCCTCTTTGTAACT
  TCCACTCCCCAAACTTCCTGAGGATCTCAGAGGTGGAAATGAGAGGTTCCGAGGATGCGGCAGCTGGAAC
  AGTATTGCAGCGGCTGATCCAGGAACAACTGCGGTATGGCACCCCAACCGAGAACATGAACTTGCTGGCC
  ATTCAGCACCAGGCCACAGGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCCACGGAAAACC
  TCACTCAAGAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCGCAGGGTCAAGAACACCAGGT
  GGACAATACGGTGATGGAGAAACAGGTCCGGTCCACGCAGCCTCAGCAGAACAACGAGGAACTGCCCACT
  TACGAGGAGGCCAAAGCACAGTCGCAGTTCTTCAGGGGGCAGCAGCAGCAGCAACAGCAGCAGGGGGCGG
  TGGGCCATGGTTACTACATGGCAGGGGGCACCAGTCAGAAGTCCCGAACTGAGGGGAGGCCCACTGTGAA
  CCGTGCCAACAGTGGACAGGCGCATAAGGACGAGGCGCTGAAGGAACTGAAGCAGGGCCACGTCCGCTCG
  CTCAGCGAGAGAATCATGCAGCTGTCCCTGGAGAGGAATGGGGCCAAGCAACACCTTCCCGGCTCGGGGA
  ATGGAAAGGGCTTCAAAGTAGGAGGGGGGCCCTCCCCTGCCCAGCCTGCAGGTAAAGTGCTGGACCCTCG
  GGGTCCTCCACCTGAGTACCCCTTCAAGACCAAGCAAATGATGTCCCCAGTCAGCAAGACCCAGGAGCAC
  GGACTTTTTTATGGTGACCAGCACCCCGGGATGCTCCACGAGATGGTCAAGCCCTACCCTGCTCCTCAGC
  CTGTGAGAACAGATGTGGCCGTCCTGCGGTACCAGCCACCCCCTGAGTATGGGGTAACGAGCCGCCCATG
  CCAACTTCCGTTCCCATCAACCATGCAGCAGCACAGCCCCATGTCCTCCCAGACCTCTTCCGCCAGCGGG
  CCACTGCACTCTGTCTCCCTGCCGCTTCCACTCCCGATGGCCCTGGGTGCTCCACAGCCCCCGCCTGCCG
  CCTCCCCCAGCCAGCAGCTTGGTCCAGATGCCTTTGCGATTGTGGAGCGAGCCCAGCAAATGGTGGAGAT
  ATTAACAGAGGAGAACCGGGTGCTTCACCAGGAACTTCAGGGTTACTACGACAATGCCGACAAGCTCCAC
  AAGTTTGAAAAAGAACTTCAGAGAATTTCGGAAGCCTATGAAAGTCTGGTCAAGTCTACCACCAAGCGAG
  AATCGCTGGACAAGGCCATGAGAAACAAATTGGAAGGCGAGATTAGAAGACTTCATGATTTCAACAGAGA
  CCTCCGAGATCGACTAGAGACTGCTAACAGGCAACTATCCAGCAGGGAATACGAAGGGCATGAAGACAAA
  GCTGCAGAGGGGCATTATGCTTCCCAGAACAAAGAATTCTTGAAGGAAAAGGAGAAATTAGAAATGGAGT
  TAGCAGCAGTGCGGACTGCAAGTGAGGACCATCGGAGACACATCGAGATCCTGGACCAGGCTTTGAGCAA
  CGCCCAGGCCAGGGTCATCAAGCTGGAAGAGGAGTTACGAGAGAAGCAAGCATATGTTGAGAAAGTTGAG
  AAGCTGCAGCAGGCCCTGACCCAGCTGCAGTCTGCATGTGAGAAGCGAGAACAGATGGAGCGGAGACTGC
  GGACTTGGCTGGAGAGAGAGCTGGATGCACTGAGAACCCAGCAGAAACATGGAAATGGCCAGCCAGCCAA
  CATGCCGGAATACAATGCCCCAGCCCTCCTGGAACTTGTGCGGGAGAAGGAGGAGCGGATCCTGGCCCTG
  GAGGCCGACATGACAAAGTGGGAGCAGAAGTACCTGGAGGAGAGCACCATCCGACACTTTGCCATGAATG
  CCGCAGCCACTGCAGCAGCTGAGAGGGACACCACGATCATCAACCACTCACGGAATGGCAGCTACGGAGA
  GAGCTCGCTGGAGGCCCACATCTGGCAAGAGGAGGAGGAGGTGGTGCAGGCCAACAGAAGGTGTCAGGAC
