POLR1B (NM_001282774) Human Untagged Clone

CAT#: SC337709

POLR1B (untagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR1B
• Synonyms: RPA2; RPA135; Rpo1-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001282774, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACTGACCCCTCTT
  ATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTTGCAGGAGCTGACGCGGGCGCACGTGGAGTCCTTCAA
  CTACGCTGTGCACGAGGGTCTCGGCCTCGCGGTGCAGGCTATACCTCCCTTTGAATTTGCTTTCAAAGAT
  GAGCGTATCTCTTTTACTATTCTGGATGCTGTTATCAGTCCACCTACAGTTCCAAAAGGGACCATCTGCA
  AAGAGGCCAATGTTTATCCAGCAGAATGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGACAGCTGA
  TATCAACTGGGCAGTGAATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCCATCATG
  GTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGCCCTCATTGAGCACCATGAGGAGGCAG
  AGGAAATGGGGGGCTATTTTATAATCAATGGCATTGAAAAAGTCATCCGAATGTTGATTATGCCTCGGAG
  AAATTTTCCCATTGCAATGATAAGACCAAAATGGAAAACCAGAGGGCCTGGTTATACTCAGTATGGAGTT
  TCAATGCACTGTGTGAGGGAAGAACATTCCGCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTG
  TTATGTTGAACTTTATTTACCGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTTGT
  CAGCTTTTCTGATTATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAAC
  TCTGTTTCTCAGATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACACAAAAACAGGTCCTTAACTACC
  TAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAATGAGCAAGCTGCGGAGTTCCT
  GTTTAACCAGTGCATCTGTATCCACTTGAAATCCAATACTGAAAAGTTTTATATGCTTTGTCTCATGACG
  CGAAAGCTCTTTGCTTTAGCCAAAGGAGAGTGCATGGAGGACAATCCTGATAGTTTGGTGAACCAGGAAG
  TCCTCACACCGGGTCAGCTCTTCCTTATGTTCCTGAAGGGGTCACTCCCATTGATGGAGCTCCCCACCGA
  TCATACAGTGTTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTTATACCCATGACAGGAAAA
  CCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTGGTACGGCCTGTGCAGAACTTAG
  CATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACAGATCTTCATGAATGTCGCTATCTTTGAGGATGA
  AGTTTTTGCTGGAGTTACCACACACCAGGAACTCTTTCCACACAGCCTGCTGAGTGTGATTGCCAACTTC
  ATCCCTTTCTCTGATCACAACCAGAGTCCACGGAACATGTACCAATGCCAGATGGGTAAGCAAACTATGG
  GCTTTCCACTTCTCACTTATCAAGACCGATCGGATAACAAACTGTATCGTCTTCAGACTCCTCAGAGTCC
  CTTGGTGAGACCCTCCATGTATGATTATTATGACATGGATAACTATCCAATTGGGACCAATGCCATCGTT
  GCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATTGTGAATAAGGCCTCTTGGGAACGAG
  GCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGACCTCTCTGAAAAAATTAAACAAGGAGATAG
  TAGCCTGGTGTTTGGCATCAAACCTGGTGACCCACGCGTTCTGCAGAAGTTAGATGACGATGGATTGCCG
  TTTATAGGAGCAAAACTGCAGTACGGAGATCCGTATTACAGCTACCTCAACCTCAACACCGGGGAAAGTT
  TTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGTGGATAACATCAAAGTGTGCAGTAATGACACTGG
  GAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGGAACCCAACTATCGGAGATAAATTT
  GCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTGTGGCCGGCTGAGGACATGCCTTTTACTGAGA
  GTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTTTTCCATCCCGCATGACCATTGGGATGTTAAT
  TGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCA
  GAGGAGAACTCGGCCTTAGAATACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAATTTCTATGGCACCG
  AGAGGTTATATAGTGGCATCAGTGGGCTAGAACTGGAAGCAGACATCTTCATAGGAGTGGTTTATTATCA
  GCGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAGGACAACTGGAGCCCGAGACAGAGTCACCAAC
  CAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGTTTTGGGGAGATGGAACGGGATGCGCTTTTAG
  CTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTCAACTGCTCAGATCGGTCGGTAGCCCATGTGTG
  TGTGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAGAAGCCACCCCCTTCTTGGTCTGCCATGCGCAAC
  AGAAAATACAACTGTACTCTGTGTAGTCGCAGTGACACTATCGATACTGTTTCTGTGCCTTATGTTTTTC
  GGTATTTTGTAGCTGAACTGGCAGCTATGAACATCAAAGTGAAACTGGATGTTGTTTAA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001282774
• ORF Size: 2859 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_001282774.1, NP_001269703.1
• RefSeq Size: 4878
• RefSeq ORF: 2859
• Locus ID: 84172
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (4) lacks two alternate exons that result in a short frameshift in the central coding region, and also uses an alternate splice site that restores the reading frame in the remaining coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.