CLK3 (NM_003992) Human Untagged Clone

CAT#: SC323354

CLK3 (untagged)-Kinase deficient mutant (K186M) of Human CDC-like kinase 3 (CLK3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLK3
• Synonyms: PHCLK3; PHCLK3/152
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003992, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCATCACTGTAAGCGATACCGCTCCCCTGAACCAGACCCGTACCTGAGCTACCGATGGAAGAGGAGGA
  GGTCCTACAGTCGGGAACATGAAGGGAGACTGCGATACCCGTCCCGAAGGGAGCCTCCCCCACGAAGATC
  TCGGTCCAGAAGCCATGACCGCCTGCCCTACCAGAGGAGGTACCGGGAGCGCCGTGACAGCGATACATAC
  CGGTGTGAAGAGCGGAGCCCATCCTTTGGAGAGGACTACTATGGACCTTCACGTTCTCGTCATCGTCGGC
  GATCGCGGGAGAGGGGGCCATACCGGACCCGCAAGCATGCCCACCACTGCCACAAACGCCGCACCAGGTC
  TTGTAGCAGCGCCTCCTCGAGAAGCCAACAGAGCAGTAAGCGCAGCAGCCGGAGTGTGGAAGATGACAAG
  GAGGGTCACCTGGTGTGCCGGATCGGCGATTGGCTCCAAGAGCGATATGAGATTGTGGGGAACCTGGGTG
  AAGGCACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGGGAAGTCTCAGGTTGCCCTGAAGAT
  CATCCGCAACGTGGGCAAGTACCGGGAGGCTGCCCGGCTAGAAATCAACGTGCTCAAAAAAATCAAGGAG
  AAGGACAAAGAAAACAAGTTCCTGTGTGTCTTGATGTCTGACTGGTTCAACTTCCACGGTCACATGTGCA
  TCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAGGAGAATAACTTCCAGCCTTACCCCCT
  ACCACATGTCCGGCACATGGCCTACCAGCTCTGCCACGCCCTTAGATTTCTGCATGAGAATCAGCTGACC
  CATACAGACTTGAAACCAGAGAACATCCTGTTTGTGAATTCTGAGTTTGAAACCCTCTACAATGAGCACA
  AGAGCTGTGAGGAGAAGTCAGTGAAGAACACCAGCATCCGAGTGGCTGACTTTGGCAGTGCCACATTTGA
  CCATGAGCACCACACCACCATTGTGGCCACCCGTCACTATCGCCCGCCTGAGGTGATCCTTGAGCTGGGC
  TGGGCACAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTTTGAGTACTACCGGGGCTTCACACTCT
  TCCAGACCCACGAAAACCGAGAGCACCTGGTGATGATGGAGAAGATCCTAGGGCCCATCCCATCACACAT
  GATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTAGTTTGGGATGAGAACAGCTCTGAC
  GGCCGGTATGTGAAGGAGAACTGCAAACCTCTGAAGAGTTACATGCTCCAAGACTCCCTGGAGCACGTGC
  AGCTGTTTGACCTGATGAGGAGGATGTTAGAATTTGACCCTGCCCAGCGCATCACACTGGCCGAGGCCCT
  GCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTCCTTCCACACCAGCCGCAACCCAAGCAGA
  TGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003992 unedited
  CCGCCCGTCTGAGCAATGGGCGGTAGGCGTGTACGGATGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGTCTCCACCT
  CTGCCTTTGACTGACACCTCAATCTGTCAATCGGAACCGCCTACCATGCGATTGGTCGGATGGCGTTTCG
  GGGGGCGGTGCATGCAGAGAAGCCAACAGAGCAGTAAGCGCAGCAGCCGGAGTGTGGAAGATGACAAGGA
  GGGTCACCTGGTGTGCCGGATCGGCGATTGGCTCCAAGAGCGATGTACAGGCCACCCTTTCGTAAGACTG
  TTAGCCTCTTCTACTTTCTACCCCCCCTTGTTAAAACGAAGAACCTCTTCCCTGGCCCTGGAAGGGGCCT
  CCTAGTCGCCCGGTGGTGGCCTTACCCGGGGCCCCCAGTAATGGGCCTGATGGAACACAAACCCTAGCAA
  CCTTCAATTTTTTAAAAGGGTACCATGGGAAAAAAAAACCTTTTTTGGTTTTTTGAAAATTTAAATTGCG
  GGGACTTGGGCGGGGATGGGCAACCCTGGGCTTAAAAATTCAAGGCCCAATTTTTTTTTCTTCCCTAAAG
  AATTTGGGGGGAACCCGGGGTAAAGGGCACCTTTGGCAGGGGGGGGAGAGTTTTGACCCACCCCAAAGGG
  AATCTCTAGGTGTCCCCAGGAGAATCACGCAAGTTGGCGCATATTCGCGGGGGGGCCCCGCGTGTAAAAC
  TCCCTGCTGCTAAAAATCACGAGGAGGAAGCACAAAACAATTTTTTTTGTGGAGAGATCATTGAGAAAAC
  ACCCTCCTCTGGGTGGCTTTTGTGTGGCCCCCTTTTATCACAGAGCACACACACTTGTGTGCACCGTCCG
  TATCTCCCTGAGTGATACTCTAGCTGGCGGGCGCAACAGGAGCTCAGTGATAGCGAGCTCTTCGATATAA
  CAGCCGTCATCCGTCGTACAGCGCAGCACGTAGCCGACGTCG
  
  Kinase domain sequence

  >SC323354 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSACTGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGTCTCCACCTCTGCCTT
  TGACTGACACCTCAATCTGTCAATCGGAACCGCCTACCATGCGATTGGTCGGATGGCGTTTCGGGGGGCG
  GTGCATGCAGAGAAGCCAACAGAGCAGTAAGCGCAGCAGCCGGAG
  
• Restriction Sites: Please inquire
• ACCN: NM_003992
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003992.1, NP_003983.1
• RefSeq Size: 1762 bp
• RefSeq ORF: 1473 bp
• Locus ID: 1198
• Cytogenetics: 15q24.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.