VPS11 (NM_001290185) Human Tagged ORF Clone

CAT#: RC239795

VPS11 (myc-DDK-tagged) - Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS11
• Synonyms: END1; HLD12; hVPS11; PEP5; RNF108
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239795 representing NM_001290185
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCTGTTTGTCGGAGGGGTCCGTGCCGCGCGCCTCTTTGGTTTAGCTGGTCATCCAGAGTTGTTC
  TGTGGTCTACGGGAAGAAAGAAGGAAGTCCATCTCCTAACTTGTTATCAACTGAGCAATCCTGGGAGGCT
  CCTTGACTACCCTGCACATATGGAAGGCCAGATCTGGTTCTTGCCACGTTCCCTACAGCTTACAGGCTTC
  CAAGCCTACAAACTACGGGTGACACACCTGTACCAACTGAAGCAGCACAATATTCTGGCATCTGTTGGAG
  AAGATGAAGAGGGCATCAACCCCTTGGTTAAGATCTGGAACCTGGAGAAGAGAGATGGTGGCAATCCACT
  CTGCACTCGAATCTTCCCTGCTATTCCAGGAACAGAGCCAACTGTTGTATCTTGTTTGACTGTCCATGAA
  AATCTCAACTTTATGGCCATTGGTTTCACAGATGGCAGTGTTACATTGAACAAAGGAGACATCACCCGGG
  ACCGGCATAGCAAGACCCAGATTTTGCACAAGGGCAACTATCCTGTAACTGGATTGGCCTTTCGCCAAGC
  AGGAAAGACCACTCACTTGTTTGTTGTGACAACAGAGAACGTCCAGTCCTATATAGTTTCTGGAAAAGAC
  TACCCTCGCGTGGAGTTGGACACCCATGGTTGTGGCCTGCGCTGCTCAGCCCTAAGTGACCCTTCTCAGG
  ACCTGCAGTTCATTGTGGCCGGGGATGAGTGTGTCTACTTGTACCAGCCTGATGAACGTGGGCCCTGCTT
  CGCCTTTGAGGGCCATAAGCTCATTGCCCACTGGTTTAGAGGCTACCTTATCATTGTCTCCCGTGACCGG
  AAGGTTTCTCCCAAGTCAGAGTTTACCAGCAGGGATTCACAGAGCTCCGACAAGCAGATTCTAAACATCT
  ATGACCTGTGCAACAAGTTCATAGCCTATAGCACCGTCTTTGAGGATGTAGTGGATGTGCTTGCTGAGTG
  GGGCTCCCTGTACGTGCTGACGCGGGATGGGCGGGTCCACGCACTGCAGGAGAAGGACACACAGACCAAA
  CTGGAGATGCTGTTTAAGAAGAACCTATTTGAGATGGCGATTAACCTTGCCAAGAGCCAGCATCTGGACA
  GTGATGGGCTGGCCCAGATTTTCATGCAGTATGGAGACCATCTCTACAGCAAGGGCAACCACGATGGGGC
  TGTCCAGCAATATATCCGAACCATTGGAAAGTTGGAGCCATCCTACGTGATCCGCAAGTTTCTGGATGCC
  CAGCGCATTCACAACCTGACTGCCTACCTGCAGACCCTGCACCGACAATCCCTGGCCAATGCCGACCATA
  CCACCCTGCTCCTCAACTGCTATACCAAGCTCAAGGACAGCTCGAAGCTGGAGGAGTTCATCAAGAAAAA
  GAGTGAGAGTGAAGTCCACTTTGATGTGGAGACAGCCATCAAGGTCCTCCGGCAGGCTGGCTACTACTCC
  CATGCCCTGTATCTGGCGGAGAACCATGCACATCATGAGTGGTACCTGAAGATCCAGCTAGAAGACATTA
  AGAATTATCAGGAAGCCCTTCGATACATCGGCAAGCTGCCTTTTGAGCAGGCAGAGAGCAACATGAAGCG
  CTACGGCAAGATCCTCATGCACCACATACCAGAGCAGACAACTCAGTTGCTGAAGGGACTTTGTACTGAT
  TATCGGCCCAGCCTCGAAGGCCGCAGCGATAGGGAGGCCCCAGGCTGCAGGGCCAACTCTGAGGAGTTCA
  TCCCCATCTTTGCCAATAACCCGCGAGAGCTGAAAGCCTTCCTAGAGCACATGAGTGAAGTGCAGCCAGA
  CTCACCCCAGGGGATCTACGACACACTCCTTGAGCTGCGACTGCAGAACTGGGCCCACGAGAAGGATCCA
