BLMH (NM_000386) Human Untagged Clone

CAT#: SC320216

BLMH (untagged)-Human bleomycin hydrolase (BLMH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BLMH
• Synonyms: BH; BMH
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000386.2 GACAGGTCGGGCCCCCTAGGCAGCGAGCCGCAGCGCAATCCCGGCGCTCGCCCAAGGACC
  CTGGAAGCTACCGTTACCCCGCCGGGCAGCGTGGGCGCCATGAGCAGCTCGGGACTGAAT
  TCGGAGAAGGTAGCTGCTCTGATACAGAAACTGAATTCCGACCCCCAGTTCGTACTTGCC
  CAGAATGTCGGGACCACCCACGACCTGCTGGACATCTGTCTGAAGCGGGCCACGGTGCAG
  CGCGCGCAGCATGTGTTCCAGCACGCCGTGCCCCAGGAGGGCAAGCCAATCACCAACCAG
  AAGAGCTCAGGGCGATGCTGGATCTTTTCTTGTCTGAATGTTATGAGGCTTCCATTCATG
  AAAAAGTTAAATATTGAAGAATTTGAGTTTAGCCAATCTTACCTGTTTTTTTGGGACAAG
  GTTGAACGCTGTTATTTCTTCTTGAGTGCTTTTGTGGACACAGCCCAGAGAAAGGAGCCT
  GAGGATGGGAGGCTGGTGCAGTTTTTGCTTATGAACCCTGCAAATGATGGTGGCCAATGG
  GATATGCTTGTTAATATTGTTGAAAAATATGGTGTTATCCCTAAGAAATGCTTCCCTGAA
  TCTTATACAACAGAGGCAACCAGAAGGATGAATGATATTCTGAATCACAAGATGAGAGAA
  TTCTGTATACGACTGCGGAACCTGGTACACAGTGGAGCAACCAAAGGAGAAATCTCGGCC
  ACACAGGACGTCATGATGGAGGAGATATTCCGAGTGGTGTGCATCTGTTTGGGTAATCCA
  CCAGAGACATTCACCTGGGAATATCGAGACAAAGATAAAAATTATCAGAAAATTGGCCCC
  ATAACACCCTTGGAGTTTTACAGGGAACATGTCAAGCCACTCTTCAATATGGAAGATAAG
  ATTTGTTTAGTGAATGACCCTAGGCCCCAGCACAAGTACAACAAACTTTACACAGTGGAA
  TACTTAAGCAATATGGTTGGAGGGAGAAAAACTCTATACAACAACCAGCCCATTGACTTC
  CTGAAAAAGATGGTTGCTGCCTCCATCAAAGATGGAGAGGCTGTGTGGTTTGGCTGTGAT
  GTTGGAAAACACTTCAATAGCAAGCTGGGCCTCAGTGACATGAATCTCTATGACCATGAG
  TTAGTGTTTGGTGTCTCCTTGAAGAACATGAATAAAGCGGAGAGGCTGACTTTTGGTGAG
  TCACTTATGACCCACGCCATGACCTTCACTGCTGTCTCAGAGAAGGATGATCAGGATGGT
  GCTTTCACAAAATGGAGAGTGGAGAATTCATGGGGTGAAGACCATGGCCACAAAGGTTAC
  CTGTGCATGACAGATGAGTGGTTCTCTGAGTATGTCTACGAAGTGGTGGTGGACAGGAAG
  CATGTCCCTGAAGAGGTGCTAGCTGTGTTAGAGCAGGAACCCATTATCCTGCCAGCATGG
  GACCCCATGGGAGCTTTGGCTGAGTGATACTGCCCTCCAGCTCTTTCCTCCTTCCATGGA
  ACCTGACGTAGCTGCAAAGGACAGATCCAGGGACTGAAGCCAAAGTTATGCAAGGGACTG
  TGTGTTGCCACAGGACACAGTCAGATTTCCAGTCTCCACCAGGAACCTCTTCAGAAAGTG
  TGCTTTATGCTGAAACAGAATACTGTTAAAGGAAAAAAAAGAGGGGGGAAGATCAGGTCA
  TACTATCTACTCTCCTCATCTCTAACAGCTCAGGATCTCTTAGCATTTTAATTAGATGTA
