RGS3 (NM_001282923) Human Tagged ORF Clone

CAT#: RC239957

RGS3 (myc-DDK-tagged) - Human regulator of G-protein signaling 3 (RGS3), transcript variant 10

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGS3
• Synonyms: C2PA; RGP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239957 representing NM_001282923
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGGACCGGGTTCTGCTGCTTCACAGTCTCTTTATTCGTGTGGATGGATATGTCTATGTGTGTCT
  CTCTTTCTCGCTGTGTGTGTGTGTATGTTTCCATTCATCCACCCCAATGTCTGAATTCTCTTTTAGTTAT
  AGAAGGTAAAGGCCTGATCAGCAAACAGCCTGGCACCTGTGATCCGTATGTGAAGATTTCTTTGATCCCT
  GAAGATAGTAGACTACGCCACCAGAAGACGCAGACCGTTCCAGACTGCAGAGACCCGGCTTTCCACGAGC
  ACTTCTTCTTTCCTGTCCAAGAGGAGGATGATCAGAAGCGTCTCTTGGTTACTGTGTGGAACAGGGCCAG
  CCAGTCCAGACAGAGTGGACTCATTGGCTGCATGAGCTTTGGGGTGAAGTCTCTCCTGACTCCAGACAAG
  GAGATCAGTGGTTGGTACTACCTCCTAGGGGAGCACCTGGGCCGGACCAAGCACTTGAAGGTGGCCAGGC
  GGCGACTGCGGCCGCTGAGAGACCCGCTGCTGAGAATGCCAGGAGGTGGGGACACTGAGAATGGGAAGAA
  ACTAAAGATCACCATCCCGAGGGGAAAGGACGGCTTTGGCTTCACCATCTGCTGCGACTCTCCAGTTCGA
  GTCCAGGCCGTGGATTCCGGGGGTCCGGCGGAACGGGCAGGGCTGCAGCAGCTGGACACGGTGCTGCAGC
  TGAATGAGAGGCCTGTGGAGCACTGGAAATGTGTGGAGCTGGCCCACGAGATCCGGAGCTGCCCCAGTGA
  GATCATCCTACTCGTGTGGCGCATGGTCCCCCAGGTCAAGCCAGGACCAGATGGCGGGGTCCTGCGGCGG
  GCCTCCTGCAAGTCGACACATGACCTCCAGTCACCCCCCAACAAACGGGAGAAGAACTGCACCCATGGGG
  TCCAGGCACGGCCTGAGCAGCGCCACAGCTGCCACCTGGTATGTGACAGCTCTGATGGGCTGCTGCTCGG
  CGGCTGGGAGCGCTACACCGAGGTGGCCAAGCGCGGGGGCCAGCACACCCTGCCTGCACTGTCCCGTGCC
  ACTGCCCCCACCGACCCCAACTACATCATCCTGGCCCCGCTGAATCCTGGGAGCCAGCTGCTCCGGCCTG
  TGTACCAGGAGGATACCATCCCCGAAGAATCAGGGAGTCCCAGTAAAGGGAAGTCCTACACAGGCCTGGG
  GAAGAAGTCCCGGCTGATGAAGACAGTGCAGACCATGAAGGGCCACGGGAACTACCAAAACTGCCCGGTT
  GTGAGGCCGCATGCCACGCACTCAAGCTATGGCACCTACGTCACCCTGGCCCCCAAAGTCCTGGTGTTCC
  CTGTCTTTGTTCAGCCTCTAGATCTCTGTAATCCTGCCCGGACCCTCCTGCTGTCAGAGGAGCTGCTGCT
  GTATGAAGGGAGGAACAAGGCTGCCGAGGTGACACTGTTTGCCTATTCGGACCTGCTGCTCTTCACCAAG
  GAGGACGAGCCTGGCCGCTGCGACGTCCTGAGGAACCCCCTCTACCTCCAGAGTGTGAAGCTGCAGGAAG
  GTTCTTCAGAAGACCTGAAATTCTGCGTGCTCTATCTAGCAGAGAAGGCAGAGTGCTTATTCACTTTGGA
  AGCGCACTCGCAGGAGCAGAAGAAGAGAGTGTGCTGGTGCCTGTCGGAGAACATCGCCAAGCAGCAACAG
  CTGGCAGCATCACCCCCGGACAGCAAGATGTTTGAGACGGAGGCAGATGAGAAGAGGGAGATGGCCTTGG
  AGGAAGGGAAGGGGCCTGGTGCCGAGGATTCCCCACCCAGCAAGGAGCCCTCTCCTGGCCAGGAGCTTCC
  TCCAGGACAAGACCTTCCACCCAACAAGGACTCCCCTTCTGGGCAGGAACCCGCTCCCAGCCAAGAACCA
  CTGTCCAGCAAAGACTCAGCTACCTCTGAAGGATCCCCTCCAGGCCCAGATGCTCCGCCCAGCAAGGATG
  TGCCACCATGCCAGGAACCCCCTCCAGCCCAAGACCTCTCACCCTGCCAGGACCTACCTGCTGGTCAAGA
  ACCCCTGCCTCACCAGGACCCTCTACTCACCAAAGACCTCCCTGCCATCCAGGAATCCCCCACCCGGGAC
  CTTCCACCCTGTCAAGATCTGCCTCCTAGCCAGGTCTCCCTGCCAGCCAAGGCCCTTACTGAGGACACCA
  TGAGCTCCGGGGACCTACTAGCAGCTACTGGGGACCCACCTGCGGCCCCCAGGCCAGCCTTCGTGATCCC
  TGAGGTCCGGCTGGATAGCACCTACAGCCAGAAGGCAGGGGCAGAGCAGGGCTGCTCGGGAGATGAGGAG
  GATGCAGAAGAGGCCGAGGAGGTGGAGGAGGGGGAGGAAGGGGAGGAGGACGAGGATGAGGACACCAGCG
  ATGACAACTACGGAGAGCGCAGTGAGGCCAAGCGCAGCAGCATGATCGAGACGGGCCAGGGGGCTGAGGG
  TGGCCTCTCACTGCGTGTGCAGAACTCGCTGCGGCGCCGGACGCACAGCGAGGGCAGCCTGCTGCAGGAG
  CCCCGAGGGCCCTGCTTTGCCTCCGACACCACCTTGCACTGCTCAGACGGTGAGGGCGCCGCCTCCACCT
  GGGGCATGCCTTCGCCCAGCACCCTCAAGAAAGAGCTGGGCCGCAATGGTGGCTCCATGCACCACCTTTC
  CCTCTTCTTCACAGGACACAGGAAGATGAGCGGGGCTGACACCGTTGGGGATGATGACGAAGCCTCCCGG
  AAGAGAAAGAGCAAAAACCTAGCCAAGGACATGAAGAACAAGCTGGGGATCTTCAGACGGCGGAATGAGT
  CCCCTGGAGCCCCTCCCGCGGGCAAGGCAGACAAAATGATGAAGTCATTCAAGCCCACCTCAGAGGAAGC
  CCTCAAGTGGGGCGAGTCCTTGGAGAAGCTGCTGGTTCACAAATACGGGTTAGCAGTGTTCCAAGCCTTC
  CTTCGCACTGAGTTCAGTGAGGAGAATCTGGAGTTCTGGTTGGCTTGTGAGGACTTCAAGAAGGTCAAGT
  CACAGTCCAAGATGGCATCCAAGGCCAAGAAGATCTTTGCTGAATACATCGCGATCCAGGCATGCAAGGA
  GGTCAACCTGGACTCCTACACGCGGGAGCACACCAAGGACAACCTGCAGAGCGTCACGCGGGGCTGCTTC
  GACCTGGCACAGAAGCGCATCTTCGGGCTCATGGAAAAGGACTCGTACCCTCGCTTTCTCCGTTCTGACC
  TCTACCTGGACCTTATTAACCAGAAGAAGATGAGTCCCCCGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239957 representing NM_001282923
  Red=Cloning site Green=Tags(s)
  
