AIP1 (MAGI2) (NM_001301128) Human Tagged ORF Clone

CAT#: RC240148

MAGI2 (myc-DDK-tagged) - Human membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAGI2
• Synonyms: ACVRIP1; AIP-1; AIP1; ARIP1; MAGI-2; NPHS15; SSCAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240148 representing NM_001301128
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAAAGCTTGAAAAAGAAAAGCCACTGGACTAGCAAAGTCCATGAGAGTGTCATTGGCAGGAACC
  CGGAGGGCCAGCTGGGCTTTGAACTGAAGGGGGGCGCCGAGAATGGACAGTTCCCCTACCTGGGGGAGGT
  GAAGCCCGGCAAGGTGGCCTATGAGAGCGGCAGCAAATTGGTGTCGGAGGAGCTGCTGCTGGAGGTGAAC
  GAGACCCCCGTGGCGGGGCTCACCATCAGGGACGTGCTGGCCGTGATCAAACACTGCAAGGACCCCCTCC
  GGCTCAAGTGTGTCAAGCAAGGAGGAATTGTTGATAAAGACCTTCGTCACTACCTCAACTTACGATTTCA
  AAAGGGTTCTGTGGACCATGAGCTTCAGCAAATCATTCGTGACAACCTCTACCTCCGCACGGTGCCATGC
  ACCACAAGGCCACATAAGGAGGGTGAGGTCCCTGGAGTGGATTATATTTTCATCACTGTTGAAGATTTTA
  TGGAATTGGAGAAAAGTGGTGCTCTCCTAGAAAGTGGGACTTATGAAGACAATTACTACGGTACCCCAAA
  GCCGCCAGCAGAACCAGCACCATTATTGTTAAATGTAACAGACCAGATACTTCCAGGAGCCACTCCAAGT
  GCTGAAGGAAAACGGAAGAGGAATAAATCAGTGAGCAACATGGAGAAAGCCAGTATAGAGCCTCCTGAGG
  AGGAAGAGGAAGAGAGGCCTGTGGTCAATGGAAATGGAGTAGTAGTAACACCAGAATCCAGTGAACATGA
  AGACAAAAGTGCAGGTGCCTCAGGGGAGATGCCCTCCCAGCCTTATCCTGCACCAGTGTACAGTCAGCCT
  GAGGAGCTGAAGGAGCAGATGGATGACACAAAGCCAACTAAACCTGAAGACAATGAGGAACCAGACCCAT
  TGCCTGATAACTGGGAAATGGCCTATACAGAGAAGGGCGAAGTCTACTTCATTGACCATAACACAAAGAC
  AACATCATGGCTGGATCCACGACTTGCGAAAAAGGCTAAACCTCCAGAAGAGTGCAAAGAAAATGAGCTT
  CCATATGGCTGGGAAAAAATCGATGATCCCATTTATGGCACTTATTATGTTGACCACATAAATAGAAGAA
  CACAGTTTGAAAATCCTGTCCTGGAAGCAAAAAGGAAGCTACAGCAACATAACATGCCCCACACAGAACT
  TGGAACAAAGCCCCTGCAGGCCCCAGGTTTCCGAGAAAAACCACTCTTCACCCGGGATGCATCCCAGTTG
  AAGGGAACATTCCTCAGCACCACCCTAAAAAAGAGCAACATGGGCTTTGGATTTACCATCATTGGTGGAG
  ACGAGCCTGATGAGTTTCTGCAGGTGAAAAGTGTGATTCCGGATGGGCCTGCAGCACAGGATGGAAAAAT
  GGAAACAGGTGATGTCATTGTCTATATTAATGAAGTTTGTGTCCTTGGACACACTCATGCAGATGTTGTC
  AAACTTTTCCAGTCTGTTCCTATTGGTCAGAGTGTCAACCTGGTGTTGTGTCGTGGCTACCCTTTGCCCT
  TTGATCCTGAAGACCCTGCTAACAGCATGGTGCCACCCCTTGCAATAATGGAGAGGCCACCTCCAGTGAT
  GGTCAATGGAAGACACAACTATGAAACATATTTGGAGTACATTTCTCGGACCTCACAGTCAGTTCCAGAT
  ATAACAGATCGGCCGCCTCATTCTCTGCACTCCATGCCAACTGATGGTCAGCTAGACGGCACGTATCCAC
  CGCCCGTCCATGATGACAATGTGTCTATGGCTTCATCTGGGGCCACCCAAGCTGAACTTATGACCTTAAC
  CATTGTGAAAGGTGCCCAGGGCTTCGGCTTCACTATTGCCGACAGTCCTACAGGACAGCGGGTGAAACAA
