DUSP12 (NM_007240) Human Tagged ORF Clone

CAT#: RG202411

DUSP12 (GFP-tagged) - Human dual specificity phosphatase 12 (DUSP12)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DUSP12
• Synonyms: DUSP1; YVH1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202411 representing NM_007240
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGAGGCTCCGGGCCCGAGTGATGGCTGCGAGCTCAGCAACCCCAGCGCCAGCAGAGTCAGCTGTG
  CCGGGCAGATGCTGGAAGTGCAGCCAGGATTGTATTTCGGTGGGGCCGCGGCCGTCGCGGAGCCAGATCA
  CCTGAGGGAAGCGGGCATCACGGCCGTGCTAACAGTGGACTCGGAGGAGCCCAGCTTCAAGGCGGGGCCT
  GGGGTCGAGGATCTATGGCGCCTCTTCGTGCCAGCGCTGGACAAACCCGAGACGGACCTACTCAGCCATC
  TGGACCGGTGCGTGGCCTTCATCGGTCAGGCCCGCGCTGAGGGCCGTGCGGTGTTGGTGCACTGTCATGC
  AGGAGTCAGTCGAAGTGTGGCCATAATAACTGCTTTTCTCATGAAGACTGACCAACTTCCCTTTGAAAAA
  GCCTATGAAAAGCTCCAGATTCTCAAACCAGAGGCTAAGATGAATGAGGGGTTTGAGTGGCAACTGAAAT
  TATACCAGGCAATGGGATATGAAGTGGATACCTCTAGTGCAATTTATAAGCAATATCGTTTACAAAAGGT
  TACAGAGAAGTATCCAGAATTGCAGAATTTACCTCAAGAACTCTTTGCTGTTGACCCAACTACCGTTTCA
  CAAGGATTGAAAGATGAGGTTCTCTACAAGTGTAGAAAGTGCAGGCGATCATTATTTCGAAGTTCTAGTA
  TTCTGGATCACCGTGAAGGAAGTGGACCTATAGCCTTTGCCCACAAGAGAATGACACCATCTTCCATGCT
  TACCACAGGGAGGCAAGCTCAATGTACATCTTATTTCATTGAACCTGTACAGTGGATGGAATCTGCTTTG
  TTGGGAGTGATGGATGGACAGCTTCTTTGCCCAAAATGCAGTGCCAAGTTGGGTTCCTTCAACTGGTATG
  GTGAACAGTGCTCTTGTGGTAGGTGGATAACACCTGCTTTTCAAATACATAAGAATAGAGTGGATGAAAT
  GAAAATATTGCCTGTTTTGGGATCACAAACAGGAAAAATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202411 representing NM_007240
  Red=Cloning site Green=Tags(s)
  
  MLEAPGPSDGCELSNPSASRVSCAGQMLEVQPGLYFGGAAAVAEPDHLREAGITAVLTVDSEEPSFKAGP
  GVEDLWRLFVPALDKPETDLLSHLDRCVAFIGQARAEGRAVLVHCHAGVSRSVAIITAFLMKTDQLPFEK
  AYEKLQILKPEAKMNEGFEWQLKLYQAMGYEVDTSSAIYKQYRLQKVTEKYPELQNLPQELFAVDPTTVS
  QGLKDEVLYKCRKCRRSLFRSSSILDHREGSGPIAFAHKRMTPSSMLTTGRQAQCTSYFIEPVQWMESAL
  LGVMDGQLLCPKCSAKLGSFNWYGEQCSCGRWITPAFQIHKNRVDEMKILPVLGSQTGKI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007240
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007240.1, NP_009171.1
• RefSeq Size: 1271
• RefSeq ORF: 1023
• Locus ID: 11266
• Domains: DSPc
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product is the human ortholog of the Saccharomyces cerevisiae YVH1 protein tyrosine phosphatase. It is localized predominantly in the nucleus, and is novel in that it contains, and is regulated by a zinc finger domain. [provided by RefSeq, Jul 2008]