Carboxypeptidase A (CPA1) (NM_001868) Human Tagged ORF Clone

CAT#: RG202720

CPA1 (GFP-tagged) - Human carboxypeptidase A1 (pancreatic) (CPA1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CPA1
• Synonyms: CPA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202720 representing NM_001868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGGTTGCTGGTGTTGAGTGTCCTGTTGGGGGCTGTCTTTGGCAAGGAGGACTTTGTGGGGCATC
  AGGTGCTCCGAATCTCTGTAGCCGATGAGGCCCAGGTACAGAAGGTGAAGGAGCTGGAGGACCTGGAGCA
  CCTGCAGCTGGACTTCTGGCGGGGCCCTGCCCACCCTGGCTCCCCCATCGACGTCCGAGTGCCCTTCCCC
  AGCATCCAGGCGGTCAAGATCTTTCTGGAGTCCCACGGCATCAGCTATGAGACCATGATCGAGGACGTGC
  AGTCGCTGCTGGACGAGGAGCAGGAGCAGATGTTCGCCTTCCGGTCCCGGGCGCGCTCCACCGACACTTT
  TAACTACGCCACCTACCACACCCTGGAGGAGATCTATGACTTCCTGGACCTGCTGGTGGCGGAGAACCCG
  CACCTTGTCAGCAAGATCCAGATTGGCAACACCTATGAAGGGCGTCCCATTTACGTGCTGAAGTTCAGCA
  CGGGGGGCAGTAAGCGTCCAGCCATCTGGATCGACACGGGCATCCATTCCCGGGAGTGGGTCACCCAGGC
  CAGTGGGGTCTGGTTTGCAAAGAAGATCACTCAAGACTACGGGCAGGATGCAGCTTTCACCGCCATTCTC
  GACACCTTGGACATCTTCCTGGAGATCGTCACCAACCCTGATGGCTTTGCCTTCACGCACAGCACGAATC
  GCATGTGGCGCAAGACTCGGTCCCACACAGCAGGCTCCCTCTGTATTGGCGTGGACCCCAACAGGAACTG
  GGACGCTGGCTTTGGGTTGTCCGGAGCCAGCAGTAACCCCTGCTCGGAGACTTACCGCGGCAAGTTTGCC
  AATTCCGAAGTGGAGGTCAAGTCCATTGTAGACTTTGTGAAGGACCATGGGAACATCAAGGCCTTCATCT
  CCATCCACAGCTACTCCCAGCTCCTCATGTATCCCTATGGCTACAAAACAGAACCAGTCCCTGACCAGGA
  TGAGCTGGATCAGCTTTCCAAGGCTGCTGTGACAGCCCTGGCCTCTCTCTACGGGACCAAGTTCAACTAT
  GGCAGCATCATCAAGGCAATTTATCAAGCCAGTGGAAGCACTATTGACTGGACCTACAGCCAGGGCATCA
  AGTACTCCTTCACCTTCGAGCTCCGGGACACTGGGCGCTATGGCTTCCTGCTGCCAGCCTCCCAGATCAT
  CCCCACAGCCAAGGAGACGTGGCTGGCGCTTCTGACCATCATGGAGCACACCCTGAATCACCCCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202720 representing NM_001868
  Red=Cloning site Green=Tags(s)
  
  MRGLLVLSVLLGAVFGKEDFVGHQVLRISVADEAQVQKVKELEDLEHLQLDFWRGPAHPGSPIDVRVPFP
  SIQAVKIFLESHGISYETMIEDVQSLLDEEQEQMFAFRSRARSTDTFNYATYHTLEEIYDFLDLLVAENP
  HLVSKIQIGNTYEGRPIYVLKFSTGGSKRPAIWIDTGIHSREWVTQASGVWFAKKITQDYGQDAAFTAIL
  DTLDIFLEIVTNPDGFAFTHSTNRMWRKTRSHTAGSLCIGVDPNRNWDAGFGLSGASSNPCSETYRGKFA
  NSEVEVKSIVDFVKDHGNIKAFISIHSYSQLLMYPYGYKTEPVPDQDELDQLSKAAVTALASLYGTKFNY
  GSIIKAIYQASGSTIDWTYSQGIKYSFTFELRDTGRYGFLLPASQIIPTAKETWLALLTIMEHTLNHPY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001868
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001868.1, NP_001859.1
• RefSeq Size: 1380
• RefSeq ORF: 1260
• Locus ID: 1357
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Secreted Protein
• Gene Summary: This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer. [provided by RefSeq, Jan 2015]