  ATACTTGACATTCAGGGATGCCCTGGCCTGTGTGAAGGCGACCTCATTGTTGAGATCAACCAGCAGAATG
  TACAGAACCTGAGCCATACAGAAGTAGTGGATATACTTAAGGACTGTCCCATTGGAAGTGAAACTTCTTT
  GATTATCCATCGAGGAGGTTTCTTTTCTCCATGGAAAACTCCAAAGCCTATAATGGACCGATGGGAGAAT
  CAAGGCAGTCCTCAAACGAGTTTATCTGCTCCGGCCATACCGCAGAACCTGCCCTTCCCACCTGCCCTTC
  ACAGGAGCTCCTTTCCTGACTCAACAGAGGCCTTTGACCCACGGAAGCCTGATCCATATGAGCTCTACGA
  GAAATCTAGGGCCATTTATGAAAGTAGGCGTCCAGATTATAAGGAATTGGATGTTCATCTTCGGAGGATG
  GAGTCTGGATTTGGCTTCAGAATCCTCGGGGGAGATGAGCCTGGACAGCCTATTTTGATTGGAGCTGTCA
  TTGCCATGGGCTCAGCCGACAGAGATGGCCGCCTTCACCCAGGAGATGAGCTTGTGTATGTTGATGGGAT
  TCCAGTAGCCGGCAAAACCCACCGCTATGTCATCGACCTCATGCACCACGCAGCCCGCAATGGGCAGGTC
  AACCTCACTGTGAGAAGAAAGGTGCTATGTGGAGGGGAGCCCTGCCCAGAGAACGGGAGAAGTCCAGGCT
  CTGTATCCACCCACCACAGCTCTCCACGCAGTGACTACGCAACCTACACCAACAGCAACCACGCTGCCCC
  CAGTAGCAATGCCTCTCCCCCTGAAGGCTTCGCCTCCCACAGCCTGCAGACCAGTGATGTGGTCATTCAC
  CGCAAAGAGAATGAGGGCTTCGGCTTTGTCATCATCAGCTCCCTGAACAGGCCTGAGTCTGGATCCACTA
  TAACTGTGCCCCATAAAATCGGACGCATCATTGATGGGAGTCCTGCAGATCGCTGTGCAAAACTAAAAGT
  GGGAGACCGGATCCTAGCAGTGAATGGCCAGTCTATCATCAACATGCCTCACGCTGACATCGTGAAGCTC
  ATCAAGGATGCAGGTCTTAGTGTCACCCTTCGCATCATTCCTCAGGAGGAGCTCAACAGCCCCACCTCGG
  CACCCAGCTCAGAGAAGCAGAGTCCCATGGCGCAGCAGAGTCCCCTGGCACAGCAGAGTCCCCTGGCCCA
  GCCAAGCCCAGCCACCCCCAACAGCCCCATCGCCCAGCCAGCACCACCTCAACCACTTCAGCTGCAAGGA
  CACGAAAATAGTTACAGGTCGGAAGTGAAAGCAAGGCAAGATGTGAAACCAGACATCCGACAGCCTCCAT
  TCACAGACTACAGGCAGCCCCCGCTGGATTACAGGCAACCCCCAGGAGGGGACTACCAGCAGCCCCCACC
  CTTGGACTACAGGCAGCCTCCCCTACTGGACTACAGGCAGCACTCCCCCGACACCAGGCAGTACCCTCTG
  TCGGACTACAGACAACCCCAGGATTTTGATTATTTCACTGTGGACATGGAGAAAGGAGCCAAAGGATTTG
  GATTCAGCATTCGTGGAGGAAGGGAATACAAAATGGATTTGTATGTGTTGAGACTGGCAGAAGATGGACC
  AGCAATAAGGAATGGGAGGATGAGGGTAGGAGATCAAATCATTGAAATCAATGGGGAAAGCACAAGGGAC
  ATGACACATGCCAGAGCAATAGAACTCATCAAATCTGGAGGAAGACGAGTGAGGCTGCTGCTCAAGAGAG
  GCACGGGACAGGTCCCAGAATATGACGAACCCGCCCCCTGGAGTTCTCCCGCTGCCGCCGCCCCAGGTCT
  GCCGGAAGTAGGCGTCTCCCTGGACGACGGCCTCGCTCCATTCTCTCCATCACATCCAGCCCCACCCTCC
  GACCCTTCCCACCAGATAAGCCCAGGCCCAACTTGGGATATCAAACGGGAACACGACGTTAGGAAACCAA
  AGGAGCTTTCAGCCTGCGGCCAGAAGAAGCAGCGCCTCGGGGAGCAGAGGGAGCGCTCGGCGAGTCCGCA
  GAGGGCCGCGCGGCCGAGGCTCGAGGAGGCGCCCGGCGGCCAGGGGCGGCCCGAGGCCGGCAGGCCCGCC
  TCGGAGGCCAGGGCGCCCGGGCTCGCGGCGGCAGACGCGGCGGACGCGGCGCGGGCGGGCGGGAAGGAGG
  CGCCCCGTGCGGCGGCGGGCTCCGAGCTCTGCCGGCGCGAAGGCCCGGGGGCTGCGCCGGCGTTTGCCGG
