ASF1A (NM_014034) Human Tagged ORF Clone

CAT#: RG200324

ASF1A (GFP-tagged) - Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASF1A
• Synonyms: CGI-98; CIA; HSPC146
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200324 representing NM_014034
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGGTTCAGGTGAACAATGTAGTGGTGCTGGATAACCCTTCTCCTTTCTACAACCCGTTCCAGT
  TCGAGATCACCTTCGAGTGCATCGAGGACCTGTCTGAAGACTTGGAATGGAAAATTATCTATGTGGGCTC
  TGCAGAAAGTGAAGAATACGATCAAGTTTTAGACTCTGTTTTAGTGGGTCCTGTTCCCGCAGGAAGGCAT
  ATGTTTGTATTTCAGGCTGATGCACCTAATCCAGGACTCATTCCAGATGCAGATGCAGTAGGCGTAACTG
  TTGTGCTAATTACTTGTACCTATCGAGGACAAGAATTTATTAGAGTTGGCTATTATGTAAATAATGAATA
  TACTGAGACAGAATTAAGGGAAAATCCACCAGTAAAACCAGACTTTTCTAAGCTTCAAAGGAATATTTTG
  GCATCTAATCCCAGGGTCACAAGATTCCACATTAATTGGGAAGATAACACAGAAAAACTGGAAGATGCAG
  AGAGCAGTAATCCAAATCTACAGTCACTTCTTTCAACAGATGCATTACCTTCAGCATCAAAGGGATGGTC
  CACATCAGAAAACTCACTAAATGTCATGTTAGAATCCCACATGGACTGCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200324 representing NM_014034
  Red=Cloning site Green=Tags(s)
  
  MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRH
  MFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNIL
  ASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014034
• ORF Size: 612 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014034.2, NP_054753.1
• RefSeq Size: 2525
• RefSeq ORF: 615
• Locus ID: 25842
• Domains: Anti-silence
• Gene Summary: This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008]