EIF2AK1 (NM_014413) Human Tagged ORF Clone

CAT#: RG200065

EIF2AK1 (GFP-tagged) - Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EIF2AK1
• Synonyms: HCR; HRI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200065 representing NM_014413
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGGGGCAACTCCGGGGTCCGCAAGCGCGAAGAGGAGGGCGACGGGGCTGGGGCTGTGGCTGCGC
  CGCCGGCCATCGACTTTCCCGCCGAGGGCCCGGACCCCGAATATGACGAATCTGATGTTCCAGCAGAAAT
  CCAGGTGTTAAAAGAACCCCTACAACAGCCAACCTTCCCTTTTGCAGTTGCAAACCAACTCTTGCTGGTT
  TCTTTGCTGGAGCACTTGAGCCACGTGCATGAACCAAACCCACTTCGTTCAAGACAGGTGTTTAAGCTAC
  TTTGCCAGACGTTTATCAAAATGGGGCTGCTGTCTTCTTTCACTTGTAGTGACGAGTTTAGCTCATTGAG
  ACTACATCACAACAGAGCTATTACTCACTTAATGAGGTCTGCTAAAGAGAGAGTTCGTCAGGATCCTTGT
  GAGGATATTTCTCGTATCCAGAAAATCAGATCAAGGGAAGTAGCCTTGGAAGCACAAACTTCACGTTACT
  TAAATGAATTTGAAGAACTTGCCATCTTAGGAAAAGGTGGATACGGAAGAGTATACAAGGTCAGGAATAA
  ATTAGATGGTCAGTATTATGCAATAAAAAAAATCCTGATTAAGGGTGCAACTAAAACAGTTTGCATGAAG
  GTCCTACGGGAAGTGAAGGTGCTGGCAGGTCTTCAGCACCCCAATATTGTTGGCTATCACACCGCGTGGA
  TAGAACATGTTCATGTGATTCAGCCACGAGCAGACAGAGCTGCCATTGAGTTGCCATCTCTGGAAGTGCT
  CTCCGACCAGGAAGAGGACAGAGAGCAATGTGGTGTTAAAAATGATGAAAGTAGCAGCTCATCCATTATC
  TTTGCTGAGCCCACCCCAGAAAAAGAAAAACGCTTTGGAGAATCTGACACTGAAAATCAGAATAACAAGT
  CGGTGAAGTACACCACCAATTTAGTCATAAGAGAATCTGGTGAACTTGAGTCGACCCTGGAGCTCCAGGA
  AAATGGCTTGGCTGGTTTGTCTGCCAGTTCAATTGTGGAACAGCAGCTGCCACTCAGGCGTAATTCCCAC
  CTAGAGGAGAGTTTCACATCCACCGAAGAATCTTCCGAAGAAAATGTCAACTTTTTGGGTCAGACAGAGG
  CACAGTACCACCTGATGCTGCACATCCAGATGCAGCTGTGTGAGCTCTCGCTGTGGGATTGGATAGTCGA
  GAGAAACAAGCGGGGCCGGGAGTATGTGGACGAGTCTGCCTGTCCTTATGTTATGGCCAATGTTGCAACA
  AAAATTTTTCAAGAATTGGTAGAAGGTGTGTTTTACATACATAACATGGGAATTGTGCACCGAGATCTGA
  AGCCAAGAAATATTTTTCTTCATGGCCCTGATCAGCAAGTAAAAATAGGAGACTTTGGTCTGGCCTGCAC
  AGACATCCTACAGAAGAACACAGACTGGACCAACAGAAACGGGAAGAGAACACCAACACATACGTCCAGA
  GTGGGTACTTGTCTGTACGCTTCACCCGAACAGTTGGAAGGATCTGAGTATGATGCCAAGTCAGATATGT
  ACAGCTTGGGTGTGGTCCTGCTAGAGCTCTTTCAGCCGTTTGGAACAGAAATGGAGCGAGCAGAAGTTCT
  AACAGGTTTAAGAACTGGTCAGTTGCCGGAATCCCTCCGTAAAAGGTGTCCAGTGCAAGCCAAGTATATC
  CAGCACTTAACGAGAAGGAACTCATCGCAGAGACCATCTGCCATTCAGCTGCTGCAGAGTGAACTTTTCC
  AAAATTCTGGAAATGTTAACCTCACCCTACAGATGAAGATAATAGAGCAAGAAAAAGAAATTGCAGAACT
  AAAGAAGCAGCTAAACCTCCTTTCTCAAGACAAAGGGGTGAGGGATGACGGAAAGGATGGGGGCGTGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200065 representing NM_014413
  Red=Cloning site Green=Tags(s)
  
  MQGGNSGVRKREEEGDGAGAVAAPPAIDFPAEGPDPEYDESDVPAEIQVLKEPLQQPTFPFAVANQLLLV
  SLLEHLSHVHEPNPLRSRQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPC
  EDISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMK
  VLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSII
  FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSH
  LEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVAT
  KIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSR
  VGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYI
  QHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014413
• ORF Size: 1890 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014413.2, NP_055228.2
• RefSeq Size: 4465
• RefSeq ORF: 1893
• Locus ID: 27102
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]