  CCCGGGCGGCGGCGGCAGCGGCGCGCTGGAGGCCGAGGGCAGGGCGGGTGCGCGCGCGGGTCCCCGGCCG
  GGCCCGCGACCCCCGGGGGGCGCCCCGGCGCGCAAGGCCGCCGTCGCGCCGGGGCCCTGGAAGGTGCCGG
  GTTCTGACAAGCTGCCCAGCGTCCTCAAACCCGGCGCCTCGGCCGCCAGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240148 representing NM_001301128
  Red=Cloning site Green=Tags(s)
  
  MSKSLKKKSHWTSKVHESVIGRNPEGQLGFELKGGAENGQFPYLGEVKPGKVAYESGSKLVSEELLLEVN
  ETPVAGLTIRDVLAVIKHCKDPLRLKCVKQGGIVDKDLRHYLNLRFQKGSVDHELQQIIRDNLYLRTVPC
  TTRPHKEGEVPGVDYIFITVEDFMELEKSGALLESGTYEDNYYGTPKPPAEPAPLLLNVTDQILPGATPS
  AEGKRKRNKSVSNMEKASIEPPEEEEEERPVVNGNGVVVTPESSEHEDKSAGASGEMPSQPYPAPVYSQP
  EELKEQMDDTKPTKPEDNEEPDPLPDNWEMAYTEKGEVYFIDHNTKTTSWLDPRLAKKAKPPEECKENEL
  PYGWEKIDDPIYGTYYVDHINRRTQFENPVLEAKRKLQQHNMPHTELGTKPLQAPGFREKPLFTRDASQL
  KGTFLSTTLKKSNMGFGFTIIGGDEPDEFLQVKSVIPDGPAAQDGKMETGDVIVYINEVCVLGHTHADVV
  KLFQSVPIGQSVNLVLCRGYPLPFDPEDPANSMVPPLAIMERPPPVMVNGRHNYETYLEYISRTSQSVPD
  ITDRPPHSLHSMPTDGQLDGTYPPPVHDDNVSMASSGATQAELMTLTIVKGAQGFGFTIADSPTGQRVKQ
  ILDIQGCPGLCEGDLIVEINQQNVQNLSHTEVVDILKDCPIGSETSLIIHRGGFFSPWKTPKPIMDRWEN
  QGSPQTSLSAPAIPQNLPFPPALHRSSFPDSTEAFDPRKPDPYELYEKSRAIYESRRPDYKELDVHLRRM
  ESGFGFRILGGDEPGQPILIGAVIAMGSADRDGRLHPGDELVYVDGIPVAGKTHRYVIDLMHHAARNGQV
  NLTVRRKVLCGGEPCPENGRSPGSVSTHHSSPRSDYATYTNSNHAAPSSNASPPEGFASHSLQTSDVVIH
  RKENEGFGFVIISSLNRPESGSTITVPHKIGRIIDGSPADRCAKLKVGDRILAVNGQSIINMPHADIVKL
  IKDAGLSVTLRIIPQEELNSPTSAPSSEKQSPMAQQSPLAQQSPLAQPSPATPNSPIAQPAPPQPLQLQG
  HENSYRSEVKARQDVKPDIRQPPFTDYRQPPLDYRQPPGGDYQQPPPLDYRQPPLLDYRQHSPDTRQYPL
  SDYRQPQDFDYFTVDMEKGAKGFGFSIRGGREYKMDLYVLRLAEDGPAIRNGRMRVGDQIIEINGESTRD
  MTHARAIELIKSGGRRVRLLLKRGTGQVPEYDEPAPWSSPAAAAPGLPEVGVSLDDGLAPFSPSHPAPPS
  DPSHQISPGPTWDIKREHDVRKPKELSACGQKKQRLGEQRERSASPQRAARPRLEEAPGGQGRPEAGRPA
  SEARAPGLAAADAADAARAGGKEAPRAAAGSELCRREGPGAAPAFAGPGGGGSGALEAEGRAGARAGPRP
  GPRPPGGAPARKAAVAPGPWKVPGSDKLPSVLKPGASAASR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301128
• ORF Size: 4323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301128.1, NP_001288057.1
• RefSeq Size: 6845
• RefSeq ORF: 4326
• Locus ID: 9863
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• MW: 157.7 kDa
• Gene Summary: The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. This encoded protein is characterized by two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. [provided by RefSeq, Jul 2008]