  MPRTGFCCFTVSLFVWMDMSMCVSLSRCVCVYVSIHPPQCLNSLLVIEGKGLISKQPGTCDPYVKISLIP
  EDSRLRHQKTQTVPDCRDPAFHEHFFFPVQEEDDQKRLLVTVWNRASQSRQSGLIGCMSFGVKSLLTPDK
  EISGWYYLLGEHLGRTKHLKVARRRLRPLRDPLLRMPGGGDTENGKKLKITIPRGKDGFGFTICCDSPVR
  VQAVDSGGPAERAGLQQLDTVLQLNERPVEHWKCVELAHEIRSCPSEIILLVWRMVPQVKPGPDGGVLRR
  ASCKSTHDLQSPPNKREKNCTHGVQARPEQRHSCHLVCDSSDGLLLGGWERYTEVAKRGGQHTLPALSRA
  TAPTDPNYIILAPLNPGSQLLRPVYQEDTIPEESGSPSKGKSYTGLGKKSRLMKTVQTMKGHGNYQNCPV
  VRPHATHSSYGTYVTLAPKVLVFPVFVQPLDLCNPARTLLLSEELLLYEGRNKAAEVTLFAYSDLLLFTK
  EDEPGRCDVLRNPLYLQSVKLQEGSSEDLKFCVLYLAEKAECLFTLEAHSQEQKKRVCWCLSENIAKQQQ
  LAASPPDSKMFETEADEKREMALEEGKGPGAEDSPPSKEPSPGQELPPGQDLPPNKDSPSGQEPAPSQEP
  LSSKDSATSEGSPPGPDAPPSKDVPPCQEPPPAQDLSPCQDLPAGQEPLPHQDPLLTKDLPAIQESPTRD
  LPPCQDLPPSQVSLPAKALTEDTMSSGDLLAATGDPPAAPRPAFVIPEVRLDSTYSQKAGAEQGCSGDEE
  DAEEAEEVEEGEEGEEDEDEDTSDDNYGERSEAKRSSMIETGQGAEGGLSLRVQNSLRRRTHSEGSLLQE
  PRGPCFASDTTLHCSDGEGAASTWGMPSPSTLKKELGRNGGSMHHLSLFFTGHRKMSGADTVGDDDEASR
  KRKSKNLAKDMKNKLGIFRRRNESPGAPPAGKADKMMKSFKPTSEEALKWGESLEKLLVHKYGLAVFQAF
  LRTEFSEENLEFWLACEDFKKVKSQSKMASKAKKIFAEYIAIQACKEVNLDSYTREHTKDNLQSVTRGCF
  DLAQKRIFGLMEKDSYPRFLRSDLYLDLINQKKMSPPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282923
• ORF Size: 3264 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282923.1, NP_001269852.1
• RefSeq Size: 4279 bp
• RefSeq ORF: 3267 bp
• Locus ID: 5998
• Cytogenetics: 9q32
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 120.7 kDa
• Gene Summary: 'This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]'