XPNPEP3 (NM_022098) Human Tagged ORF Clone

CAT#: RG200888

XPNPEP3 (GFP-tagged) - Human X-prolyl aminopeptidase (aminopeptidase P) 3, putative (XPNPEP3), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: XPNPEP3
• Synonyms: APP3; ICP55; NPHPL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200888 representing NM_022098
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTGGCTGCTCTCAGCCCCCAAGCTGGTTCCCGCTGTAGCAAACGTCCGCGGCCTCTCAGGATGTA
  TGTTGTGTTCACAGCGAAGGTACTCCCTTCAGCCTGTCCCAGAAAGGAGGATTCCAAACCGATACTTAGG
  CCAGCCCAGCCCCTTTACACACCCACACCTCCTCAGACCAGGGGAGGTAACTCCAGGACTATCTCAGGTG
  GAATATGCACTTCGCAGACACAAACTAATGTCTCTGATCCAGAAGGAAGCTCAAGGGCAGAGTGGGACAG
  ACCAGACAGTGGTTGTGCTCTCCAACCCTACATACTACATGAGCAACGATATTCCCTATACTTTCCACCA
  AGACAACAATTTCCTGTACCTATGTGGATTCCAAGAGCCTGATAGCATTCTTGTCCTTCAGAGCCTCCCT
  GGCAAACAATTACCATCACACAAAGCCATACTTTTTGTGCCTCGGCGAGATCCCAGTCGAGAACTTTGGG
  ATGGTCCGCGATCTGGCACTGATGGAGCAATAGCTCTAACTGGAGTAGACGAAGCCTATACGCTAGAAGA
  ATTTCAACATCTTCTACCAAAAATGAAAGCTGAGACGAACATGGTTTGGTATGACTGGATGAGGCCCTCA
  CATGCACAGCTTCACTCTGACTATATGCAGCCCCTGACTGAGGCCAAAGCCAAGAGCAAGAACAAGGTTC
  GGGGTGTTCAGCAGCTGATACAGCGCCTCCGGCTGATCAAGTCTCCTGCAGAAATTGAACGAATGCAGAT
  TGCTGGGAAGCTGACATCACAGGCTTTCATAGAAACCATGTTCACCAGTAAAGCCCCTGTGGAAGAAGCC
  TTTCTTTATGCTAAGTTTGAATTTGAATGCCGGGCTCGTGGCGCAGACATTTTAGCCTATCCACCTGTGG
  TGGCTGGTGGTAATCGGTCAAACACTTTGCACTATGTGAAAAATAATCAACTCATCAAGGATGGGGAAAT
  GGTGCTTCTGGATGGAGGTTGTGAGTCTTCCTGCTATGTGAGTGACATCACACGTACGTGGCCAGTCAAT
  GGCAGGTTCACCGCACCTCAGGCAGAACTCTATGAAGCCGTTCTAGAGATCCAAAGAGATTGTTTGGCCC
  TCTGCTTCCCTGGGACAAGCTTGGAGAACATCTACAGCATGATGCTGACCCTGATAGGACAGAAGCTTAA
  AGACTTGGGGATCATGAAGAACATTAAGGAAAATAATGCCTTCAAGGCTGCTCGAAAATACTGTCCTCAT
  CATGTTGGCCACTACCTCGGGATGGATGTCCATGACACTCCAGACATGCCCCGTTCCCTCCCTCTGCAGC
  CTGGGATGGTAATCACAATTGAGCCCGGCATTTATATTCCAGAGGATGACAAAGATGCCCCAGAGAAGTT
  TCGGGGTCTTGGTGTACGAATTGAGGATGATGTAGTGGTGACTCAGGACTCACCTTTCATCCTTTCTGCA
  GACTGTCCCAAAGAGATGAATGACATTGAACAGATATGCAGCCAGGCTTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200888 representing NM_022098
  Red=Cloning site Green=Tags(s)
  
  MPWLLSAPKLVPAVANVRGLSGCMLCSQRRYSLQPVPERRIPNRYLGQPSPFTHPHLLRPGEVTPGLSQV
  EYALRRHKLMSLIQKEAQGQSGTDQTVVVLSNPTYYMSNDIPYTFHQDNNFLYLCGFQEPDSILVLQSLP
  GKQLPSHKAILFVPRRDPSRELWDGPRSGTDGAIALTGVDEAYTLEEFQHLLPKMKAETNMVWYDWMRPS
  HAQLHSDYMQPLTEAKAKSKNKVRGVQQLIQRLRLIKSPAEIERMQIAGKLTSQAFIETMFTSKAPVEEA
  FLYAKFEFECRARGADILAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVN
  GRFTAPQAELYEAVLEIQRDCLALCFPGTSLENIYSMMLTLIGQKLKDLGIMKNIKENNAFKAARKYCPH
  HVGHYLGMDVHDTPDMPRSLPLQPGMVITIEPGIYIPEDDKDAPEKFRGLGVRIEDDVVVTQDSPFILSA
  DCPKEMNDIEQICSQAS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022098
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022098.2, NP_071381.1
• RefSeq Size: 3060
• RefSeq ORF: 1524
• Locus ID: 63929
• Domains: Peptidase_M24, AMP_N
• Protein Families: Druggable Genome, Protease
• Gene Summary: The protein encoded by this gene belongs to the family of X-pro-aminopeptidases that utilize a metal cofactor, and remove the N-terminal amino acid from peptides with a proline residue in the penultimate position. This protein has been shown to localize to the mitochondria of renal cells, and have a role in ciliary function. Mutations in this gene are associated with nephronophthisis-like nephropathy-1. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene, however, expression of some of these isoforms in vivo is not known. [provided by RefSeq, Mar 2011]