  ATGGAATACACTATTAAAAATCTCCATGCCAAAATCATAGAGAAAGATGCTATGATTAAGGTCCTGCAGC
  AGCGATCTCGTAAAGATGCCGGGAAGACAGACTCCTCCAGCCTACGTCCTGCCCGCTCCGTTCCATCCAT
  AGCAGCAGCTACTGGGACACACTCTCGCCAGACCTCTCTTACCAGCAGCCAGCTGGCTGAGGAAAAGAAG
  GAAGAGAAGACCTGGAAGGGGAGCATAGGATTGCTGCTGGGGAAGGAGCACCATGAGCATGCCTCTGCCC
  CACTGCTGCCACCCCCACCCACCTCAGCACTGTCCTCCATAGCCTCCACTACGGCAGCCAGCAGTGCCCA
  CGCCAAGACAGGCAGCAAGGACAGCAGCACACAGACTGACAAGAGTGCCGAGCTCTTCTGGCCCAGCATG
  GCCTCCCTTCCCAGCCGCGGCCGGCTGAGCACGACCCCTGCTCACAGCCCCGTCCTGAAACACCCAGCGG
  CCAAAGGGACCGCAGAGAAACTGGAGAACTCTCCTGGCCATGGGAAGTCGCCTGACCACAGAGGCCGGGT
  CAGCAGCTTGCTGCACAAGCCCGAGTTCCCTGATGGAGAGATGATGGAAGTCCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239759 representing NM_001301007
  Red=Cloning site Green=Tags(s)
  
  MWRAKLRRGTCEPAVKVEDPLCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLLA
  IQHQATGSAGPAHPTNNFSSTENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNEELPT
  YEEAKAQSQFFRGQQQQQQQQGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGHVRS
  LSERIMQLSLERNGAKQHLPGSGNGKGFKVGGGPSPAQPAGKVLDPRGPPPEYPFKTKQMMSPVSKTQEH
  GLFYGDQHPGMLHEMVKPYPAPQPVRTDVAVLRYQPPPEYGVTSRPCQLPFPSTMQQHSPMSSQTSSASG
  PLHSVSLPLPLPMALGAPQPPPAASPSQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYDNADKLH
  KFEKELQRISEAYESLVKSTTKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSREYEGHEDK
  AAEGHYASQNKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYVEKVE
  KLQQALTQLQSACEKREQMERRLRTWLERELDALRTQQKHGNGQPANMPEYNAPALLELVREKEERILAL
  EADMTKWEQKYLEESTIRHFAMNAAATAAAERDTTIINHSRNGSYGESSLEAHIWQEEEEVVQANRRCQD
  MEYTIKNLHAKIIEKDAMIKVLQQRSRKDAGKTDSSSLRPARSVPSIAAATGTHSRQTSLTSSQLAEEKK
  EEKTWKGSIGLLLGKEHHEHASAPLLPPPPTSALSSIASTTAASSAHAKTGSKDSSTQTDKSAELFWPSM
  ASLPSRGRLSTTPAHSPVLKHPAAKGTAEKLENSPGHGKSPDHRGRVSSLLHKPEFPDGEMMEVLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301007
• ORF Size: 2718 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301007.1, NP_001287936.1
• RefSeq Size: 8849
• RefSeq ORF: 2721
• Locus ID: 154810
• Protein Pathways: Tight junction
• MW: 101.6 kDa
• Gene Summary: The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]