POLR1B (NM_001282774) Human Tagged ORF Clone

CAT#: RC239815

POLR1B (myc-DDK-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLR1B
• Synonyms: RPA2; RPA135; Rpo1-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239815 representing NM_001282774
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACTGACCCCTCTT
  ATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTTGCAGGAGCTGACGCGGGCGCACGTGGAGTCCTTCAA
  CTACGCTGTGCACGAGGGTCTCGGCCTCGCGGTGCAGGCTATACCTCCCTTTGAATTTGCTTTCAAAGAT
  GAGCGTATCTCTTTTACTATTCTGGATGCTGTTATCAGTCCACCTACAGTTCCAAAAGGGACCATCTGCA
  AAGAGGCCAATGTTTATCCAGCAGAATGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGACAGCTGA
  TATCAACTGGGCAGTGAATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCCATCATG
  GTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGCCCTCATTGAGCACCATGAGGAGGCAG
  AGGAAATGGGGGGCTATTTTATAATCAATGGCATTGAAAAAGTCATCCGAATGTTGATTATGCCTCGGAG
  AAATTTTCCCATTGCAATGATAAGACCAAAATGGAAAACCAGAGGGCCTGGTTATACTCAGTATGGAGTT
  TCAATGCACTGTGTGAGGGAAGAACATTCCGCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTG
  TTATGTTGAACTTTATTTACCGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTTGT
  CAGCTTTTCTGATTATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAAC
  TCTGTTTCTCAGATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACACAAAAACAGGTCCTTAACTACC
  TAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAATGAGCAAGCTGCGGAGTTCCT
  GTTTAACCAGTGCATCTGTATCCACTTGAAATCCAATACTGAAAAGTTTTATATGCTTTGTCTCATGACG
  CGAAAGCTCTTTGCTTTAGCCAAAGGAGAGTGCATGGAGGACAATCCTGATAGTTTGGTGAACCAGGAAG
  TCCTCACACCGGGTCAGCTCTTCCTTATGTTCCTGAAGGGGTCACTCCCATTGATGGAGCTCCCCACCGA
  TCATACAGTGTTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTTATACCCATGACAGGAAAA
  CCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTGGTACGGCCTGTGCAGAACTTAG
  CATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACAGATCTTCATGAATGTCGCTATCTTTGAGGATGA
  AGTTTTTGCTGGAGTTACCACACACCAGGAACTCTTTCCACACAGCCTGCTGAGTGTGATTGCCAACTTC
  ATCCCTTTCTCTGATCACAACCAGAGTCCACGGAACATGTACCAATGCCAGATGGGTAAGCAAACTATGG
  GCTTTCCACTTCTCACTTATCAAGACCGATCGGATAACAAACTGTATCGTCTTCAGACTCCTCAGAGTCC
  CTTGGTGAGACCCTCCATGTATGATTATTATGACATGGATAACTATCCAATTGGGACCAATGCCATCGTT
  GCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATTGTGAATAAGGCCTCTTGGGAACGAG
  GCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGACCTCTCTGAAAAAATTAAACAAGGAGATAG
  TAGCCTGGTGTTTGGCATCAAACCTGGTGACCCACGCGTTCTGCAGAAGTTAGATGACGATGGATTGCCG
  TTTATAGGAGCAAAACTGCAGTACGGAGATCCGTATTACAGCTACCTCAACCTCAACACCGGGGAAAGTT
  TTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGTGGATAACATCAAAGTGTGCAGTAATGACACTGG
  GAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGGAACCCAACTATCGGAGATAAATTT
  GCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTGTGGCCGGCTGAGGACATGCCTTTTACTGAGA
  GTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTTTTCCATCCCGCATGACCATTGGGATGTTAAT
  TGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCA
  GAGGAGAACTCGGCCTTAGAATACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAATTTCTATGGCACCG
  AGAGGTTATATAGTGGCATCAGTGGGCTAGAACTGGAAGCAGACATCTTCATAGGAGTGGTTTATTATCA
  GCGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAGGACAACTGGAGCCCGAGACAGAGTCACCAAC
  CAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGTTTTGGGGAGATGGAACGGGATGCGCTTTTAG
  CTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTCAACTGCTCAGATCGGTCGGTAGCCCATGTGTG
  TGTGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAGAAGCCACCCCCTTCTTGGTCTGCCATGCGCAAC
  AGAAAATACAACTGTACTCTGTGTAGTCGCAGTGACACTATCGATACTGTTTCTGTGCCTTATGTTTTTC
  GGTATTTTGTAGCTGAACTGGCAGCTATGAACATCAAAGTGAAACTGGATGTTGTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239815 representing NM_001282774
  Red=Cloning site Green=Tags(s)
  
  MDPGSRWRNLPSGPSLKHLTDPSYGIPREQQKAALQELTRAHVESFNYAVHEGLGLAVQAIPPFEFAFKD
  ERISFTILDAVISPPTVPKGTICKEANVYPAECRGRRSTYRGKLTADINWAVNGISKGIIKQFLGYVPIM
  VKSKLCNLRNLPPQALIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRGPGYTQYGV
  SMHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDSFLRN
  SVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICIHLKSNTEKFYMLCLMT
  RKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKGSLPLMELPTDHTVLREKRIPPWMEVVLIPMTGK
  PSLYPGLFLFTTPCRLVRPVQNLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANF
  IPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIV
  AVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLP
  FIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKF
  ASRHGQKGILSRLWPAEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFS
  EENSALEYFGEMLKAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTN
  QPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRN
  RKYNCTLCSRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282774
• ORF Size: 2856 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282774.1, NP_001269703.1
• RefSeq Size: 4878
• RefSeq ORF: 2859
• Locus ID: 84172
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 108.3 kDa
• Gene Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]). [supplied by OMIM, Mar 2008]