SULT1C2 (NM_001056) Human Tagged ORF Clone

CAT#: RG202775

SULT1C2 (GFP-tagged) - Human sulfotransferase family, cytosolic, 1C, member 2 (SULT1C2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SULT1C2
• Synonyms: humSULTC2; ST1C1; ST1C2; SULT1C1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202775 representing NM_001056
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGACCTCAGACCTGGGGAAACAGATAAAACTGAAAGAGGTGGAGGGGACCCTCCTGCAGCCTG
  CAACTGTGGACAACTGGAGCCAGATCCAGAGCTTCGAGGCCAAACCAGATGATCTCCTCATCTGCACCTA
  CCCTAAAGCAGGGACAACGTGGATTCAGGAAATTGTGGATATGATTGAACAGAATGGGGACGTGGAGAAG
  TGCCAGCGAGCCATCATCCAACACCGCCATCCTTTCATTGAGTGGGCTCGGCCACCCCAACCTTCTGGTG
  TGGAAAAAGCCAAAGCAATGCCCTCTCCACGGATACTAAAGACTCACCTTTCCACTCAGCTGCTGCCACC
  GTCTTTCTGGGAAAACAACTGCAAGTTCCTTTATGTAGCTCGAAATGCCAAAGACTGTATGGTTTCCTAC
  TACCATTTCCAAAGGATGAACCACATGCTTCCTGACCCTGGTACCTGGGAAGAGTATTTTGAAACCTTCA
  TCAATGGAAAAGTGGTTTGGGGTTCCTGGTTTGACCACGTGAAAGGATGGTGGGAGATGAAAGACAGACA
  CCAGATTCTCTTCCTCTTCTATGAGGACATAAAGAGGGACCCAAAGCATGAAATTCGGAAGGTGATGCAG
  TTCATGGGAAAGAAGGTGGATGAAACAGTGCTAGATAAAATTGTCCAGGAGACGTCATTTGAGAAAATGA
  AAGAAAATCCCATGACAAATCGTTCTACAGTTTCCAAATCTATCTTGGACCAGTCAATTTCCTCCTTCAT
  GAGAAAAGGAACTGTGGGGGATTGGAAAAACCACTTCACTGTTGCCCAGAATGAGAGGTTTGATGAAATC
  TATAGAAGAAAGATGGAAGGAACCTCCATAAACTTCTGCATGGAACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202775 representing NM_001056
  Red=Cloning site Green=Tags(s)
  
  MALTSDLGKQIKLKEVEGTLLQPATVDNWSQIQSFEAKPDDLLICTYPKAGTTWIQEIVDMIEQNGDVEK
  CQRAIIQHRHPFIEWARPPQPSGVEKAKAMPSPRILKTHLSTQLLPPSFWENNCKFLYVARNAKDCMVSY
  YHFQRMNHMLPDPGTWEEYFETFINGKVVWGSWFDHVKGWWEMKDRHQILFLFYEDIKRDPKHEIRKVMQ
  FMGKKVDETVLDKIVQETSFEKMKENPMTNRSTVSKSILDQSISSFMRKGTVGDWKNHFTVAQNERFDEI
  YRRKMEGTSINFCMEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001056
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001056.3, NP_001047.1
• RefSeq Size: 2799 bp
• RefSeq ORF: 891 bp
• Locus ID: 6819
• Cytogenetics: 2q12.3
• Gene Summary: 'Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that belongs to the SULT1 subfamily, responsible for transferring a sulfo moiety from PAPS to phenol-containing compounds. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'