CLK3 (NM_003992) Human Tagged ORF Clone

CAT#: RG200489

CLK3 (GFP-tagged) - Human CDC-like kinase 3 (CLK3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CLK3
• Synonyms: PHCLK3; PHCLK3/152
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200489 representing NM_003992
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGGCGCGCCC
  
  ATGCATCACTGTAAGCGATACCGCTCCCCTGAACCAGACCCGTACCTGAGCTACCGATGGAAGAGGAGGA
  GGTCCTACAGTCGGGAACATGAAGGGAGACTGCGATACCCGTCCCGAAGGGAGCCTCCCCCACGAAGATC
  TCGGTCCAGAAGCCATGACCGCCTGCCCTACCAGAGGAGGTACCGGGAGCGCCGTGACAGCGATACATAC
  CGGTGTGAAGAGCGGAGCCCATCCTTTGGAGAGGACTACTATGGACCTTCACGTTCTCGTCATCGTCGGC
  GATCGCGGGAGAGGGGGCCATACCGGACCCGCAAGCATGCCCACCACTGCCACAAACGCCGCACCAGGTC
  TTGTAGCAGCGCCTCCTCGAGAAGCCAACAGAGCAGTAAGCGCACAGGCCGGAGTGTGGAAGATGACAAG
  GAGGGTCACCTGGTGTGCCGGATCGGCGATTGGCTCCAAGAGCGATATGAGATTGTGGGGAACCTGGGTG
  AAGGCACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGGGAAGTCTCAGGTTGCCCTGAAGAT
  CATCCGCAACGTGGGCAAGTACCGGGAGGCTGCCCGGCTAGAAATCAACGTGCTCAAAAAAATCAAGGAG
  AAGGACAAAGAAAACAAGTTCCTGTGTGTCTTGATGTCTGACTGGTTCAACTTCCACGGTCACATGTGCA
  TCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAGGAGAATAACTTCCAGCCTTACCCCCT
  ACCACATGTCCGGCACATGGCCTACCAGCTCTGCCACGCCCTTAGATTTCTGCATGAGAATCAGCTGACC
  CATACAGACTTGAAACCTGAGAACATCCTGTTTGTGAATTCTGAGTTTGAAACCCTCTACAATGAGCACA
  AGAGCTGTGAGGAGAAGTCAGTGAAGAACACCAGCATCCGAGTGGCTGACTTTGGCAGTGCCACATTTGA
  CCATGAGCACCACACCACCATTGTGGCCACCCGTCACTATCGCCCGCCTGAGGTGATCCTTGAGCTGGGC
  TGGGCACAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTTTGAGTACTACCGGGGCTTCACACTCT
  TCCAGACCCACGAAAACCGAGAGCACCTGGTGATGATGGAGAAGATCCTAGGGCCCATCCCATCACACAT
  GATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTAGTTTGGGATGAGAACAGCTCTGAC
  GGCCGGTATGTGAAGGAGAACTGCAAACCTCTGAAGAGTTACATGCTCCAAGACTCCCTGGAGCACGTGC
  AGCTGTTTGACCTGATGAGGAGGATGTTAGAATTTGACCCTGCCCAGCGCATCACACTGGCCGAGGCCCT
  GCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTCCTTCCACACCAGCCGCAACCCAAGCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200489 representing NM_003992
  Red=Cloning site Green=Tags(s)
  
  MHHCKRYRSPEPDPYLSYRWKRRRSYSREHEGRLRYPSRREPPPRRSRSRSHDRLPYQRRYRERRDSDTY
  RCEERSPSFGEDYYGPSRSRHRRRSRERGPYRTRKHAHHCHKRRTRSCSSASSRSQQSSKRTGRSVEDDK
  EGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKE
  KDKENKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHMAYQLCHALRFLHENQLT
  HTDLKPENILFVNSEFETLYNEHKSCEEKSVKNTSIRVADFGSATFDHEHHTTIVATRHYRPPEVILELG
  WAQPCDVWSIGCILFEYYRGFTLFQTHENREHLVMMEKILGPIPSHMIHRTRKQKYFYKGGLVWDENSSD
  GRYVKENCKPLKSYMLQDSLEHVQLFDLMRRMLEFDPAQRITLAEALLHPFFAGLTPEERSFHTSRNPSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003992
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003992.1, NP_003983.1
• RefSeq Size: 1863 bp
• RefSeq ORF: 1473 bp
• Locus ID: 1198
• Cytogenetics: 15q24.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. [provided by RefSeq, Jul 2008]'