VAMP3 (NM_004781) Human Tagged ORF Clone

CAT#: RG202783

VAMP3 (GFP-tagged) - Human vesicle-associated membrane protein 3 (cellubrevin) (VAMP3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: VAMP3
• Synonyms: CEB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202783 representing NM_004781
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACAGGTCCAACTGCTGCCACTGGCAGTAATCGAAGACTTCAGCAGACACAAAATCAAGTAGATG
  AGGTGGTGGACATAATGCGAGTTAACGTGGACAAGGTTCTGGAAAGAGACCAGAAGCTCTCTGAGTTAGA
  CGACCGTGCAGACGCACTGCAGGCAGGCGCTTCTCAATTTGAAACGAGCGCAGCCAAGTTGAAGAGGAAA
  TATTGGTGGAAGAATTGCAAGATGTGGGCAATCGGGATTACTGTTCTGGTTATCTTCATCATCATCATCA
  TCGTGTGGGTTGTCTCTTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202783 representing NM_004781
  Red=Cloning site Green=Tags(s)
  
  MSTGPTAATGSNRRLQQTQNQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRK
  YWWKNCKMWAIGITVLVIFIIIIIVWVVSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004781
• ORF Size: 300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004781.3, NP_004772.1
• RefSeq Size: 2225
• RefSeq ORF: 303
• Locus ID: 9341
• Domains: synaptobrevin
• Protein Families: Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]