GSTM3 (NM_000849) Human Tagged ORF Clone
CAT#: RG201013
- TrueORF®
GSTM3 (GFP-tagged) - Human glutathione S-transferase mu 3 (brain) (GSTM3), transcript variant 1
"NM_000849" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | GSTM3 |
Synonyms | GST5; GSTB; GSTM3-3; GTM3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201013 representing NM_000849
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTGCGAGTCGTCTATGGTTCTCGGGTACTGGGATATTCGTGGGCTGGCGCACGCCATCCGCCTGC TCCTGGAGTTCACGGATACCTCTTATGAGGAGAAACGGTACACGTGCGGGGAAGCTCCTGACTATGATCG AAGCCAATGGCTGGATGTGAAATTCAAGCTAGACCTGGACTTTCCTAATCTGCCCTACCTCCTGGATGGG AAGAACAAGATCACCCAGAGCAATGCCATCTTGCGCTACATCGCTCGCAAGCACAACATGTGTGGTGAGA CTGAAGAAGAAAAGATTCGAGTGGACATCATAGAGAACCAAGTAATGGATTTCCGCACACAACTGATAAG GCTCTGTTACAGCTCTGACCACGAAAAACTGAAGCCTCAGTACTTGGAAGAGCTACCTGGACAACTGAAA CAATTCTCCATGTTTCTGGGGAAATTCTCATGGTTTGCCGGGGAAAAGCTCACCTTTGTGGATTTTCTCA CCTATGATATCTTGGATCAGAACCGTATATTTGACCCCAAGTGCCTGGATGAGTTCCCAAACCTGAAGGC TTTCATGTGCCGTTTTGAGGCTTTGGAGAAAATCGCTGCCTACTTACAGTCTGATCAGTTCTGCAAGATG CCCATCAACAACAAGATGGCCCAGTGGGGCAACAAGCCTGTATGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201013 representing NM_000849
Red=Cloning site Green=Tags(s) MSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDG KNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLK QFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKM PINNKMAQWGNKPVC TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000849 |
ORF Size | 675 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000849.3, NP_000840.2 |
RefSeq Size | 3948 bp |
RefSeq ORF | 678 bp |
Locus ID | 2947 |
Cytogenetics | 1p13.3 |
Domains | GST_N, GST_C |
Protein Pathways | Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450 |
Gene Summary | 'Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319401 | GSTM3 (untagged)-Human glutathione S-transferase mu 3 (brain) (GSTM3), transcript variant 1 |
USD 310.00 |
|
RC201013 | GSTM3 (Myc-DDK-tagged)-Human glutathione S-transferase mu 3 (brain) (GSTM3), transcript variant 1 |
USD 98.00 |
|
RC201013L3 | Lenti ORF clone of Human glutathione S-transferase mu 3 (brain) (GSTM3), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC201013L4 | Lenti ORF clone of Human glutathione S-transferase mu 3 (brain) (GSTM3), transcript variant 1, mGFP tagged |
USD 620.00 |
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