EDC3 (NM_025083) Human Tagged ORF Clone

CAT#: RG202321

EDC3 (GFP-tagged) - Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EDC3
• Synonyms: hYjeF_N2-15q23; LSM16; MRT50; YJDC; YJEFN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202321 representing NM_025083
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGGAGATAGCTTGGGTGTCTATCAGGGAA
  GAGTGTCAGCTGTGGATCAGGTCAGCCAGACCATTTCTCTCACCCGGCCTTTCCATAATGGAGTGAAGTG
  TCTTGTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGAGTTAAAAATTCTGGAGATACCAGGACCT
  GGAGACAACCAACATTTTGGAGACCTTCATCAAACAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGG
  GCATCAATCAGAATGGCACAGGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCAGAATAT
  CCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCCCGCAGCAGCAACAGTGCTCAAAGAGC
  TATGTCGACAGGCACATGGAATCCTTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAACTCCTGGTCAT
  CTAGTAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAGAATGGCCAGATGAAGAATAA
  AGATGACGAGTGCTTCGGGGATGATATTGAGGAGATCCCAGACACAGATTTTGATTTTGAAGGGAACCTG
  GCTCTTTTTGACAAGGCAGCTGTGTTTGAGGAGATTGATACCTATGAAAGGAGAAGTGGTACCCGTTCCC
  GGGGCATCCCAAATGAAAGGCCCACTCGGTACCGCCATGATGAGAACATCTTGGAGTCCGAGCCCATTGT
  CTATCGACGGATCATAGTGCCCCACAACGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTTGTCCCA
  AGTATTTCCTATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGGCTGACCCTTGAGCGGA
  GACTGGAGATGACAGGTGTGTGTGCCAGTCAGATGGCACTGACCCTCCTCGGAGGACCTAACAGGTTGAA
  TCCCAAAAATGTTCACCAGAGGCCTACAGTGGCTCTACTGTGTGGACCTCATGTGAAGGGGGCTCAGGGT
  ATCAGCTGTGGAAGGCACCTAGCCAACCATGATGTCCAGGTCATCCTTTTCCTGCCCAATTTTGTCAAGA
  TGTTGGAATCTATCACCAATGAGCTGTCGCTCTTCAGCAAGACCCAAGGCCAACAAGTGTCTAGCCTCAA
  AGATCTGCCCACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCTGAGAACGTCTTCCTGCGC
  GATCAACCCTGGTACAAGGCAGCTGTGGCCTGGGCCAACCAGAACCGGGCACCAGTACTCAGCATAGACC
  CTCCTGTGCATGAAGTCGAACAGGGCATTGATGCCAAATGGTCACTGGCACTGGGCCTGCCTCTGCCACT
  GGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGCATTCCCCAGCAGGTCTTCCAGGAGGTGGGC
  ATCAACTACCACTCGCCCTTTGGCTGCAAGTTTGTTATCCCACTGCACTCTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202321 representing NM_025083
  Red=Cloning site Green=Tags(s)
  
  MATDWLGSIVSINCGDSLGVYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTFRAGDITELKILEIPGP
  GDNQHFGDLHQTELGPSGAGCQVGINQNGTGKFVKKPASSSSAPQNIPKRTDVKSQDVAVSPQQQQCSKS
  YVDRHMESLSQSKSFRRRHNSWSSSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEGNL
  ALFDKAAVFEEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVSKEFCTDSGLVVP
  SISYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQG
  ISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDCPENVFLR
  DQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGEHAGRIYLCDIGIPQQVFQEVG
  INYHSPFGCKFVIPLHSA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025083
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025083.2, NP_079359.2
• RefSeq Size: 3755
• RefSeq ORF: 1527
• Locus ID: 80153
• Domains: YjeF_N
• Protein Pathways: RNA degradation
• Gene Summary: This gene encodes a protein that is important in mRNA degradation. The encoded protein is a component of a decapping complex that promotes efficient removal of the monomethylguanosine (m7G) cap from mRNAs, as part of the 5' to 3' mRNA decay pathway. Mutations in this gene have been identified in human patients with an autosomal recessive form of intellectual disability. [provided by RefSeq, May 2017]