Calpain 6 (CAPN6) (NM_014289) Human Untagged Clone

CAT#: SC115094

CAPN6 (untagged)-Human calpain 6 (CAPN6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAPN6
• Synonyms: CalpM; CANPX; CAPNX; DJ914P14.1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115094 sequence for NM_014289 edited (data generated by NextGen Sequencing)
  ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
  ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
  TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
  CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
  AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
  ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
  TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
  CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
  GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
  ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
  AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
  ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
  GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
  AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
  CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
  GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
  GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
  CGCAACGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
  ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
  TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
  TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
  ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
  ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
  TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
  GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
  ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
  ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
  TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
  GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
  CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
  GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
  GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
  CTCTAA
  
  Clone variation with respect to NM_014289.3
  1026 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014289 unedited
  ATTGGGATTTGTAATCCGACTTTACTATAGGGCGGACCGACGCATTCGGCACGAGGGCTA
  ACAGCAGCAGCGGCAACGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGGCTC
  CTGGGATAACTCAGGCATAGTTCAACACTATGGGTCCTCCTCTGAAGCTCTTCAAAAACC
  AGAAATACCAGGAACTGAAGCAGGAATGCATCAAAGACAGCAGACTTTTCTGTGATCCAA
  CATTTCTGCCTGAGAATGATTCTCTTTTCTACAACCGACTGCTTCCTGGAAAGGTGGTGT
  GGAAACGTCCCCAGGACATCTGTGATGACCCCCATCTGATTGTGGGCAACATTAGCAACC
  ACCAGCTGACCCAAGGGAGACTGGGGCACAAGCCAATGGTTTCTGCATTTTCCTGTTTGG
  CTGTTCAGGAGTCTCATTGGACAAAGACAATTCCCAACCATAAGGAACAGGAATGGGACC
  CTCAAAAAACAGAAAAATACGCTGGGATATTTCACTTTCGTTTCTGGCATTTTGGAGAAT
  GGACTGAAGTGGTGATTGATGACTTGTTGCCCACCATTAACGGAGATCTGGTCTTCTCTT
  TCTCCACTTCCATGAATGAGTTTTGGAATGCTCTGCTGNGAAAAGCTTATGCAAAGCTGC
  TAGGCTGTTATGAGGCCCTGGATGGTTTGACCATCACTGATATTATTGTGGACTTCACGG
  GCACATTGGCTGANACTGNTGACATGCAGAAAGGAGATACACTGAGCTTGTTGAGGAGAA
  AGTACAGCTATTTCGAGAACTGTACANACATTTACCCAAGGTGGTCTGATCTGCNTGTCC
  ATTGAGTCTCCAATCAGGGAGGAGCAGAAGTGAAACTGATGGGGGTCTGCTGAAGGCCAT
  ACCTATCCATGACTGATATCGCAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014289 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTACTGAATATGAACTTTATTGT
  GATTTGGTTTATGTAAGGCAGTGTAGTGAAGGCACTGCAGAAGTTAAACAGACTGGAAAA
  CATGGTAAAAAATGAGCGAGCTAAAAATGAATGCTTGCTGGTCTAGGTAGGCTAAGTACT
  ACAACTATGTTTCTTTTTCATCATTGCAGAAGCGTTGAAATATTGTTGAAGGTGGGGGTT
  TGGGGTGGAATTAGGGAAGGCTTCATGGAGGAGGTGGCATTTATACTGGGCCTTGAAGGA
  GGGGCAGAAAGTTGATAGGTTAAGATGGAGGGAAGTACATTTCATAATGACTGACTCTAG
  AGAAAATTCTGTAGAAGTACTGAAAAATGAGAGGGCAAACAAACACCCCCAGCGAGTGAA
  GCATCCCAACTGAGAGAAAAGTCTGAGGAGTCTGGGCTGGGACCAAAGTCTCAAGTCCCC
  AGATGGGGTCTTCAGTACAAATGATATTAGGAGGCACAGGACAATGGGCTTGTTTGTAAA
  CCTAGCAGCAGCGAGAGGTTTCACATTGCAGTCAGGGGCCCCAAACTGGCTGTAATATGT
  CTGGATGAATTCTCTCATCTTTTAGGTGTGTATGGGACTTACTTATCTTTCCTCTTGCCC
  ACTGACTGCCTGAATGGTAGTTCGTGCGCGCCCATGTGCGCGTGTGTGAATGTANGTGTG
  TGTGGATGGGTGGGGGACCCANGCTTTGTCTCCCACTGCAGCTTCTTCCACTTTTCCGTC
  TTGTGGCACACAGAAGGCTTCCATTGAGCTGAAGCTAGGCCCCTCTCACTGGTCTAGACT
  TCATTGAATTCACCTCTTGCATAAGCCCCAGGAAGCTCTCTCACATGAAGGGTAAAATTA
  CTGTCGGGGGCGACTTGTGTAAGGGCAGACTACCCTTCCCGAGTGATAAGGCGCTAAGGT
  GGGCGCCCCTCCCACACCACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014289
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014289.2, NP_055104.2
• RefSeq Size: 3568 bp
• RefSeq ORF: 1926 bp
• Locus ID: 827
• Cytogenetics: Xq23
• Domains: C2, Calpain_III
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis. [provided by RefSeq, Nov 2009]'