MDS028 (ITFG2) (NM_018463) Human Tagged ORF Clone

CAT#: RG200818

ITFG2 (GFP-tagged) - Human integrin alpha FG-GAP repeat containing 2 (ITFG2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ITFG2
• Synonyms: FGGAP1; MDS028
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200818 representing NM_018463
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTCAGTTAGCTACGTGCAGCGCGTGGCGCTGGAGTTCAGCGGGAGCCTCTTCCCGCACGCAATCT
  GCCTCGGAGACGTTGATAACGATACGTTAAATGAACTGGTGGTGGGAGACACCAGCGGGAAGGTGTCTGT
  GTATAAAAATGATGACAGTCGGCCATGGCTCACCTGTTCCTGCCAGGGAATGCTGACTTGCGTTGGGGTT
  GGAGACGTGTGTAATAAAGGAAAGAACCTGTTGGTGGCAGTGAGTGCTGAAGGCTGGTTTCATTTGTTTG
  ACCTGACACCTGCCAAGGTGTTGGATGCTTCTGGGCACCACGAGACACTAATCGGAGAGGAGCAGCGTCC
  AGTCTTCAAGCAGCACATCCCTGCCAACACCAAGGTCATGCTGATCAGCGACATCGATGGAGATGGGTGT
  CGTGAGCTGGTGGTGGGCTACACAGACCGTGTGGTGCGAGCTTTCCGCTGGGAGGAGCTAGGTGAGGGTC
  CTGAACATCTGACAGGGCAGCTGGTGTCCCTCAAGAAATGGATGCTGGAGGGTCAGGTGGACAGCCTCTC
  AGTGACTCTGGGGCCACTGGGTCTTCCTGAACTGATGGTGTCTCAGCCAGGTTGTGCGTATGCAATTCTA
  CTGTGTACCTGGAAAAAGGACACTGGGTCCCCTCCTGCCTCTGAAGGGCCCACGGATGGTAGTAGGGAGA
  CCCCAGCTGCCCGAGACGTGGTGCTGCACCAGACATCTGGCCGTATCCACAACAAGAATGTCTCCACTCA
  CCTAATTGGCAACATCAAACAAGGCCACGGCACTGAGAGTAGTGGCTCTGGCCTCTTTGCCCTGTGCACC
  CTGGATGGGACACTGAAGCTCATGGAAGAAATGGAAGAAGCAGACAAGCTGCTGTGGTCAGTGCAGGTGG
  ATCACCAGCTCTTTGCCCTGGAGAAACTGGATGTCACCGGCAACGGGCATGAGGAGGTAGTTGCATGCGC
  CTGGGATGGACAGACATATATCATTGATCACAACCGCACCGTCGTCCGCTTCCAAGTGGATGAAAATATC
  CGTGCCTTCTGTGCAGGCCTGTACGCCTGCAAAGAGGGCCGCAACAGCCCCTGCCTCGTATATGTCACTT
  TCAACCAGAAGATCTATGTGTACTGGGAGGTGCAGCTGGAGCGGATGGAGTCTACCAATCTGGTGAAACT
  GCTGGAGACCAAGCCGGAGTACCACAGCCTGCTGCAGGAGCTGGGCGTGGATCCTGACGACCTCCCTGTG
  ACTCGTGCCCTGCTTCACCAAACGCTCTACCATCCAGACCAGCCACCACAGTGTGCTCCCTCAAGCCTCC
  AGGATCCCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200818 representing NM_018463
  Red=Cloning site Green=Tags(s)
  
  MRSVSYVQRVALEFSGSLFPHAICLGDVDNDTLNELVVGDTSGKVSVYKNDDSRPWLTCSCQGMLTCVGV
  GDVCNKGKNLLVAVSAEGWFHLFDLTPAKVLDASGHHETLIGEEQRPVFKQHIPANTKVMLISDIDGDGC
  RELVVGYTDRVVRAFRWEELGEGPEHLTGQLVSLKKWMLEGQVDSLSVTLGPLGLPELMVSQPGCAYAIL
  LCTWKKDTGSPPASEGPTDGSRETPAARDVVLHQTSGRIHNKNVSTHLIGNIKQGHGTESSGSGLFALCT
  LDGTLKLMEEMEEADKLLWSVQVDHQLFALEKLDVTGNGHEEVVACAWDGQTYIIDHNRTVVRFQVDENI
  RAFCAGLYACKEGRNSPCLVYVTFNQKIYVYWEVQLERMESTNLVKLLETKPEYHSLLQELGVDPDDLPV
  TRALLHQTLYHPDQPPQCAPSSLQDPT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018463
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018463.2, NP_060933.2
• RefSeq Size: 2155
• RefSeq ORF: 1344
• Locus ID: 55846
• Domains: FG-GAP
• Protein Families: Druggable Genome
• Gene Summary: As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose. [UniProtKB/Swiss-Prot Function]