OTUD5 (NM_017602) Human Tagged ORF Clone

CAT#: RG200893

OTUD5 (GFP-tagged) - Human OTU domain containing 5 (OTUD5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OTUD5
• Synonyms: DUBA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200893 representing NM_017602
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGGCGCGCCC
  
  ATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGACCCCGCCAACGAGCCGCCGCCGCCCG
  GGCCGATGCCCCCGGCGCCGCGGCGCGGCGGAGGTGTGGGCGTGGGCGGCGGCGGCACGGGCGTGGGCGG
  CGGCGATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCCACCGCCTCAAGGCCCG
  CTACCAGGACCGCCGGGCGCTCTTCATCGCTGGGCGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCC
  GGCCACAACAGGCTTCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGACGCGCT
  GGGCGCAGCGGCGGCGGGTGTGGGTGCCGCGGGCGTGGTGGTGGGTGTGGGTGGTGCCGTAGGCGTGGGC
  GGCTGCTGCTCCGGGCCTGGGCACAGCAAGCGGCGACGTCAAGCTCCCGGGGTTGGCGCGGTTGGCGGGG
  GCAGTCCCGAGCGTGAGGAGGTCGGCGCAGGCTACAACAGTGAGGACGAGTATGAGGCGGCTGCAGCACG
  CATCGAGGCTATGGACCCTGCCACTGTCGAGCAGCAGGAGCATTGGTTTGAAAAGGCCCTACGAGACAAG
  AAGGGCTTCATCATCAAGCAGATGAAGGAGGATGGCGCCTGTCTCTTCCGGGCTGTAGCTGACCAGGTGT
  ATGGAGACCAGGACATGCATGAGGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTA
  CTTCTCCAACTATGTCACAGAGGACTTTACCACCTACATTAACAGGAAGCGGAAAAACAATTGCCATGGC
  AACCACATTGAGATGCAGGCCATGGCAGAGATGTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAG
  GTACTTCTGCAGTGGAACCCATCAACACATTCCATGGGATACATCAAAACGAGGACGAACCCATTCGTGT
  TAGCTACCATCGGAATATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACCATTGGTGTGGGGCTG
  GGCCTGCCATCATTCAAACCAGGGTTTGCAGAGCAGTCTCTGATGAAGAATGCCATAAAAACATCGGAGG
  AGTCATGGATTGAACAGCAGATGCTAGAAGACAAGAAACGGGCCACAGACTGGGAGGCCACAAATGAAGC
  CATCGAGGAGCAGGTGGCTCGGGAATCCTACCTGCAGTGGTTGCGGGATCAGGAGAAACAGGCTCGCCAG
  GTCCGAGGCCCCAGCCAGCCCCGGAAAGCCAGCGCCACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTG
  GCCTGGAGGAGTGGACTAGCCGGTCCCCGCGGCAGCGGAGTTCAGCCTCGTCACCTGAGCACCCTGAGCT
  GCATGCTGAATTGGGCATGAAGCCCCCTTCCCCAGGCACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCC
  TGTGCGCCAGGTACAAGCAGTCAGTTCTCGGCAGGGGCCGACCGGGCAACTTCCCCCCTTGTGTCCCTCT
  ACCCTGCTTTGGAGTGCCGGGCCCTCATTCAGCAGATGTCCCCCTCTGCCTTTGGTCTGAATGACTGGGA
  TGATGATGAGATCCTAGCTTCGGTGCTGGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAAC
  AAAGTGCACAGAGACCCGCCCCCAGACAAGAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200893 representing NM_017602
  Red=Cloning site Green=Tags(s)
  
  MTILPKKKPPPPDADPANEPPPPGPMPPAPRRGGGVGVGGGGTGVGGGDRDRDSGVVGARPRASPPPQGP
  LPGPPGALHRWALAVPPGAVAGPRPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVG
  GCCSGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDK
  KGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHG
  NHIEMQAMAEMYNRPVEVYQYSTGTSAVEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGL
  GLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQ
  VRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSP
  CAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKN
  KVHRDPPPDKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017602
• ORF Size: 1713 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017602.2, NP_060072.1
• RefSeq Size: 2759
• RefSeq ORF: 1716
• Locus ID: 55593
• Protein Families: Protease
• Protein Pathways: RIG-I-like receptor signaling pathway
• Gene Summary: This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]