Fibrillarin (FBL) (NM_001436) Human Untagged Clone

CAT#: SC319339

FBL (untagged)-Human fibrillarin (FBL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBL
• Synonyms: FIB; FLRN; Nop1; RNU3IP1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001436.2 GCGAAAGCCCCGGACTCGTGGAGTTGTGAACGCCGCGGACTCCGGAGCCGCACAAACCAG
  GGCTCGCCATGAAGCCAGGATTCAGTCCCCGTGGGGGTGGCTTTGGCGGCCGAGGGGGCT
  TTGGTGACCGTGGTGGTCGTGGAGGCCGAGGGGGCTTTGGCGGGGGCCGAGGTCGAGGCG
  GAGGCTTTAGAGGTCGTGGACGAGGAGGAGGTGGAGGCGGCGGCGGCGGTGGAGGAGGAG
  GAAGAGGTGGTGGAGGCTTCCATTCTGGTGGCAACCGGGGTCGTGGTCGGGGAGGAAAAA
  GAGGAAACCAGTCGGGGAAGAATGTGATGGTGGAGCCGCATCGGCATGAGGGTGTCTTCA
  TTTGTCGAGGAAAGGAAGATGCACTGGTCACCAAGAACCTGGTCCCTGGGGAATCAGTTT
  ATGGAGAGAAGAGAGTCTCGATTTCGGAAGGAGATGACAAAATTGAGTACCGAGCCTGGA
  ACCCCTTCCGCTCCAAGCTAGCAGCAGCAATCCTGGGTGGTGTGGACCAGATCCACATCA
  AACCGGGGGCTAAGGTTCTCTACCTCGGGGCTGCCTCGGGCACCACGGTCTCCCATGTCT
  CTGACATCGTTGGTCCGGATGGTCTAGTCTATGCAGTCGAGTTCTCCCACCGCTCTGGCC
  GTGACCTCATTAACTTGGCCAAGAAGAGGACCAACATCATTCCTGTGATCGAGGATGCTC
  GACACCCACACAAATACCGCATGCTCATCGCAATGGTGGATGTGATCTTTGCTGATGTGG
  CCCAGCCAGACCAGACCCGGATTGTGGCCCTGAATGCCCACACCTTCCTGCGTAATGGAG
  GACACTTTGTGATTTCCATTAAGGCCAACTGCATTGACTCCACAGCCTCAGCCGAGGCCG
  TGTTTGCCTCCGAAGTGAAAAAGATGCAACAGGAGAACATGAAGCCGCAGGAGCAGTTGA
  CCCTTGAGCCATATGAAAGAGACCATGCCGTGGTCGTGGGAGTGTACAGGCCACCCCCCA
  AGGTGAAGAACTGAAGTTCAGCGCTGTCAGGATTGCGAGAGATGTGTGTTGATACTGTTG
  CACGTGTGTTTTTCTATTAAAAGACTCATCCGTCAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001436
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001436.2, NP_001427.2
• RefSeq Size: 1135 bp
• RefSeq ORF: 966 bp
• Locus ID: 2091
• Cytogenetics: 19q13.2
• Domains: Fibrillarin
• Protein Families: Stem cell - Pluripotency
• Gene Summary: 'This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]'