Fibrillarin (FBL) (NM_001436) Human Tagged ORF Clone
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | FBL |
Synonyms | FIB; FLRN; Nop1; RNU3IP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200732 representing NM_001436
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCAGGATTCAGTCCCCGTGGGGGTGGCTTTGGCGGCCGAGGGGGCTTTGGTGACCGTGGTGGTC GTGGAGGCCGAGGGGGCTTTGGCGGGGGCCGAGGTCGAGGCGGAGGCTTTAGAGGTCGTGGACGAGGAGG AGGTGGAGGCGGCGGCGGCGGTGGAGGAGGAGGAAGAGGTGGTGGAGGCTTCCATTCTGGTGGCAACCGG GGTCGTGGTCGGGGAGGAAAAAGAGGAAACCAGTCGGGGAAGAATGTGATGGTGGAGCCGCATCGGCATG AGGGTGTCTTCATTTGTCGAGGAAAGGAAGATGCACTGGTCACCAAGAACCTGGTCCCTGGGGAATCAGT TTATGGAGAGAAGAGAGTCTCGATTTCGGAAGGAGATGACAAAATTGAGTACCGAGCCTGGAACCCCTTC CGCTCCAAGCTAGCAGCAGCAATCCTGGGTGGTGTGGACCAGATCCACATCAAACCGGGGGCTAAGGTTC TCTACCTCGGGGCTGCCTCGGGCACCACGGTCTCCCATGTCTCTGACATCGTTGGTCCGGATGGTCTAGT CTATGCAGTCGAGTTCTCCCACCGCTCTGGCCGTGACCTCATTAACTTGGCCAAGAAGAGGACCAACATC ATTCCTGTGATCGAGGATGCTCGACACCCACACAAATACCGCATGCTCATCGCAATGGTGGATGTGATCT TTGCTGATGTGGCCCAGCCAGACCAGACCCGGATTGTGGCCCTGAATGCCCACACCTTCCTGCGTAATGG AGGACACTTTGTGATTTCCATTAAGGCCAACTGCATTGACTCCACAGCCTCAGCCGAGGCCGTGTTTGCC TCCGAAGTGAAAAAGATGCAACAGGAGAACATGAAGCCGCAGGAGCAGTTGACCCTTGAGCCATATGAAA GAGACCATGCCGTGGTCGTGGGAGTGTACAGGCCACCCCCCAAGGTGAAGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200732 representing NM_001436
Red=Cloning site Green=Tags(s) MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNR GRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPF RSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNI IPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFA SEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001436 |
ORF Size | 963 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001436.2, NP_001427.2 |
RefSeq Size | 1135 bp |
RefSeq ORF | 966 bp |
Locus ID | 2091 |
Cytogenetics | 19q13.2 |
Domains | Fibrillarin |
Protein Families | Stem cell - Pluripotency |
Gene Summary | 'This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319339 | FBL (untagged)-Human fibrillarin (FBL) |
USD 310.00 |
|
RC200732 | FBL (Myc-DDK-tagged)-Human fibrillarin (FBL) |
USD 98.00 |
|
RC200732L1 | Lenti ORF clone of Human fibrillarin (FBL), Myc-DDK-tagged |
USD 768.00 |
|
RC200732L2 | Lenti ORF clone of Human fibrillarin (FBL), mGFP tagged |
USD 620.00 |
|
RC200732L3 | Lenti ORF clone of Human fibrillarin (FBL), Myc-DDK-tagged |
USD 620.00 |
|
RC200732L4 | Lenti ORF clone of Human fibrillarin (FBL), mGFP tagged |
USD 620.00 |
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