  CAGGTCAAAGAGAAGCTTCACGCAGAGGCCATTTCCCTGCTGAAGAGTGGTCGCTTCTGCGACGTCTTTG
  ACAAGGCCCTGGTCCTGTGCCAGATGCACGACTTCCAGGATGGTGTCCTTTACCTTTATGAGCAGGGGAA
  GCTGTTCCAGCAGATCATGCACTACCACATGCAGCACGAGCAGTACCGGCAGGTCATCAGCGTGTGTGAG
  CGCCATGGGGAGCAGGACCCCTCCTTGTGGGAGCAGGCCCTCAGCTACTTCGCTCGCAAGGAGGAGGACT
  GCAAGGAGTATGTGGCAGCTGTCCTCAAGCATATCGAGAACAAGAACCTCATGCCACCTCTTCTAGTGGT
  GCAGACCCTGGCCCACAACTCCACAGCCACACTCTCCGTCATCAGGGACTACCTGGTCCAAAAACTACAG
  AAACAGAGCCAGCAGATTGCACAGGATGAGCTGCGGGTGCGGCGGTACCGAGAGGAGACCACCCGTATCC
  GCCAGGAGATCCAAGAGCTCAAGGCCAGTCCTAAGATTTTCCAAAAGACCAAGTGCAGCATCTGTAACAG
  TGCCTTGGAGTTGCCCTCAGTCCACTTCCTGTGTGGCCACTCCTTCCACCAACACTGCTTTGAGAGTTAC
  TCGGAAAGTGATGCTGACTGCCCCACCTGCCTCCCTGAAAACCGGAAGGTCATGGATATGATCCGGGCCC
  AGGAACAGAAACGAGATCTCCATGATCAATTCCAGCATCAGCTCAAGTGCTCCAATGACAGCTTTTCTGT
  GATTGCTGACTACTTTGGCAGAGGTGTTTTCAACAAATTGACTCTGCTGACCGACCCTCCCACAGCCAGA
  CTGACCTCCAGCCTGGAGGCTGGGCTGCAACGCGACCTACTCATGCACTCCAGGAGGGGCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239795 representing NM_001290185
  Red=Cloning site Green=Tags(s)
  
  MKSVCRRGPCRAPLWFSWSSRVVLWSTGRKKEVHLLTCYQLSNPGRLLDYPAHMEGQIWFLPRSLQLTGF
  QAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEPTVVSCLTVHE
  NLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTENVQSYIVSGKD
  YPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFRGYLIIVSRDR
  KVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVHALQEKDTQTK
  LEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEPSYVIRKFLDA
  QRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAIKVLRQAGYYS
  HALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQTTQLLKGLCTD
  YRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDP
  QVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCE
  RHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLSVIRDYLVQKLQ
  KQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGHSFHQHCFESY
  SESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLKCSNDSFSVIADYFGRGVFNKLTLLTDPPTAR
  LTSSLEAGLQRDLLMHSRRGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290185
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001290185.1, NP_001277114.1
• RefSeq Size: 3486
• RefSeq ORF: 2796
• Locus ID: 55823
• Protein Families: Druggable Genome
• MW: 107.8 kDa
• Gene Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]