BLMH (NM_000386) Human Tagged ORF Clone

CAT#: RG200393

BLMH (GFP-tagged) - Human bleomycin hydrolase (BLMH)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BLMH
• Synonyms: BH; BMH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200393 representing NM_000386
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCTCGGGACTGAATTCGGAGAAGGTAGCTGCTCTGATACAGAAACTGAATTCCGACCCCCAGT
  TCGTACTTGCCCAGAATGTCGGGACCACCCACGACCTGCTGGACATCTGTCTGAAGCGGGCCACGGTGCA
  GCGCGCGCAGCATGTGTTCCAGCACGCCGTGCCCCAGGAGGGCAAGCCAATCACCAACCAGAAGAGCTCA
  GGGCGATGCTGGATCTTTTCTTGTCTGAATGTTATGAGGCTTCCATTCATGAAAAAGTTAAATATTGAAG
  AATTTGAGTTTAGCCAATCTTACCTGTTTTTTTGGGACAAGGTTGAACGCTGTTATTTCTTCTTGAGTGC
  TTTTGTGGACACAGCCCAGAGAAAGGAGCCTGAGGATGGGAGGCTGGTGCAGTTTTTGCTTATGAACCCT
  GCAAATGATGGTGGCCAATGGGATATGCTTGTTAATATTGTTGAAAAATATGGTGTTATCCCTAAGAAAT
  GCTTCCCTGAATCTTATACAACAGAGGCAACCAGAAGGATGAATGATATTCTGAATCACAAGATGAGAGA
  ATTCTGTATACGACTGCGGAACCTGGTACACAGTGGAGCAACCAAAGGAGAAATCTCGGCCACACAGGAC
  GTCATGATGGAGGAGATATTCCGAGTGGTGTGCATCTGTTTGGGTAATCCACCAGAGACATTCACCTGGG
  AATATCGAGACAAAGATAAAAATTATCAGAAAATTGGCCCCATAACACCCTTGGAGTTTTACAGGGAACA
  TGTCAAGCCACTCTTCAATATGGAAGATAAGATTTGTTTAGTGAATGACCCTAGGCCCCAGCACAAGTAC
  AACAAACTTTACACAGTGGAATACTTAAGCAATATGGTTGGAGGGAGAAAAACTCTATACAACAACCAGC
  CCATTGACTTCCTGAAAAAGATGGTTGCTGCCTCCATCAAAGATGGAGAGGCTGTGTGGTTTGGCTGTGA
  TGTTGGAAAACACTTCAATAGCAAGCTGGGCCTCAGTGACATGAATCTCTATGACCATGAGTTAGTGTTT
  GGTGTCTCCTTGAAGAACATGAATAAAGCGGAGAGGCTGACTTTTGGTGAGTCACTTATGACCCACGCCA
  TGACCTTCACTGCTGTCTCAGAGAAGGATGATCAGGATGGTGCTTTCACAAAATGGAGAGTGGAGAATTC
  ATGGGGTGAAGACCATGGCCACAAAGGTTACCTGTGCATGACAGATGAGTGGTTCTCTGAGTATGTCTAC
  GAAGTGGTGGTGGACAGGAAGCATGTCCCTGAAGAGGTGCTAGCTGTGTTAGAGCAGGAACCCATTATCC
  TGCCAGCATGGGACCCCATGGGAGCTTTGGCTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200393 representing NM_000386
  Red=Cloning site Green=Tags(s)
  
  MSSSGLNSEKVAALIQKLNSDPQFVLAQNVGTTHDLLDICLKRATVQRAQHVFQHAVPQEGKPITNQKSS
  GRCWIFSCLNVMRLPFMKKLNIEEFEFSQSYLFFWDKVERCYFFLSAFVDTAQRKEPEDGRLVQFLLMNP
  ANDGGQWDMLVNIVEKYGVIPKKCFPESYTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISATQD
  VMMEEIFRVVCICLGNPPETFTWEYRDKDKNYQKIGPITPLEFYREHVKPLFNMEDKICLVNDPRPQHKY
  NKLYTVEYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNSKLGLSDMNLYDHELVF
  GVSLKNMNKAERLTFGESLMTHAMTFTAVSEKDDQDGAFTKWRVENSWGEDHGHKGYLCMTDEWFSEYVY
  EVVVDRKHVPEEVLAVLEQEPIILPAWDPMGALAE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000386
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_000386.2, NP_000377.1
• RefSeq Size: 2417
• RefSeq ORF: 1368
• Locus ID: 642
• Domains: Pept_C1-like
• Protein Families: Druggable Genome, Protease
• Gene Summary: Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]