Calpain 6 (CAPN6) (NM_014289) Human Tagged ORF Clone

CAT#: RG200539

CAPN6 (GFP-tagged) - Human calpain 6 (CAPN6)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CAPN6
• Synonyms: CalpM; CANPX; CAPNX; DJ914P14.1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200539 representing NM_014289
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGCATCAAAGACA
  GCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTCTACAACCGACTGCTTCCTGG
  AAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGACCCCCATCTGATTGTGGGCAACATTAGCAAC
  CACCAGCTGACCCAAGGGAGACTGGGGCACAAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGG
  AGTCTCATTGGACAAAGACAATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATA
  CGCTGGGATATTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
  CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAATGCTCTGCTGG
  AAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTGACCATCACTGATATTATTGT
  GGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAGAAAGGAAGATACACTGAGCTTGTTGAGGAG
  AAGTACAAGCTATTCGGAGAACTGTACAAAACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGT
  CTCCCAATCAGGAGGAGCAAGAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGAC
  TGATATTCGCAAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
  CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAAGAGTGGCAGC
  AACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGATGGAGAGTTTTGGATGAGCTT
  GGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGCCGCAATGTGAACAACCCTATTTTTGGCCGA
  AAGGAGCTGGAATCGGTGTTGGGATGCTGGACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCT
  GCTATAACAACCGTGATACCTTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAA
  GGTCATTATGTCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
  ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTACATCCAGGAGC
  GTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAGTACCTGAAGAAGGGCAACTA
  TGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGCGAGTTTCTCCTGAGAATCTTCTCTGAAGTG
  CCTGTCCAGCTCAGGGAACTGACTCTGGACATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACC
  CGAAAGTAGTTACTCAGATCACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGT
  AAACCCATATTTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
  GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTACAGGTCTGGA
  ACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCTGACCCCAGCGACTGCCGTGA
  TCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACTGCCAAAGTCAAGCAAGGCCACATCAGCTTC
  AAGGTTATTTCCAGCGATGATCTCACTGAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200539 representing NM_014289
  Red=Cloning site Green=Tags(s)
  
  MGPPLKLFKNQKYQELKQECIKDSRLFCDPTFLPENDSLFYNRLLPGKVVWKRPQDICDDPHLIVGNISN
  HQLTQGRLGHKPMVSAFSCLAVQESHWTKTIPNHKEQEWDPQKTEKYAGIFHFRFWHFGEWTEVVIDDLL
  PTINGDLVFSFSTSMNEFWNALLEKAYAKLLGCYEALDGLTITDIIVDFTGTLAETVDMQKGRYTELVEE
  KYKLFGELYKTFTKGGLICCSIESPNQEEQEVETDWGLLKGHTYTMTDIRKIRLGERLVEVFSAEKVYMV
  RLRNPLGRQEWSGPWSEISEEWQQLTASDRKNLGLVMSDDGEFWMSLEDFCRNFHKLNVCRNVNNPIFGR
  KELESVLGCWTVDDDPLMNRSGGCYNNRDTFLQNPQYIFTVPEDGHKVIMSLQQKDLRTYRRMGRPDNYI
  IGFELFKVEMNRKFRLHHLYIQERAGTSTYIDTRTVFLSKYLKKGNYVLVPTMFQHGRTSEFLLRIFSEV
  PVQLRELTLDMPKMSCWNLARGYPKVVTQITVHSAEDLEKKYANETVNPYLVIKCGKEEVRSPVQKNTVH
  AIFDTQAIFYRRTTDIPIIVQVWNSRKFCDQFLGQVTLDADPSDCRDLKSLYLRKKGGPTAKVKQGHISF
  KVISSDDLTEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014289
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014289.2, NP_055104.2
• RefSeq Size: 3568 bp
• RefSeq ORF: 1926 bp
• Locus ID: 827
• Cytogenetics: Xq23
• Domains: C2, Calpain_III
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis. [provided by RefSeq, Nov 2009]'