EXOSC7 (NM_015004) Human Tagged ORF Clone

CAT#: RG201419

EXOSC7 (GFP-tagged) - Human exosome component 7 (EXOSC7), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EXOSC7
• Synonyms: EAP1; hRrp42p; p8; RRP42; Rrp42p
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201419 representing NM_015004
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCGTGACGCTGAGCGAGGCGGAGAAGGTGTACATCGTGCATGGCGTCCAGGAAGACCTCCGTG
  TGGATGGCCGTGGCTGTGAGGACTACCGATGTGTCGAAGTGGAAACTGATGTGGTGTCCAACACTAGTGG
  GTCCGCCAGGGTCAAGCTGGGTCACACAGACATCTTGGTGGGAGTGAAAGCAGAAATGGGGACGCCGAAG
  CTGGAGAAACCAAATGAAGGCTACTTGGAGTTCTTTGTTGACTGTTCAGCCAGTGCTACCCCTGAATTTG
  AAGGTAGAGGAGGTGATGACCTTGGCACCGAGATCGCTAACACCCTCTATCGGATATTTAACAATAAAAG
  CAGTGTCGACTTAAAGACCCTCTGCATTAGTCCTCGGGAGCACTGCTGGGTTCTCTATGTGGATGTGCTG
  CTTCTGGAATGTGGTGGAAATTTGTTTGATGCCATTTCCATTGCTGTAAAGGCTGCTCTCTTCAATACAA
  GGATACCAAGGGTTCGAGTTTTGGAGGATGAAGAGGGGTCGAAGGACATTGAATTGTCAGATGACCCTTA
  TGACTGCATACGACTAAGTGTGGAGAATGTCCCCTGCATTGTCACTCTGTGCAAGATTGGCTATCGGCAT
  GTGGTGGATGCTACTCTTCAGGAGGAGGCCTGCTCGCTGGCCAGCTTGCTGGTGTCGGTGACCAGCAAGG
  GAGTTGTGACGTGCATGAGGAAAGTGGGGAAGGGCAGCCTGGACCCAGAGAGCATCTTCGAGATGATGGA
  GACTGGCAAGCGTGTGGGCAAGGTACTGCATGCCTCCTTGCAGAGTGTTCTGCACAAGGAAGAAAGCCTG
  GGGCCCAAGAGACAGAAAGTTGGATTCCTGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201419 representing NM_015004
  Red=Cloning site Green=Tags(s)
  
  MASVTLSEAEKVYIVHGVQEDLRVDGRGCEDYRCVEVETDVVSNTSGSARVKLGHTDILVGVKAEMGTPK
  LEKPNEGYLEFFVDCSASATPEFEGRGGDDLGTEIANTLYRIFNNKSSVDLKTLCISPREHCWVLYVDVL
  LLECGGNLFDAISIAVKAALFNTRIPRVRVLEDEEGSKDIELSDDPYDCIRLSVENVPCIVTLCKIGYRH
  VVDATLQEEACSLASLLVSVTSKGVVTCMRKVGKGSLDPESIFEMMETGKRVGKVLHASLQSVLHKEESL
  GPKRQKVGFLG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015004
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015004.2, NP_055819.1
• RefSeq Size: 1036
• RefSeq ORF: 876
• Locus ID: 23016
• Domains: RNase_PH_C
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation
• Gene Summary: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. [UniProtKB/Swiss-Prot Function]