Eph receptor A6 (EPHA6) (NM_001080448) Human Tagged ORF Clone

CAT#: RC600043

EPHA6 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor A6, transcript variant 1, Signal peptide (1-22) plus EC domain (23-549)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: DDK-His
• Symbol: EPHA6
• Synonyms: EHK-2; EHK2; EK12; EPA6; HEK12; PRO57066
• Vector: pCMV6-XL5-DDK-His
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC600043 representing leader sequence plus the extracellular domain region of NM_001080448
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG
  CGATCGCC
  
  ATGCAATTCCCCTCGCCTCCAGCCGCGAGGAGCTCCCCGGCGCCGCAGGCAGCGTCCTCCTCCGAAGCAG
  CTGCACCTGCAACTGGGCAGCCTGGACCCTCGTGCCCTGTTCCCGGGACCTCGCGCAGGGGGCGCCCCGG
  GACACCCCCTGCGGGCCGGGTGGAGGAGGAAGAGGAGGAGGAGGAAGAAGACGTGGACAAGGACCCCCAT
  CCTACCCAGAACACCTGCCTGCGCTGCCGCCACTTCTCTTTAAGGGAGAGGAAAAGAGAGCCTAGGAGAA
  CCATGGGGGGCTGCGAAGTCCGGGAATTTCTTTTGCAATTTGGTTTCTTCTTGCCTCTGCTGACAGCGTG
  GCCAGGCGACTGCAGTCACGTCTCCAACAACCAAGTTGTGTTGCTTGATACAACAACTGTACTGGGAGAG
  CTAGGATGGAAAACATATCCATTAAATGGGTGGGATGCCATCACTGAAATGGATGAACATAATAGGCCCA
  TTCACACATACCAGGTATGTAATGTAATGGAACCAAACCAAAACAACTGGCTTCGTACAAACTGGATCTC
  CCGTGATGCAGCTCAGAAAATTTATGTGGAAATGAAATTCACACTAAGGGATTGTAACAGCATCCCATGG
  GTCTTGGGGACTTGCAAAGAAACATTTAATCTGTTTTATATGGAATCAGATGAGTCCCACGGAATTAAAT
  TCAAGCCAAACCAGTATACAAAGATCGACACAATTGCTGCTGATGAGAGTTTTACCCAGATGGATTTGGG
  TGATCGCATCCTCAAACTCAACACTGAAATTCGTGAGGTGGGGCCTATAGAAAGGAAAGGATTTTATCTG
  GCTTTTCAAGACATTGGGGCGTGCATTGCCCTGGTTTCAGTCCGTGTTTTCTACAAGAAATGCCCCTTCA
  CTGTTCGTAACTTGGCCATGTTTCCTGATACCATTCCAAGGGTTGATTCCTCCTCTTTGGTTGAAGTACG
  GGGTTCTTGTGTGAAGAGTGCTGAAGAGCGTGACACTCCTAAACTGTATTGTGGAGCTGATGGAGATTGG
  CTGGTTCCTCTTGGAAGGTGCATCTGCAGTACAGGATATGAAGAAATTGAGGGTTCTTGCCATGCTTGCA
  GACCAGGATTCTATAAAGCTTTTGCTGGGAACACAAAATGTTCTAAATGTCCTCCACACAGTTTAACATA
  CATGGAAGCAACTTCTGTCTGTCAGTGTGAAAAGGGTTATTTCCGAGCTGAAAAAGACCCACCTTCTATG
  GCATGTACCAGGCCACCTTCAGCTCCTAGGAATGTGGTTTTTAACATCAATGAAACAGCCCTTATTTTGG
  AATGGAGCCCACCAAGTGACACAGGAGGGAGAAAAGATCTCACATACAGTGTAATCTGTAAGAAATGTGG
  CTTAGACACCAGCCAGTGTGAGGACTGTGGTGGAGGACTCCGCTTCATCCCAAGACATACAGGCCTGATC
  AACAATTCCGTGATAGTACTTGACTTTGTGTCTCACGTGAATTACACCTTTGAAATAGAAGCAATGAATG
  GAGTTTCTGAGTTGAGTTTTTCTCCCAAGCCATTCACAGCTATTACAGTGACCACGGATCAAGATGCACC
  TTCCCTGATAGGTGTGGTAAGGAAGGACTGGGCATCC
  
  ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC
  TGGTACCGATATCAAGCTTGTCGACTCTAGA
  Protein Sequence

  >RC600043 representing signal peptide plus the extracellular domain region of NM_001080448
  Red=Cloning sites Green= DDK and 6XHIS Tags
  
  MQFPSPPAARSSPAPQAASSSEAAAPATGQPGPSCPVPGTSRRGRPGTPPAGRVEEEEEEEEEDVDKDPH
  PTQNTCLRCRHFSLRERKREPRRTMGGCEVREFLLQFGFFLPLLTAWPGDCSHVSNNQVVLLDTTTVLGE
  LGWKTYPLNGWDAITEMDEHNRPIHTYQVCNVMEPNQNNWLRTNWISRDAAQKIYVEMKFTLRDCNSIPW
  VLGTCKETFNLFYMESDESHGIKFKPNQYTKIDTIAADESFTQMDLGDRILKLNTEIREVGPIERKGFYL
  AFQDIGACIALVSVRVFYKKCPFTVRNLAMFPDTIPRVDSSSLVEVRGSCVKSAEERDTPKLYCGADGDW
  LVPLGRCICSTGYEEIEGSCHACRPGFYKAFAGNTKCSKCPPHSLTYMEATSVCQCEKGYFRAEKDPPSM
  ACTRPPSAPRNVVFNINETALILEWSPPSDTGGRKDLTYSVICKKCGLDTSQCEDCGGGLRFIPRHTGLI
  NNSVIVLDFVSHVNYTFEIEAMNGVSELSFSPKPFTAITVTTDQDAPSLIGVVRKDWAS
  
  TRSGTRSGDYKDDDDKGSHHHHHH
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_001080448
• ORF Size: 1647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001080448.2, NP_001073917.2
• RefSeq Size: 3677
• RefSeq ORF: 3393
• Locus ID: 285220
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 61.1 kDa
• Gene Summary: Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. [UniProtKB/Swiss-Prot Function]