  ATTGTTTGTCTTTAACTGTCAAAAAGTTTGGTTCTGTGTCTGTGTTTTAATAAGACGAGA
  GGACGAGCGATTGAGGTGTATGGAGAGAAAACAGACCTAATGCTCCTTGTTCCTAGAGTA
  GAGTGGAGGGAGGGTGGCCTAAGAGTTGAGCTCTCGGAACTGCATGCTGCTGGACAGTAT
  CACTGTCTTTCCTAGATGGCAGTCACTGAATTCCATTTTTTCAAGGTAATTTCTTGTGCC
  TCTAATAGCCCAAGAATGGGAGGTTGATCAGATCTGACATGATTCCTTCCTGTTCTGAAC
  TGTGGGGTGTGCACATCTCTGCTTGAGTCAGGTTTGAGTAGAGGCTTAGAGACAGTTGGG
  TGAGAACAACCAAAATCTTATCATGGTCTCAGTCATAATCATTAGGGGGAACTCTAGCCA
  AATGGTTTAACTTCTGCCTGTGGAACTGGGGATTGGGTGGGCAGGAAAAGGTGATATCCA
  TTCTTTCTGATAACTAGATGGTGCTGAGAAGCTTTTGAATAAAAACTTTGCTAAATGAGA
  AAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000386.2 unedited
  GGGCACTGTGTAGTACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTAC
  CGAGGAGATCTGCCGCCGCGATCGCGGCGCGCCAGATCTCAAGCTTAACTAGTTAGCGGA
  CCGACGCGTTAAGCGGCCGCGAATTCAGATCCACAAGTTTGTACAAAAAAGCAGGCTTGT
  AAAACGACGGCCAGTAACTATAACGGTCCTAAGGTAGCGAGGCCTGGGTGGCGAATTCGG
  CACGAGGGACAGGTCGGGCCCCCTAGGCAGCGAGCCGCAGCGCAATCCCGGCGCTCGCCC
  AAGGACCCTGGAAGCTACCGTTACCCCGCCGGGCAGCGTGGGCGCCATGAGCAGCTCGGG
  ACTGAATTCGGAGAAGGTAGCTGCTCTGATACAGAAACTGAATTCCGACCCCCAGTTCGT
  ACTTGCCCAGAATGTCGGGACCACCCACGACCTGCTGGACATCTGTCTGAAGCGGGCCAC
  GGTGCAGCGCGCGCAGCATGTGTTCCAGCACGCCGTGCCCCAGGAGGGCAAGCCAATCAC
  CAACCAGAAGAGCTCAGGGCGATGCTGGATCTTTTCTTGTCTGAATGTTATGAGGCTTCC
  ATTCATGAAAAAGTTAAATATTGAAGAATTTGAGTTTAGCCAATCTTACCTGTTTTTTTG
  GGACAAGGTTGAACGCTGTTATTTCTTCTTGAGTGCTTTTGTGGACACAGCCCAGAGAAA
  GGAGCCTGAGATGGGAGGCTGGTGCAGTTTTTGCTTATGAACCCTGCAATGATGGTGGCC
  CAATGGGATATGCTTGTAATATGTGAAAAAATATGGTGTTATCCCTAGAATGCTCCCTGA
  TCTATACAACAGAGCACCAGAGATGATGATATCTGATCACAGATGAGAGAATTCTGTATA
  CGACTGCGACTGTACACAGTGGAGCACCAAGGAAAATTCTCGCCACACCAGACGTCATGA
  TGGAGGAAATTTTCCGAGTGGGGGTGG
• Restriction Sites: Please inquire
• ACCN: NM_000386
• ORF Size: 1368 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_000386.2, NP_000377.1
• RefSeq Size: 2417
• RefSeq ORF: 1368
• Locus ID: 642
• Domains: Pept_C1-like
• Protein Families: Druggable Genome, Protease
• Gene Summary: Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]