MCSF Receptor (CSF1R) (NM_005211) Human Tagged ORF Clone
CAT#: RC600012
- TrueORF®
CSF1R (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog, Signal peptide (1-19) plus EC domain (20-517)
"NM_005211" in other vectors (8)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | DDK-His |
Symbol | CSF1R |
Synonyms | C-FMS; CD115; CSF-1R; CSFR; FIM2; FMS; HDLS; M-CSF-R |
Vector | pCMV6-XL5-DDK-His |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>RC600012 representing leader sequence plus the extracellular domain region of NM_005211
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGGGCCCAGGAGTTCTGCTGCTCCTGCTGGTGGCCACAGCTTGGCATGGTCAGGGAATCCCAGTGATAG AGCCCAGTGTCCCTGAGCTGGTCGTGAAGCCAGGAGCAACGGTGACCTTGCGATGTGTGGGCAATGGCAG CGTGGAATGGGATGGCCCCCCATCACCTCACTGGACCCTGTACTCTGATGGCTCCAGCAGCATCCTCAGC ACCAACAACGCTACCTTCCAAAACACGGGGACCTATCGCTGCACTGAGCCTGGAGACCCCCTGGGAGGCA GCGCCGCCATCCACCTCTATGTCAAAGACCCTGCCCGGCCCTGGAACGTGCTAGCACAGGAGGTGGTCGT GTTCGAGGACCAGGACGCACTACTGCCCTGTCTGCTCACAGACCCGGTGCTGGAAGCAGGCGTCTCGCTG GTGCGTGTGCGTGGCCGGCCCCTCATGCGCCACACCAACTACTCCTTCTCGCCCTGGCATGGCTTCACCA TCCACAGGGCCAAGTTCATTCAGAGCCAGGACTATCAATGCAGTGCCCTGATGGGTGGCAGGAAGGTGAT GTCCATCAGCATCCGGCTGAAAGTGCAGAAAGTCATCCCAGGGCCCCCAGCCTTGACACTGGTGCCTGCA GAGCTGGTGCGGATTCGAGGGGAGGCTGCCCAGATCGTGTGCTCAGCCAGCAGCGTTGATGTTAACTTTG ATGTCTTCCTCCAACACAACAACACCAAGCTCGCAATCCCTCAACAATCTGACTTTCATAATAACCGTTA CCAAAAAGTCCTGACCCTCAACCTCGATCAAGTAGATTTCCAACATGCCGGCAACTACTCCTGCGTGGCC AGCAACGTGCAGGGCAAGCACTCCACCTCCATGTTCTTCCGGGTGGTAGAGAGTGCCTACTTGAACTTGA GCTCTGAGCAGAACCTCATCCAGGAGGTGACCGTGGGGGAGGGGCTCAACCTCAAAGTCATGGTGGAGGC CTACCCAGGCCTGCAAGGTTTTAACTGGACCTACCTGGGACCCTTTTCTGACCACCAGCCTGAGCCCAAG CTTGCTAATGCTACCACCAAGGACACATACAGGCACACCTTCACCCTCTCTCTGCCCCGCCTGAAGCCCT CTGAGGCTGGCCGCTACTCCTTCCTGGCCAGAAACCCAGGAGGCTGGAGAGCTCTGACGTTTGAGCTCAC CCTTCGATACCCCCCAGAGGTAAGCGTCATATGGACATTCATCAACGGCTCTGGCACCCTTTTGTGTGCT GCCTCTGGGTACCCCCAGCCCAACGTGACATGGCTGCAGTGCAGTGGCCACACTGATAGGTGTGATGAGG CCCAAGTGCTGCAGGTCTGGGATGACCCATACCCTGAGGTCCTGAGCCAGGAGCCCTTCCACAAGGTGAC GGTGCAGAGCCTGCTGACTGTTGAGACCTTAGAGCACAACCAAACCTACGAGTGCAGGGCCCACAACAGC GTGGGGAGTGGCTCCTGGGCCTTCATACCCATCTCTGCAGGAGCCCACACGCATCCCCCGGATGAGTTCC TCTTCACACCA ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600012 representing signal peptide plus the extracellular domain region of NM_005211
Red=Cloning sites Green= DDK and 6XHIS Tags MGPGVLLLLLVATAWHGQGIPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILS TNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSL VRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPA ELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVA SNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPK LANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTFINGSGTLLCA ASGYPQPNVTWLQCSGHTDRCDEAQVLQVWDDPYPEVLSQEPFHKVTVQSLLTVETLEHNQTYECRAHNS VGSGSWAFIPISAGAHTHPPDEFLFTP TRSGTRSGDYKDDDDKGSHHHHHH |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_005211 |
ORF Size | 1551 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005211.3, NP_005202.2 |
RefSeq Size | 4006 bp |
RefSeq ORF | 2919 bp |
Locus ID | 1436 |
Cytogenetics | 5q32 |
Domains | pkinase, TyrKc, S_TKc, ig, IGc2, IG |
Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
Protein Pathways | Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Pathways in cancer |
MW | 57 kDa |
Gene Summary | 'The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma. [provided by RefSeq, Mar 2017]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116882 | CSF1R (untagged)-Human colony stimulating factor 1 receptor (CSF1R) |
USD 540.00 |
|
SC323543 | CSF1R (untagged)-Kinase deficient mutant (K616M) of Human colony stimulating factor 1 receptor (CSF1R) |
USD 760.00 |
|
RC205288 | CSF1R (Myc-DDK-tagged)-Human colony stimulating factor 1 receptor (CSF1R) |
USD 750.00 |
|
RG205288 | CSF1R (GFP-tagged) - Human colony stimulating factor 1 receptor (CSF1R) |
USD 830.00 |
|
RC205288L1 | Lenti-ORF clone of CSF1R (Myc-DDK-tagged)-Human colony stimulating factor 1 receptor (CSF1R) |
USD 1,176.00 |
|
RC205288L2 | Lenti-ORF clone of CSF1R (mGFP-tagged)-Human colony stimulating factor 1 receptor (CSF1R) |
USD 950.00 |
|
RC205288L3 | Lenti-ORF clone of CSF1R (Myc-DDK-tagged)-Human colony stimulating factor 1 receptor (CSF1R) |
USD 950.00 |
|
RC205288L4 | Lenti-ORF clone of CSF1R (mGFP-tagged)-Human colony stimulating factor 1 receptor (CSF1R) |
USD 950.00 |
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