AP2B1 (NM_001030006) Human Tagged ORF Clone
CAT#: RG201129
- TrueORF®
AP2B1 (GFP-tagged) - Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1
"NM_001030006" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | AP2B1 |
Synonyms | ADTB2; AP2-BETA; AP105B; CLAPB1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201129 representing NM_001030006
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACTCCAAGTATTTCACAACCAATAAAAAAGGAGAAATATTTGAACTAAAAGCTGAACTCAACA ATGAAAAGAAAGAAAAGAGAAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGATGT TAGTTCTCTCTTTCCAGACGTAGTGAACTGTATGCAGACTGACAATCTGGAACTAAAGAAGCTTGTGTAT CTCTACTTGATGAACTACGCCAAGAGTCAGCCAGACATGGCCATCATGGCTGTAAACAGCTTTGTGAAGG ACTGTGAAGATCCTAATCCTTTGATTCGAGCCTTGGCAGTCAGAACCATGGGGTGCATCCGGGTAGACAA AATTACAGAATATCTCTGTGAGCCGCTCCGCAAGTGCTTGAAGGATGAGGATCCCTATGTTCGGAAAACA GCAGCAGTCTGCGTGGCAAAACTCCATGATATCAATGCCCAAATGGTGGAAGATCAGGGATTTCTGGATT CTCTACGGGATCTCATAGCAGATTCAAATCCAATGGTGGTGGCTAATGCCGTAGCGGCATTATCTGAAAT CAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCACAGAACATTAATAAGCTGCTGACAGCC CTGAATGAATGCACTGAATGGGGCCAGATTTTCATCCTGGACTGCCTGTCTAATTACAACCCTAAAGATG ATCGGGAGGCTCAGAGCATCTGTGAGCGGGTAACTCCCCGGCTATCCCATGCCAACTCAGCAGTGGTGCT TTCAGCGGTAAAAGTCCTAATGAAGTTTCTAGAATTGTTACCTAAGGATTCTGACTACTACAATATGCTG CTGAAGAAGTTAGCCCCTCCACTTGTCACTTTGCTGTCTGGGGAGCCAGAAGTGCAGTATGTCGCCCTGA GGAACATCAACTTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAAATCAAAGTCTTCTTTGTGAA GTACAATGATCCCATCTATGTTAAACTAGAGAAGTTGGACATCATGATTCGTTTGGCATCTCAAGCCAAC ATTGCTCAGGTTCTGGCAGAACTGAAAGAATATGCTACAGAGGTGGATGTTGACTTTGTTCGAAAAGCTG TGCGGGCCATTGGACGGTGTGCCATCAAGGTGGAGCAATCTGCAGAGCGCTGTGTAAGCACATTGCTTGA TCTAATCCAGACCAAAGTGAATTATGTGGTCCAAGAAGCAATTGTTGTCATCAGGGACATCTTCCGCAAA TACCCCAACAAGTATGAAAGTATCATCGCCACTCTGTGTGAGAACTTAGACTCGCTGGATGAGCCAGATG CTCGAGCAGCTATGATTTGGATTGTGGGAGAATATGCTGAAAGAATTGACAATGCAGATGAGTTACTAGA AAGCTTCCTGGAGGGTTTTCACGATGAAAGCACCCAGGTGCAGCTCACTCTGCTTACTGCCATAGTGAAG CTGTTTCTCAAGAAACCATCAGAAACACAGGAGCTAGTCCAGCAGGTCTTGAGTTTGGCAACACAGGATT CTGATAATCCTGACCTTCGAGACCGGGGCTATATTTATTGGCGCCTTCTCTCAACTGACCCTGTTACAGC TAAAGAAGTAGTCTTGTCTGAGAAGCCACTGATCTCTGAGGAGACGGACCTTATTGAGCCAACTCTGCTG GATGAGCTAATCTGCCACATTGGTTCTTTGGCCTCTGTGTATCATAAGCCTCCCAATGCTTTTGTGGAAG GAAGTCATGGAATTCATCGTAAACACTTGCCAATTCATCATGGGAGCACTGATGCAGGTGACAGCCCTGT TGGCACTACCACTGCAACGAACCTGGAACAGCCTCAGGTTATCCCCTCTCAAGGTGATCTTCTAGGGGAT CTTTTAAACCTTGACCTCGGTCCCCCAGTCAATGTGCCACAGGTGTCCTCCATGCAGATGGGAGCAGTGG ATCTCCTAGGAGGAGGACTAGATAGTCTGCTTGGCAGTGACCTTGGCGGGGGCATTGGAGGAAGTCCGGC AGTGGGACAATCCTTCATCCCATCATCGGTCCCTGCAACCTTTGCTCCTTCACCTACACCTGCTGTGGTC AGCAGTGGACTGAATGACCTGTTTGAACTCTCCACAGGGATAGGCATGGCACCTGGTGGATATGTGGCTC CTAAGGCTGTCTGGCTACCTGCAGTAAAGGCTAAAGGCTTGGAGATTTCCGGAACATTTACTCACCGCCA AGGGCACATCTATATGGAAATGAACTTCACCAATAAAGCTCTGCAGCACATGACAGATTTTGCAATCCAG TTTAACAAAAATAGCTTTGGTGTCATCCCCAGCACTCCTCTGGCCATCCATACACCACTGATGCCAAACC AGAGCATTGATGTCTCCCTGCCTCTCAATACCTTGGGCCCAGTCATGAAGATGGAACCTCTGAATAACCT CCAGGTGGCTGTGAAAAACAATATCGATGTCTTCTACTTCAGCTGCCTCATCCCACTCAATGTGCTTTTT GTAGAAGATGGCAAAATGGAGCGCCAGGTCTTCCTTGCAACATGGAAGGATATTCCCAATGAAAATGAAC TTCAGTTTCAGATTAAGGAATGTCATTTAAATGCTGACACTGTTTCCAGCAAGTTGCAAAACAACAATGT TTATACTATTGCCAAGAGGAATGTGGAAGGGCAGGACATGCTGTACCAATCCCTGAAGCTCACTAATGGC ATTTGGATTTTGGCCGAACTACGTATCCAGCCAGGAAACCCCAATTACACGCTGTCACTGAAGTGTAGAG CTCCTGAAGTCTCTCAATACATCTATCAGGTCTACGACAGCATTTTGAAAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201129 representing NM_001030006
Red=Cloning site Green=Tags(s) MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVY LYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT AAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTA LNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNML LKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQAN IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRK YPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVK LFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLL DELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTATNLEQPQVIPSQGDLLGD LLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLLGSDLGGGIGGSPAVGQSFIPSSVPATFAPSPTPAVV SSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQ FNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLF VEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNG IWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001030006 |
ORF Size | 2853 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001030006.1, NP_001025177.1 |
RefSeq Size | 5772 bp |
RefSeq ORF | 2856 bp |
Locus ID | 163 |
Cytogenetics | 17q12 |
Protein Families | Druggable Genome |
Protein Pathways | Endocytosis, Huntington's disease |
Gene Summary | 'The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC302488 | AP2B1 (untagged)-Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1 |
USD 760.00 |
|
RC201129 | AP2B1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1 |
USD 420.00 |
|
RC201129L1 | Lenti ORF clone of Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC201129L2 | Lenti ORF clone of Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC201129L3 | Lenti ORF clone of Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC201129L4 | Lenti ORF clone of Human adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1, mGFP tagged |
USD 620.00 |
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