PPME1 (NM_016147) Human Tagged ORF Clone

CAT#: RG200009

  • TrueORF®

PPME1 (GFP-tagged) - Human protein phosphatase methylesterase 1 (PPME1)


  "NM_016147" in other vectors (7)

Reconstitution Protocol

USD 460.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol PPME1
Synonyms ABDH19; PME-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG200009 representing NM_016147
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCCCTCGAAAAGAGCATGCACCTCGGCCGCCTTCCCTCTCGCCCACCTCTACCCGGCAGCGGGG
GCAGTCAGAGCGGAGCCAAGATGCGAATGGGCCCTGGAAGAAAGCGGGACTTTTCCCCTGTTCCTTGGAG
TCAGTATTTTGAGTCCATGGAAGATGTAGAAGTAGAGAATGAAACTGGCAAGGATACTTTTCGAGTCTAC
AAGAGTGGTTCAGAGGGTCCAGTCCTGCTCCTTCTGCATGGAGGAGGTCATTCTGCCCTTTCTTGGGCTG
TGTTCACGGCAGCGATTATTAGTAGAGTTCAGTGTAGGATTGTAGCTTTGGATCTGCGAAGTCATGGTGA
AACAAAGGTCAAGAATCCTGAAGATCTGTCTGCAGAAACAATGGCAAAAGACGTTGGCAATGTGGTTGAA
GCCATGTATGGGGACCTTCCTCCTCCAATTATGCTGATTGGACATAGCATGGGTGGTGCTATTGCAGTCC
ACACAGCATCATCCAACCTGGTACCAAGCCTCTTGGGTCTGTGCATGATTGATGTTGTAGAAGGTACAGC
TATGGATGCACTTAATAGCATGCAGAATTTCTTACGGGGTCGTCCTAAAACCTTCAAGTCTCTGGAGAAT
GCTATTGAATGGAGTGTGAAGAGTGGCCAGATTCGAAATCTGGAGTCTGCCCGTGTCTCAATGGTTGGCC
AAGTCAAACAGTGTGAAGGAATTACAAGTCCAGAAGGCTCAAAATCTATAGTGGAAGGAATCATAGAGGA
AGAAGAAGAAGATGAGGAAGGAAGTGAGTCTATAAGCAAGAGGAAAAAGGAAGATGACATGGAGACCAAG
AAAGACCATCCATACACCTGGAGAATTGAACTGGCAAAAACAGAAAAATACTGGGACGGCTGGTTCCGAG
GCTTATCCAATCTCTTTCTTAGTTGTCCCATTCCTAAATTGCTGCTCTTGGCTGGTGTTGATAGATTGGA
TAAAGATCTGACCATTGGCCAGATGCAAGGGAAGTTCCAGATGCAGGTCCTACCCCAGTGTGGCCATGCA
GTCCATGAGGATGCCCCTGACAAGGTAGCTGAAGCTGTTGCCACTTTCCTGATCCGGCACAGGTTTGCAG
AACCCATCGGTGGATTCCAGTGTGTGTTTCCTGGCTGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG200009 representing NM_016147
Red=Cloning site Green=Tags(s)

MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVY
KSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVE
AMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLEN
AIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETK
KDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHA
VHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016147
ORF Size 1158 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_016147.1, NP_057231.1
RefSeq Size 2484
RefSeq ORF 1161
Locus ID 51400
Protein Families Druggable Genome
Gene Summary This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

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