SEC22L1 (SEC22B) (NM_004892) Human Tagged ORF Clone

CAT#: RG200569

SEC22B (GFP-tagged) - Human SEC22 vesicle trafficking protein homolog B (S. cerevisiae) (gene/pseudogene) (SEC22B)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SEC22B
• Synonyms: ERS-24; SEC22L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200569 representing NM_004892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTGCTAACAATGATCGCCCGAGTGGCGGACGGGCTCCCGCTGGCCGCCTCGATGCAGGAGGACG
  AACAGTCTGGCCGGGACCTTCAACAGTATCAGAGTCAGGCTAAGCAACTCTTTCGAAAGTTGAATGAACA
  GTCCCCTACCAGATGTACCTTGGAAGCAGGAGCCATGACTTTTCACTACATTATTGAGCAGGGGGTGTGT
  TATTTGGTTTTATGTGAAGCTGCCTTCCCTAAGAAGTTGGCTTTTGCCTACCTAGAAGATTTGCACTCAG
  AATTTGATGAACAGCATGGAAAGAAGGTGCCCACTGTGTCCCGACCCTATTCCTTTATTGAATTTGATAC
  TTTCATTCAGAAAACCAAGAAGCTCTACATTGACAGTCGTGCTCGAAGAAATCTAGGCTCCATCAACACT
  GAATTGCAAGATGTGCAGAGGATCATGGTGGCCAATATTGAAGAAGTGTTACAACGAGGAGAAGCACTCT
  CAGCATTGGATTCAAAGGCTAACAATTTGTCCAGTCTGTCCAAGAAATACCGCCAGGATGCGAAGTACTT
  GAACATGCGTTCCACTTATGCCAAACTTGCAGCAGTAGCTGTATTTTTCATCATGTTAATAGTGTATGTC
  CGATTCTGGTGGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200569 representing NM_004892
  Red=Cloning site Green=Tags(s)
  
  MVLLTMIARVADGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQSPTRCTLEAGAMTFHYIIEQGVC
  YLVLCEAAFPKKLAFAYLEDLHSEFDEQHGKKVPTVSRPYSFIEFDTFIQKTKKLYIDSRARRNLGSINT
  ELQDVQRIMVANIEEVLQRGEALSALDSKANNLSSLSKKYRQDAKYLNMRSTYAKLAAVAVFFIMLIVYV
  RFWWL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004892
• ORF Size: 645 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004892.4, NP_004883.2
• RefSeq Size: 1821
• RefSeq ORF: 648
• Locus ID: 9554
• Domains: synaptobrevin
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins. [provided by RefSeq, Sep 2009]