PGD (NM_002631) Human Tagged ORF Clone

CAT#: RG201729

PGD (GFP-tagged) - Human phosphogluconate dehydrogenase (PGD)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PGD
• Synonyms: 6PGD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201729 representing NM_002631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAAGCTGACATCGCGCTGATCGGATTGGCCGTCATGGGCCAGAACTTAATTCTGAACATGAATG
  ACCACGGCTTTGTGGTCTGTGCTTTTAATAGGACTGTCTCCAAAGTTGATGATTTCTTGGCCAATGAGGC
  AAAGGGAACCAAAGTGGTGGGTGCCCAGTCCCTGAAAGAGATGGTCTCCAAGCTGAAGAAGCCCCGGCGG
  ATCATCCTCCTGGTGAAGGCTGGGCAAGCTGTGGATGATTTCATCGAGAAATTGGTACCATTGTTGGATA
  CTGGTGACATCATCATTGACGGAGGAAATTCTGAATATAGGGACACCACAAGACGGTGCCGAGACCTCAA
  GGCCAAGGGAATTTTATTTGTGGGGAGCGGAGTCAGTGGTGGAGAGGAAGGGGCCCGGTATGGCCCATCG
  CTCATGCCAGGAGGGAACAAAGAAGCGTGGCCCCACATCAAGACCATCTTCCAAGGCATTGCTGCAAAAG
  TGGGAACTGGAGAACCCTGCTGTGACTGGGTGGGAGATGAGGGAGCAGGCCACTTCGTGAAGATGGTGCA
  CAACGGGATAGAGTATGGGGACATGCAGCTGATCTGTGAGGCATACCACCTGATGAAAGACGTGCTGGGC
  ATGGCGCAGGACGAGATGGCCCAGGCCTTTGAGGATTGGAATAAGACAGAGCTAGACTCATTCCTGATTG
  AAATCACAGCCAATATTCTCAAGTTCCAAGACACCGATGGCAAACACCTGCTGCCAAAGATCAGGGACAG
  CGCGGGGCAGAAGGGCACAGGGAAGTGGACCGCCATCTCCGCCCTGGAATACGGCGTACCCGTCACCCTC
  ATTGGAGAAGCTGTCTTTGCTCGGTGCTTATCATCTCTGAAGGATGAGAGAATTCAAGCTAGCAAAAAGC
  TGAAGGGTCCCCAGAAGTTCCAGTTTGATGGTGATAAGAAATCATTCCTGGAGGACATTCGGAAGGCACT
  CTACGCTTCCAAGATCATCTCTTACGCTCAAGGCTTTATGCTGCTAAGGCAGGCAGCCACCGAGTTTGGC
  TGGACTCTCAATTATGGTGGCATCGCCCTGATGTGGAGAGGGGGCTGCATCATTAGAAGTGTATTCCTAG
  GAAAGATAAAGGATGCATTTGATCGAAACCCGGAACTTCAGAACCTCCTACTGGACGACTTCTTTAAGTC
  AGCTGTTGAAAACTGCCAGGACTCCTGGCGGCGGGCAGTCAGCACTGGGGTCCAGGCTGGCATTCCCATG
  CCCTGTTTTACCACTGCCCTCTCCTTCTATGACGGGTACAGACATGAGATGCTTCCAGCCAGCCTCATCC
  AGGCTCAGCGGGATTACTTCGGGGCTCACACCTATGAACTCTTGGCCAAACCAGGGCAGTTTATCCACAC
  CAACTGGACAGGCCATGGTGGCACCGTGTCATCCTCGTCATACAATGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201729 representing NM_002631
  Red=Cloning site Green=Tags(s)
  
  MAQADIALIGLAVMGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQSLKEMVSKLKKPRR
  IILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARYGPS
  LMPGGNKEAWPHIKTIFQGIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLG
  MAQDEMAQAFEDWNKTELDSFLIEITANILKFQDTDGKHLLPKIRDSAGQKGTGKWTAISALEYGVPVTL
  IGEAVFARCLSSLKDERIQASKKLKGPQKFQFDGDKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFG
  WTLNYGGIALMWRGGCIIRSVFLGKIKDAFDRNPELQNLLLDDFFKSAVENCQDSWRRAVSTGVQAGIPM
  PCFTTALSFYDGYRHEMLPASLIQAQRDYFGAHTYELLAKPGQFIHTNWTGHGGTVSSSSYNA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002631
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002631.2, NP_002622.2
• RefSeq Size: 1937 bp
• RefSeq ORF: 1452 bp
• Locus ID: 5226
• Cytogenetics: 1p36.22
• Domains: 6PGD, NAD_binding_2
• Protein Pathways: Glutathione metabolism, Metabolic pathways, Pentose phosphate pathway
• Gene Summary: '6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]'